IMPA4_ARATH
ID IMPA4_ARATH Reviewed; 538 AA.
AC O80480; F4HZG6; O49602; Q94KD4;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 163.
DE RecName: Full=Importin subunit alpha-4 {ECO:0000305};
DE Short=IMPa-4 {ECO:0000303|PubMed:18836040};
GN Name=IMPA4 {ECO:0000303|PubMed:18836040};
GN OrderedLocusNames=At1g09270 {ECO:0000312|Araport:AT1G09270};
GN ORFNames=T12M4.2 {ECO:0000312|EMBL:AAC24079.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-537 (ISOFORM 1).
RA Schledz M., Leclerc D., Neuhaus G., Merkle T.;
RT "Characterization of four cDNAs encoding different importin alpha
RT homologues from Arabidopsis thaliana, designated AtIMPa1-4.";
RL (er) Plant Gene Register PGR98-022(1998).
RN [6]
RP FUNCTION, INTERACTION WITH AGROBACTERIUM VIRD2 AND VIRE2, AND GENE FAMILY.
RX PubMed=18836040; DOI=10.1105/tpc.108.060467;
RA Bhattacharjee S., Lee L.Y., Oltmanns H., Cao H., Gupta V., Cuperus J.,
RA Gelvin S.B.;
RT "IMPa-4, an Arabidopsis importin alpha isoform, is preferentially involved
RT in agrobacterium-mediated plant transformation.";
RL Plant Cell 20:2661-2680(2008).
CC -!- FUNCTION: Binds to conventional NLS motifs and mediates nuclear protein
CC import across the nuclear envelope. Acts as cellular receptor for the
CC nuclear import of the virD2 protein of Agrobacterium and is essential
CC for Agrobacterium-mediated root transformation.
CC {ECO:0000269|PubMed:18836040}.
CC -!- SUBUNIT: Forms a complex with importin subunit beta-1 (By similarity).
CC Interacts with A.tumefaciens VirD2 and VirE2 (PubMed:18836040).
CC {ECO:0000250|UniProtKB:Q96321, ECO:0000269|PubMed:18836040}.
CC -!- INTERACTION:
CC O80480; Q9SZU7: KAI2; NbExp=3; IntAct=EBI-2131464, EBI-25519488;
CC O80480; Q058P7; NbExp=2; IntAct=EBI-2131464, EBI-4431752;
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000250|UniProtKB:Q96321}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O80480-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O80480-2; Sequence=VSP_057335;
CC -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR EMBL; AC003114; AAC24079.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28421.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28422.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28423.1; -; Genomic_DNA.
DR EMBL; AF367284; AAK56273.1; -; mRNA.
DR EMBL; AY090944; AAM13992.1; -; mRNA.
DR EMBL; AY117313; AAM51388.1; -; mRNA.
DR EMBL; AK316689; BAH19416.1; -; mRNA.
DR EMBL; Y14616; CAA74966.1; -; mRNA.
DR PIR; F86225; F86225.
DR PIR; T52101; T52101.
DR RefSeq; NP_001031011.1; NM_001035934.2. [O80480-2]
DR RefSeq; NP_172398.1; NM_100797.6. [O80480-1]
DR RefSeq; NP_849623.1; NM_179292.3. [O80480-1]
DR AlphaFoldDB; O80480; -.
DR SMR; O80480; -.
DR IntAct; O80480; 9.
DR STRING; 3702.AT1G09270.1; -.
DR PaxDb; O80480; -.
DR PRIDE; O80480; -.
DR ProteomicsDB; 247013; -. [O80480-1]
DR EnsemblPlants; AT1G09270.1; AT1G09270.1; AT1G09270. [O80480-1]
DR EnsemblPlants; AT1G09270.2; AT1G09270.2; AT1G09270. [O80480-1]
DR EnsemblPlants; AT1G09270.3; AT1G09270.3; AT1G09270. [O80480-2]
DR GeneID; 837448; -.
