IMM3_ECOLX
ID IMM3_ECOLX Reviewed; 85 AA.
AC P02984;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Colicin-E3 immunity protein;
DE AltName: Full=Colicin-E3 chain B;
DE AltName: Full=ImmE3;
DE AltName: Full=Microcin-E3 immunity protein;
GN Name=imm; Synonyms=ceiC, immB;
OS Escherichia coli.
OG Plasmid ColE3-CA38.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6295812; DOI=10.1016/0014-5793(82)81087-9;
RA Masaki H., Ohta T.;
RT "A plasmid region encoding the active fragment and the inhibitor protein of
RT colicin E3-CA38.";
RL FEBS Lett. 149:129-132(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3889348; DOI=10.1016/0022-2836(85)90340-7;
RA Masaki H., Ohta T.;
RT "Colicin E3 and its immunity genes.";
RL J. Mol. Biol. 182:217-227(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6344012; DOI=10.1093/nar/11.11.3547;
RA Mock M., Miyada C.G., Gunsalus R.P.;
RT "Nucleotide sequence for the catalytic domain of colicin E3 and its
RT immunity protein. Evidence for a third gene overlapping colicin.";
RL Nucleic Acids Res. 11:3547-3557(1983).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6095211; DOI=10.1093/nar/12.22.8733;
RA Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P.;
RT "Comparative nucleotide sequences encoding the immunity proteins and the
RT carboxyl-terminal peptides of colicins E2 and E3.";
RL Nucleic Acids Res. 12:8733-8745(1984).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6383494; DOI=10.1007/bf01121913;
RA Lau P.C., Rowsome R.W., Watson R.J., Visentin L.P.;
RT "The immunity genes of colicins E2 and E8 are closely related.";
RL Biosci. Rep. 4:565-572(1984).
RN [6]
RP STRUCTURE BY NMR.
RX PubMed=1610804; DOI=10.1021/bi00139a022;
RA Yajima S., Muto Y., Yokoyama S., Masaki H., Uozumi T.;
RT "The secondary structure of the colicin E3 immunity protein as studied by
RT 1H-1H and 1H-15N two-dimensional NMR spectroscopy.";
RL Biochemistry 31:5578-5586(1992).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
RX PubMed=10574790; DOI=10.1016/s0969-2126(00)80026-x;
RA Li C., Zhao D., Djebli A., Shoham M.;
RT "Crystal structure of colicin E3 immunity protein: an inhibitor of a
RT ribosome-inactivating RNase.";
RL Structure 7:1365-1372(1999).
CC -!- FUNCTION: This protein inhibits the 16S RNA hydrolyzing activity of
CC colicin E3 by binding with high affinity to the C-terminal catalytic
CC domain of E3. This protein is able to protect a cell, which harbors the
CC plasmid ColE3 against colicin E3.
CC -!- INTERACTION:
CC P02984; P00646: ceaC; NbExp=2; IntAct=EBI-1029912, EBI-1029919;
CC -!- SIMILARITY: Belongs to the cloacin immunity protein family.
CC {ECO:0000305}.
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DR EMBL; J01574; AAA88417.1; -; Genomic_DNA.
DR EMBL; X02397; CAA26242.1; -; Genomic_DNA.
DR EMBL; X01162; CAA25608.1; -; Genomic_DNA.
DR EMBL; M26136; AAA98285.1; -; Genomic_DNA.
DR PIR; B91307; IMECE3.
DR RefSeq; WP_000523346.1; NZ_RRSO01000112.1.
DR PDB; 1E44; X-ray; 2.40 A; A=1-85.
DR PDB; 1JCH; X-ray; 3.02 A; B/D=2-85.
DR PDB; 2B5U; X-ray; 2.30 A; B/D=2-85.
DR PDB; 3EIP; X-ray; 1.80 A; A/B=2-85.
DR PDB; 4UDM; X-ray; 2.96 A; A=1-85.
DR PDBsum; 1E44; -.
DR PDBsum; 1JCH; -.
DR PDBsum; 2B5U; -.
DR PDBsum; 3EIP; -.
DR PDBsum; 4UDM; -.
DR AlphaFoldDB; P02984; -.
DR SMR; P02984; -.
DR DIP; DIP-16993N; -.
DR IntAct; P02984; 1.
DR TCDB; 8.B.24.4.1; the colicin immunity protein (colip) functional family.
DR EvolutionaryTrace; P02984; -.
DR GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR Gene3D; 3.10.50.20; -; 1.
DR InterPro; IPR003063; Cloacn_immnty_fam.
DR InterPro; IPR036528; Cloacn_immnty_sf.
DR Pfam; PF03513; Cloacin_immun; 1.
DR PRINTS; PR01296; CLOACNIMMNTY.
DR SUPFAM; SSF54552; SSF54552; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriocin immunity; Plasmid.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..85
FT /note="Colicin-E3 immunity protein"
FT /id="PRO_0000218704"
FT STRAND 3..11
FT /evidence="ECO:0007829|PDB:3EIP"
FT TURN 12..14
FT /evidence="ECO:0007829|PDB:3EIP"
FT STRAND 17..22
FT /evidence="ECO:0007829|PDB:3EIP"
FT HELIX 31..34
FT /evidence="ECO:0007829|PDB:3EIP"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:3EIP"
FT HELIX 40..43
FT /evidence="ECO:0007829|PDB:3EIP"
FT STRAND 48..50
FT /evidence="ECO:0007829|PDB:3EIP"
FT HELIX 53..55
FT /evidence="ECO:0007829|PDB:3EIP"
FT HELIX 56..59
FT /evidence="ECO:0007829|PDB:3EIP"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:3EIP"
FT TURN 69..71
FT /evidence="ECO:0007829|PDB:3EIP"
FT STRAND 72..80
FT /evidence="ECO:0007829|PDB:3EIP"
SQ SEQUENCE 85 AA; 9904 MW; DF5794D36B67ED3D CRC64;
MGLKLDLTWF DKSTEDFKGE EYSKDFGDDG SVMESLGVPF KDNVNNGCFD VIAEWVPLLQ
PYFNHQIDIS DNEYFVSFDY RDGDW
