ID   IMMC_ECOLX              Reviewed;          85 AA.
AC   P02986;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Cloacin immunity protein;
GN   Name=cim;
OS   Escherichia coli.
OG   Plasmid Clo DF13.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3749334; DOI=10.1016/0147-619x(86)90072-7;
RA   Nijkamp H.J.J., de Lang R., Stuitje A.R., van den Elsen P.J.M.,
RA   Veltkamp E., van Putten A.J.;
RT   "The complete nucleotide sequence of the bacteriocinogenic plasmid
RT   CloDF13.";
RL   Plasmid 16:135-160(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6163089; DOI=10.1038/290264a0;
RA   Stuitje A.R., Spelt C.E., Veltkamp E., Nijkamp H.J.J.;
RT   "Identification of mutations affecting replication control of plasmid Clo
RT   DF13.";
RL   Nature 290:264-267(1981).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND METHYLATION AT LYS-12.
RX   PubMed=6253914; DOI=10.1093/nar/8.19.4349;
RA   van den Elzen P.J.M., Gaastra W., Spelt C.E., de Graaf F.K., Veltkamp E.,
RA   Nijkamp H.J.J.;
RT   "Molecular structure of the immunity gene and immunity protein of the
RT   bacteriocinogenic plasmid Clo DF13.";
RL   Nucleic Acids Res. 8:4349-4363(1980).
CC   -!- FUNCTION: This protein complexes with cloacin protein in equimolar
CC       amounts and inhibits it by binding with high affinity to the C-terminal
CC       catalytic domain of cloacin.
CC   -!- MISCELLANEOUS: Plasmid Clo DF13 originates from Enterobacter cloacae
CC       but is stably maintained in and studied mostly from E.coli.
CC   -!- SIMILARITY: Belongs to the cloacin immunity protein family.
CC       {ECO:0000305}.
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DR   EMBL; X04466; CAA28146.1; -; Genomic_DNA.
DR   PIR; A93250; IMECP3.
DR   RefSeq; NP_052371.1; NC_002119.1.
DR   RefSeq; WP_010891189.1; NZ_UNQR01000060.1.
DR   AlphaFoldDB; P02986; -.
DR   SMR; P02986; -.
DR   iPTMnet; P02986; -.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.50.20; -; 1.
DR   InterPro; IPR003063; Cloacn_immnty_fam.
DR   InterPro; IPR036528; Cloacn_immnty_sf.
DR   Pfam; PF03513; Cloacin_immun; 1.
DR   PRINTS; PR01296; CLOACNIMMNTY.
DR   SUPFAM; SSF54552; SSF54552; 1.
PE   1: Evidence at protein level;
KW   Bacteriocin immunity; Methylation; Plasmid.
FT   CHAIN           1..85
FT                   /note="Cloacin immunity protein"
FT                   /id="PRO_0000218695"
FT   MOD_RES         12
FT                   /note="N6-methyllysine"
FT                   /evidence="ECO:0000305|PubMed:6253914"
SQ   SEQUENCE   85 AA;  10004 MW;  2F8BCEB9CA8E8E5E CRC64;
     MGLKLHIHWF DKKTEEFKGG EYSKDFGDDG SVIESLGMPL KDNINNGWFD VEKPWVSILQ
     PHFKNVIDIS KFDYFVSFVY RDGNW