ID   IMM2_ECOLX              Reviewed;          86 AA.
AC   P04482;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Colicin-E2 immunity protein;
DE   AltName: Full=ImmE2;
DE   AltName: Full=Microcin-E2 immunity protein;
GN   Name=imm; Synonyms=ceiB;
OS   Escherichia coli.
OG   Plasmid ColE2-P9.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6095211; DOI=10.1093/nar/12.22.8733;
RA   Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P.;
RT   "Comparative nucleotide sequences encoding the immunity proteins and the
RT   carboxyl-terminal peptides of colicins E2 and E3.";
RL   Nucleic Acids Res. 12:8733-8745(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987833; DOI=10.1093/nar/13.5.1623;
RA   Masaki H., Toba M., Ohta T.;
RT   "Structure and expression of the ColE2-P9 immunity gene.";
RL   Nucleic Acids Res. 13:1623-1635(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3892228; DOI=10.1007/bf00332940;
RA   Cole S.T., Saint-Joanis B., Pugsley A.P.;
RT   "Molecular characterisation of the colicin E2 operon and identification of
RT   its products.";
RL   Mol. Gen. Genet. 198:465-472(1985).
CC   -!- FUNCTION: This protein is able to protect a cell, which harbors the
CC       plasmid ColE2 encoding colicin E2, against colicin E2.
CC   -!- INTERACTION:
CC       P04482; P09883: col; NbExp=4; IntAct=EBI-15855054, EBI-1029888;
CC   -!- SIMILARITY: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2
CC       family. {ECO:0000305}.
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DR   EMBL; X02227; CAA26146.1; -; Genomic_DNA.
DR   EMBL; X01163; CAA25610.1; -; Genomic_DNA.
DR   EMBL; M29885; AAA23069.1; -; Genomic_DNA.
DR   PIR; A03512; IMECE2.
DR   RefSeq; WP_000420693.1; NZ_VTWF01000215.1.
DR   RefSeq; YP_001966153.1; NC_010883.1.
DR   RefSeq; YP_002221665.1; NC_011228.1.
DR   PDB; 2NO8; NMR; -; A=1-86.
DR   PDB; 2WPT; X-ray; 1.78 A; A=1-86.
DR   PDB; 3U43; X-ray; 1.72 A; A=1-86.
DR   PDBsum; 2NO8; -.
DR   PDBsum; 2WPT; -.
DR   PDBsum; 3U43; -.
DR   AlphaFoldDB; P04482; -.
DR   BMRB; P04482; -.
DR   SMR; P04482; -.
DR   DIP; DIP-59336N; -.
DR   IntAct; P04482; 1.
DR   EvolutionaryTrace; P04482; -.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   CDD; cd16363; Col_Im_like; 1.
DR   Gene3D; 1.10.1200.20; -; 1.
DR   InterPro; IPR035900; Colicin_E_sf.
DR   InterPro; IPR000290; Colicin_pyocin.
DR   Pfam; PF01320; Colicin_Pyocin; 1.
DR   PRINTS; PR01299; PYOCIN.
DR   SUPFAM; SSF47345; SSF47345; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Bacteriocin immunity; Plasmid.
FT   CHAIN           1..86
FT                   /note="Colicin-E2 immunity protein"
FT                   /id="PRO_0000218703"
FT   HELIX           7..9
FT                   /evidence="ECO:0007829|PDB:3U43"
FT   HELIX           12..24
FT                   /evidence="ECO:0007829|PDB:3U43"
FT   STRAND          27..29
FT                   /evidence="ECO:0007829|PDB:2WPT"
FT   HELIX           30..44
FT                   /evidence="ECO:0007829|PDB:3U43"
FT   TURN            47..50
FT                   /evidence="ECO:0007829|PDB:3U43"
FT   HELIX           51..54
FT                   /evidence="ECO:0007829|PDB:3U43"
FT   STRAND          58..60
FT                   /evidence="ECO:0007829|PDB:2NO8"
FT   HELIX           64..77
FT                   /evidence="ECO:0007829|PDB:3U43"
SQ   SEQUENCE   86 AA;  9995 MW;  1C5C89ED7E87BC39 CRC64;
     MELKHSISDY TEAEFLEFVK KICRAEGATE EDDNKLVREF ERLTEHPDGS DLIYYPRDDR
     EDSPEGIVKE IKEWRAANGK SGFKQG