DR Gramene; AT1G09270.1; AT1G09270.1; AT1G09270. [O80480-1]
DR Gramene; AT1G09270.2; AT1G09270.2; AT1G09270. [O80480-1]
DR Gramene; AT1G09270.3; AT1G09270.3; AT1G09270. [O80480-2]
DR KEGG; ath:AT1G09270; -.
DR Araport; AT1G09270; -.
DR TAIR; locus:2195351; AT1G09270.
DR eggNOG; KOG0166; Eukaryota.
DR HOGENOM; CLU_018084_5_0_1; -.
DR InParanoid; O80480; -.
DR OMA; MTEMIFS; -.
DR PhylomeDB; O80480; -.
DR PRO; PR:O80480; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80480; baseline and differential.
DR Genevisible; O80480; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0043657; C:host cell; IEA:GOC.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0080034; P:host response to induction by symbiont of tumor, nodule or growth in host; IMP:TAIR.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0030581; P:symbiont intracellular protein transport in host; IPI:TAIR.
DR Gene3D; 1.20.5.690; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032413; Arm_3.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR002652; Importin-a_IBB.
DR InterPro; IPR036975; Importin-a_IBB_sf.
DR InterPro; IPR024931; Importin_alpha.
DR Pfam; PF00514; Arm; 8.
DR Pfam; PF16186; Arm_3; 1.
DR Pfam; PF01749; IBB; 1.
DR PIRSF; PIRSF005673; Importin_alpha; 1.
DR SMART; SM00185; ARM; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 3.
DR PROSITE; PS51214; IBB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Protein transport; Reference proteome;
KW Repeat; Transport.
FT CHAIN 1..538
FT /note="Importin subunit alpha-4"
FT /id="PRO_0000431570"
FT DOMAIN 1..58
FT /note="IBB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00561"
FT REPEAT 112..152
FT /note="ARM 1"
FT /evidence="ECO:0000255"
FT REPEAT 155..194
FT /note="ARM 2"
FT /evidence="ECO:0000255"
FT REPEAT 197..237
FT /note="ARM 3"
FT /evidence="ECO:0000255"
FT REPEAT 239..278
FT /note="ARM 4"
FT /evidence="ECO:0000255"
FT REPEAT 281..320
FT /note="ARM 5"
FT /evidence="ECO:0000255"
FT REPEAT 323..363
FT /note="ARM 6"
FT /evidence="ECO:0000255"
FT REPEAT 366..405
FT /note="ARM 7"
FT /evidence="ECO:0000255"
FT REPEAT 409..448
FT /note="ARM 8"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..82
FT /note="Missing (in isoform 2)"
FT /id="VSP_057335"
FT CONFLICT 341..344
FT /note="LLTQ -> CDA (in Ref. 5; CAA74966)"
FT /evidence="ECO:0000305"
FT CONFLICT 521
FT /note="A -> G (in Ref. 5; CAA74966)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 59446 MW; C342198841B87CCA CRC64;
MSLRPSTRAE LRKKIYKTGV DADEARRRRE DNLVEIRKNK REDSLLKKRR EGMMLQQQLP
LGAGLDGPQT AAAVEKRLEG IPMMVQGVYS DDPQAQLEAT TQFRKLLSIE RSPPIDEVIK
AGVIPRFVEF LGRHDHPQLQ FEAAWALTNV ASGTSDHTRV VIEQGAVPIF VKLLTSASDD
VREQAVWALG NVAGDSPNCR NLVLNYGALE PLLAQLNENS KLSMLRNATW TLSNFCRGKP
PTPFEQVKPA LPILRQLIYL NDEEVLTDAC WALSYLSDGP NDKIQAVIEA GVCPRLVELL
GHQSPTVLIP ALRTVGNIVT GDDSQTQFII ESGVLPHLYN LLTQNHKKSI KKEACWTISN
ITAGNKLQIE AVVGAGIILP LVHLLQNAEF DIKKEAAWAI SNATSGGSHE QIQYLVTQGC
IKPLCDLLIC PDPRIVTVCL EGLENILKVG EADKEMGLNS GVNLYAQIIE ESDGLDKVEN
LQSHDNNEIY EKAVKILERY WAEEEEEQIL QDGGNDNSQQ AFNFGNNPAA PVGGFKFA
