ID   IMM8_ECOLX              Reviewed;          85 AA.
AC   P09881;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Colicin-E8 immunity protein;
DE   AltName: Full=ImmE8;
DE   AltName: Full=Microcin-E8 immunity protein;
GN   Name=imm; Synonyms=ceiE8;
OS   Escherichia coli.
OG   Plasmid ColE8.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3323826; DOI=10.1007/bf00331154;
RA   Uchimura T., Lau P.C.K.;
RT   "Nucleotide sequences from the colicin E8 operon: homology with plasmid
RT   ColE2-P9.";
RL   Mol. Gen. Genet. 209:489-493(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3290201; DOI=10.1128/jb.170.7.3237-3242.1988;
RA   Toba M., Masaki H., Ohta T.;
RT   "Colicin E8, a DNase which indicates an evolutionary relationship between
RT   colicins E2 and E3.";
RL   J. Bacteriol. 170:3237-3242(1988).
RN   [3]
RP   STRUCTURE BY NMR.
RA   Videler H., Boetzel R., Osborne M.J., Williams G., Lian L.Y., James R.,
RA   Kleanthous C., Moore G.R.;
RL   Submitted (JAN-1997) to the PDB data bank.
CC   -!- FUNCTION: This protein is able to protect a cell, which harbors the
CC       plasmid ColE8 encoding colicin E8, against colicin E8, it binds
CC       specifically to the DNase-type colicin and inhibits its bactericidal
CC       activity.
CC   -!- SIMILARITY: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2
CC       family. {ECO:0000305}.
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DR   EMBL; M21404; AAA23074.1; -; Genomic_DNA.
DR   EMBL; X06119; CAA29492.1; -; Genomic_DNA.
DR   PIR; B28184; IMECE8.
DR   RefSeq; WP_000421100.1; NZ_VTNB01000010.1.
DR   RefSeq; YP_002993420.1; NC_012882.1.
DR   PDB; 1GXG; NMR; -; A=1-85.
DR   PDB; 1GXH; NMR; -; A=1-85.
DR   PDBsum; 1GXG; -.
DR   PDBsum; 1GXH; -.
DR   AlphaFoldDB; P09881; -.
DR   SMR; P09881; -.
DR   EvolutionaryTrace; P09881; -.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   CDD; cd16363; Col_Im_like; 1.
DR   Gene3D; 1.10.1200.20; -; 1.
DR   InterPro; IPR035900; Colicin_E_sf.
DR   InterPro; IPR000290; Colicin_pyocin.
DR   Pfam; PF01320; Colicin_Pyocin; 1.
DR   PRINTS; PR01299; PYOCIN.
DR   SUPFAM; SSF47345; SSF47345; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Bacteriocin immunity; Plasmid.
FT   CHAIN           1..85
FT                   /note="Colicin-E8 immunity protein"
FT                   /id="PRO_0000218709"
FT   TURN            7..9
FT                   /evidence="ECO:0007829|PDB:1GXG"
FT   HELIX           12..24
FT                   /evidence="ECO:0007829|PDB:1GXG"
FT   STRAND          26..29
FT                   /evidence="ECO:0007829|PDB:1GXG"
FT   HELIX           30..41
FT                   /evidence="ECO:0007829|PDB:1GXG"
FT   TURN            46..49
FT                   /evidence="ECO:0007829|PDB:1GXG"
FT   HELIX           50..53
FT                   /evidence="ECO:0007829|PDB:1GXG"
FT   HELIX           63..76
FT                   /evidence="ECO:0007829|PDB:1GXG"
SQ   SEQUENCE   85 AA;  9623 MW;  E31C481EFF6B4A6E CRC64;
     MELKNSISDY TETEFKKIIE DIINCEGDEK KQDDNLEHFI SVTEHPSGSD LIYYPEGNND
     GSPEAVIKEI KEWRAANGKS GFKQG