ID   IMMA_CITFR              Reviewed;         178 AA.
AC   P05701;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Colicin-A immunity protein;
DE   AltName: Full=Microcin-A immunity protein;
GN   Name=cai;
OS   Citrobacter freundii.
OG   Plasmid ColA-CA31.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6383827; DOI=10.1111/j.1432-1033.1984.tb08432.x;
RA   Lloubes R.P., Chartier M.J., Journet A.M., Varenne S.G., Lazdunski C.J.;
RT   "Nucleotide sequence of the gene for the immunity protein to colicin A.
RT   Analysis of codon usage of immunity proteins as compared to colicins.";
RL   Eur. J. Biochem. 144:73-78(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2832701; DOI=10.1007/bf00330599;
RA   Morlon J., Chartier M., Bidaud M., Lazdunski C.;
RT   "The complete nucleotide sequence of the colicinogenic plasmid ColA. High
RT   extent of homology with ColE1.";
RL   Mol. Gen. Genet. 211:231-243(1988).
RN   [3]
RP   TOPOLOGY, AND FUNCTION.
RX   PubMed=2668695; DOI=10.1111/j.1365-2958.1989.tb00216.x;
RA   Geli V., Baty D., Pattus F., Lazdunski C.;
RT   "Topology and function of the integral membrane protein conferring immunity
RT   to colicin A.";
RL   Mol. Microbiol. 3:679-687(1989).
CC   -!- FUNCTION: This protein is able to protect a cell, which harbors the
CC       plasmid ColA encoding colicin A, against colicin A.
CC       {ECO:0000269|PubMed:2668695}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X00964; CAA25476.1; -; Genomic_DNA.
DR   EMBL; M37402; AAA72880.1; -; Genomic_DNA.
DR   PIR; S07230; IMEBAC.
DR   RefSeq; WP_008323617.1; NC_016151.1.
DR   RefSeq; YP_004933728.1; NC_016151.1.
DR   AlphaFoldDB; P05701; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   InterPro; IPR005557; Colicin_im.
DR   Pfam; PF03857; Colicin_im; 1.
DR   PIRSF; PIRSF003003; Colicin_im; 1.
PE   1: Evidence at protein level;
KW   Bacteriocin immunity; Cell inner membrane; Cell membrane; Membrane;
KW   Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..178
FT                   /note="Colicin-A immunity protein"
FT                   /id="PRO_0000218690"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:2668695"
FT   TRANSMEM        14..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        38..68
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:2668695"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        90..105
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:2668695"
FT   TRANSMEM        106..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        124..142
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:2668695"
FT   TRANSMEM        143..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        166..178
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:2668695"
SQ   SEQUENCE   178 AA;  20464 MW;  1A0D0E6840A1C00D CRC64;
     MMNEHSIDTD NRKANNALYL FIIIGLIPLL CIFVVYYKTP DALLLRKIAT STENLPSITS
     SYNPLMTKVM DIYCKTAPFL ALILYILTFK IRKLINNTDR NTVLRSCLLS PLVYAAIVYL
     FCFRNFELTT AGRPVRLMAT NDATLLLFYI GLYSIIFFTT YITLFTPVTA FKLLKKRQ