IMM9_ECOLX
ID IMM9_ECOLX Reviewed; 86 AA.
AC P13479;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Colicin-E9 immunity protein;
DE AltName: Full=ImmE9;
DE AltName: Full=Microcin-E9 immunity protein;
GN Name=imm; Synonyms=ceiE9;
OS Escherichia coli.
OG Plasmid ColE9-J.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
RX PubMed=2549375; DOI=10.1007/bf02464892;
RA Lau P.C.K., Condie J.A.;
RT "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a
RT degenerate transposon-like structure in the ColE9-J plasmid.";
RL Mol. Gen. Genet. 217:269-277(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Lau P.C.K.;
RL Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3312476; DOI=10.1099/00221287-133-6-1553;
RA James R., Jarvis M., Barker D.F.;
RT "Nucleotide sequence of the immunity and lysis region of the ColE9-J
RT plasmid.";
RL J. Gen. Microbiol. 133:1553-1562(1987).
RN [4]
RP STRUCTURE BY NMR.
RX PubMed=8755730; DOI=10.1021/bi960401k;
RA Osborne M.J., Breeze A.L., Lian L.Y., Reilly A., James R., Kleanthous C.,
RA Moore G.R.;
RT "Three-dimensional solution structure and 13C nuclear magnetic resonance
RT assignments of the colicin E9 immunity protein Im9.";
RL Biochemistry 35:9505-9512(1996).
CC -!- FUNCTION: This protein is able to protect a cell, which harbors the
CC plasmid ColE9 encoding colicin E9, against colicin E9, it binds
CC specifically to the DNase-type colicin and inhibits its bactericidal
CC activity.
CC -!- INTERACTION:
CC P13479; P09883: col; NbExp=4; IntAct=EBI-1029882, EBI-1029888;
CC P13479; Q47112: colE7; NbExp=3; IntAct=EBI-1029882, EBI-1035016;
CC -!- SIMILARITY: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2
CC family. {ECO:0000305}.
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DR EMBL; X15858; CAA33863.1; -; Genomic_DNA.
DR EMBL; M16803; AAA23076.1; -; Genomic_DNA.
DR PIR; A32535; A32535.
DR RefSeq; WP_012644887.1; NZ_RRDY01000191.1.
DR RefSeq; YP_002533538.1; NC_011977.1.
DR PDB; 1BXI; X-ray; 2.05 A; A=1-86.
DR PDB; 1E0H; NMR; -; A=1-86.
DR PDB; 1EMV; X-ray; 1.70 A; A=1-86.
DR PDB; 1FR2; X-ray; 1.60 A; A=1-86.
DR PDB; 1IMP; NMR; -; A=1-86.
DR PDB; 1IMQ; NMR; -; A=1-86.
DR PDB; 2GYK; X-ray; 1.60 A; A/E=1-86.
DR PDB; 2GZE; X-ray; 1.80 A; A=1-86.
DR PDB; 2GZF; X-ray; 1.75 A; A=1-86.
DR PDB; 2GZG; X-ray; 1.70 A; A=1-86.
DR PDB; 2GZI; X-ray; 1.70 A; A=1-86.
DR PDB; 2GZJ; X-ray; 1.60 A; A/E=1-86.
DR PDB; 2K5X; NMR; -; A=1-86.
DR PDB; 2VLN; X-ray; 1.60 A; A=1-86.
DR PDB; 2VLO; X-ray; 1.80 A; A=1-86.
DR PDB; 2VLP; X-ray; 2.00 A; A=1-86.
DR PDB; 2VLQ; X-ray; 1.60 A; A=1-86.
DR PDB; 3GJN; X-ray; 2.48 A; A/D=1-86.
DR PDB; 3GKL; X-ray; 2.20 A; C/D=1-86.
DR PDB; 5EW5; X-ray; 3.20 A; E/F/G/H=1-86.
DR PDBsum; 1BXI; -.
DR PDBsum; 1E0H; -.
DR PDBsum; 1EMV; -.
DR PDBsum; 1FR2; -.
DR PDBsum; 1IMP; -.
DR PDBsum; 1IMQ; -.
DR PDBsum; 2GYK; -.
DR PDBsum; 2GZE; -.
DR PDBsum; 2GZF; -.
DR PDBsum; 2GZG; -.
DR PDBsum; 2GZI; -.
DR PDBsum; 2GZJ; -.
DR PDBsum; 2K5X; -.
DR PDBsum; 2VLN; -.
DR PDBsum; 2VLO; -.
DR PDBsum; 2VLP; -.
DR PDBsum; 2VLQ; -.
DR PDBsum; 3GJN; -.
DR PDBsum; 3GKL; -.
DR PDBsum; 5EW5; -.
DR AlphaFoldDB; P13479; -.
DR BMRB; P13479; -.
DR SMR; P13479; -.
DR DIP; DIP-16991N; -.
DR IntAct; P13479; 2.
DR EvolutionaryTrace; P13479; -.
DR GO; GO:0032991; C:protein-containing complex; IDA:CAFA.
DR GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR CDD; cd16363; Col_Im_like; 1.
DR Gene3D; 1.10.1200.20; -; 1.
DR InterPro; IPR035900; Colicin_E_sf.
DR InterPro; IPR000290; Colicin_pyocin.
DR Pfam; PF01320; Colicin_Pyocin; 1.
DR PRINTS; PR01299; PYOCIN.
DR SUPFAM; SSF47345; SSF47345; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriocin immunity; Plasmid.
FT CHAIN 1..86
FT /note="Colicin-E9 immunity protein"
FT /id="PRO_0000218711"
FT CONFLICT 26
FT /note="D -> N (in Ref. 3; AAA23076)"
FT /evidence="ECO:0000305"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:1FR2"
FT HELIX 12..23
FT /evidence="ECO:0007829|PDB:1FR2"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:1FR2"
FT HELIX 30..44
FT /evidence="ECO:0007829|PDB:1FR2"
FT TURN 47..50
FT /evidence="ECO:0007829|PDB:1FR2"
FT HELIX 51..54
FT /evidence="ECO:0007829|PDB:1FR2"
FT HELIX 64..77
FT /evidence="ECO:0007829|PDB:1FR2"
SQ SEQUENCE 86 AA; 9583 MW; 136CBD25D071FDB4 CRC64;
MELKHSISDY TEAEFLQLVT TICNADTSSE EELVKLVTHF EEMTEHPSGS DLIYYPKEGD
DDSPSGIVNT VKQWRAANGK SGFKQG
