ID   IMMM_ECOLX              Reviewed;         141 AA.
AC   P18002;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 2.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Colicin-M immunity protein;
DE   AltName: Full=Microcin-M immunity protein;
GN   Name=cmi;
OS   Escherichia coli.
OG   Plasmid ColBM-Cl139.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
RX   PubMed=2853881; DOI=10.1016/0147-619x(88)90009-1;
RA   Thumm G., Oelschlaeger T., Braun V.;
RT   "Plasmid pColBM-Cl139 does not encode a colicin lysis protein but contains
RT   sequences highly homologous to the D protein (resolvase) and the oriV
RT   region of the miniF plasmid.";
RL   Plasmid 20:75-82(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-141.
RX   PubMed=2820942; DOI=10.1128/jb.169.10.4765-4769.1987;
RA   Oelschlaeger T., Braun V.;
RT   "Sequence, expression, and localization of the immunity protein for colicin
RT   M.";
RL   J. Bacteriol. 169:4765-4769(1987).
CC   -!- FUNCTION: This protein is able to protect a cell, which harbors the
CC       plasmid ColBM-Cl139 encoding colicin M, against colicin M.
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DR   EMBL; M35683; AAA23011.1; -; Genomic_DNA.
DR   EMBL; M17810; AAA23594.1; -; Genomic_DNA.
DR   PIR; A30479; A30479.
DR   PDB; 2XGL; X-ray; 2.70 A; A/B=48-141.
DR   PDB; 4AEQ; X-ray; 1.89 A; A=44-141.
DR   PDBsum; 2XGL; -.
DR   PDBsum; 4AEQ; -.
DR   AlphaFoldDB; P18002; -.
DR   SMR; P18002; -.
DR   TCDB; 8.B.24.3.1; the colicin immunity protein (colip) functional family.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.450.300; -; 1.
DR   InterPro; IPR038703; YebF/Cmi_sf.
DR   InterPro; IPR025603; YebF/ColM_immunity.
DR   Pfam; PF13995; YebF; 1.
DR   PROSITE; PS51979; YEBF_CMI; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Bacteriocin immunity; Disulfide bond; Plasmid.
FT   CHAIN           1..141
FT                   /note="Colicin-M immunity protein"
FT                   /id="PRO_0000218697"
FT   DOMAIN          51..141
FT                   /note="YebF/Cmi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01323"
FT   DISULFID        55..131
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01323"
FT   HELIX           55..57
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   HELIX           60..85
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   STRAND          91..94
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   STRAND          101..103
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   HELIX           104..106
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   STRAND          109..117
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   STRAND          122..130
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   TURN            131..134
FT                   /evidence="ECO:0007829|PDB:4AEQ"
FT   STRAND          135..139
FT                   /evidence="ECO:0007829|PDB:4AEQ"
SQ   SEQUENCE   141 AA;  16692 MW;  F64477DBB910AF02 CRC64;
     MLTLYGYIRN VFLYRMNDRS CGDFMKVISM KFIFILTIIA LAAVFFWSED KGPACYQVSD
     EQARTFVKND YLQRMKRWDN DVQLLGTEIP KITWEKIERS LTDVEDEKTL LVPFKAEGPD
     GKRMYYGMYH CEEGYVEYAN D