ID   IMM6_ECOLX              Reviewed;          85 AA.
AC   P13477;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Colicin-E6 immunity protein;
DE   AltName: Full=ImmE6;
DE   AltName: Full=Microcin-E6 immunity protein;
GN   Name=imm; Synonyms=ceiE6;
OS   Escherichia coli.
OG   Plasmid ColE6-CT14.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2549375; DOI=10.1007/bf02464892;
RA   Lau P.C.K., Condie J.A.;
RT   "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a
RT   degenerate transposon-like structure in the ColE9-J plasmid.";
RL   Mol. Gen. Genet. 217:269-277(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2687234; DOI=10.1128/jb.171.12.6430-6436.1989;
RA   Akutsu A., Masaki H., Ohta T.;
RT   "Molecular structure and immunity specificity of colicin E6, an
RT   evolutionary intermediate between E-group colicins and cloacin DF13.";
RL   J. Bacteriol. 171:6430-6436(1989).
CC   -!- FUNCTION: This protein inhibits the 16S RNA hydrolyzing activity of
CC       colicin E6 by binding with high affinity to the C-terminal catalytic
CC       domain of E6. This protein is able to protect a cell, which harbors the
CC       plasmid ColE6 against colicin E6.
CC   -!- SIMILARITY: Belongs to the cloacin immunity protein family.
CC       {ECO:0000305}.
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DR   EMBL; X15856; CAA33856.1; -; Genomic_DNA.
DR   EMBL; M31808; AAA23081.1; -; Genomic_DNA.
DR   PIR; JQ0326; JQ0326.
DR   AlphaFoldDB; P13477; -.
DR   SMR; P13477; -.
DR   PRIDE; P13477; -.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.50.20; -; 1.
DR   InterPro; IPR003063; Cloacn_immnty_fam.
DR   InterPro; IPR036528; Cloacn_immnty_sf.
DR   Pfam; PF03513; Cloacin_immun; 1.
DR   PRINTS; PR01296; CLOACNIMMNTY.
DR   SUPFAM; SSF54552; SSF54552; 1.
PE   3: Inferred from homology;
KW   Bacteriocin immunity; Plasmid.
FT   CHAIN           1..85
FT                   /note="Colicin-E6 immunity protein"
FT                   /id="PRO_0000218707"
FT   CONFLICT        13
FT                   /note="R -> T (in Ref. 2; AAA23081)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        40
FT                   /note="F -> L (in Ref. 2; AAA23081)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   85 AA;  10130 MW;  C6C78D1F2577D649 CRC64;
     MGLKLHINWF DKRTEEFKGG EYSKDFGDDG SVIERLGMPF KDNINNGWFD VIAEWVPLLQ
     PYFNHQIDIS DNEYFVSFDY RDGDW