ID   IMMI_ECOLX              Reviewed;         111 AA.
AC   P08701;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Colicin-Ia immunity protein;
OS   Escherichia coli.
OG   Plasmid ColIa-CA53, Plasmid ColIa-EC3, Plasmid ColIa-EC28, and
OG   Plasmid ColIa-EC34.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=ColIa-CA53;
RX   PubMed=3531169; DOI=10.1128/jb.168.1.228-236.1986;
RA   Mankovich J.A., Hsu C.-H., Konisky J.;
RT   "DNA and amino acid sequence analysis of structural and immunity genes of
RT   colicins Ia and Ib.";
RL   J. Bacteriol. 168:228-236(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=ColIa-EC28, ColIa-EC3, and ColIa-EC34;
RX   PubMed=7972047; DOI=10.1073/pnas.91.23.11276;
RA   Riley M.A., Tan Y., Wang J.;
RT   "Nucleotide polymorphism in colicin E1 and Ia plasmids from natural
RT   isolates of Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994).
CC   -!- FUNCTION: This protein is able to protect a cell, which harbors the
CC       plasmid ColIa-CA53 encoding colicin Ia, against colicin Ia.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; M13819; AAA23183.1; -; Genomic_DNA.
DR   EMBL; U15622; AAA59397.1; -; Genomic_DNA.
DR   EMBL; U15623; AAA59399.1; -; Genomic_DNA.
DR   EMBL; U15624; AAA59401.1; -; Genomic_DNA.
DR   PIR; E25035; E25035.
DR   RefSeq; WP_001080734.1; NZ_WTNN01000033.1.
DR   RefSeq; YP_006903406.1; NC_019044.1.
DR   AlphaFoldDB; P08701; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   InterPro; IPR003061; Microcin.
DR   Pfam; PF03526; Microcin; 1.
PE   4: Predicted;
KW   Bacteriocin immunity; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..111
FT                   /note="Colicin-Ia immunity protein"
FT                   /id="PRO_0000218693"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   111 AA;  13283 MW;  059F42A8486FC54E CRC64;
     MNRKYYFNNM WWGWVTGGYM LYMSWDYEFK YRLLFWCISL CGMVLYPVAK WYIEDTALKF
     TRPDFWNSGF FADTPGKMGL LAVYTGTVFI LSLPLSMIYI LSVIIKRLSV R