ID   IMPA8_ARATH             Reviewed;         441 AA.
AC   Q9FJ92;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Importin subunit alpha-8 {ECO:0000305};
DE            Short=IMPa-8 {ECO:0000303|PubMed:18836040};
GN   Name=IMPA8 {ECO:0000303|PubMed:18836040};
GN   OrderedLocusNames=At5g52000 {ECO:0000312|Araport:AT5G52000};
GN   ORFNames=MSG15.8 {ECO:0000312|EMBL:BAB11048.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA   Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT   features of the regions of 1,013,767 bp covered by sixteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:297-308(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=18836040; DOI=10.1105/tpc.108.060467;
RA   Bhattacharjee S., Lee L.Y., Oltmanns H., Cao H., Gupta V., Cuperus J.,
RA   Gelvin S.B.;
RT   "IMPa-4, an Arabidopsis importin alpha isoform, is preferentially involved
RT   in agrobacterium-mediated plant transformation.";
RL   Plant Cell 20:2661-2680(2008).
CC   -!- FUNCTION: Binds to conventional NLS motifs and mediates nuclear protein
CC       import across the nuclear envelope. {ECO:0000250|UniProtKB:Q96321}.
CC   -!- SUBUNIT: Forms a complex with importin subunit beta-1.
CC       {ECO:0000250|UniProtKB:Q96321}.
CC   -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000250|UniProtKB:Q96321}.
CC   -!- SIMILARITY: Belongs to the importin alpha family. {ECO:0000305}.
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DR   EMBL; AB015478; BAB11048.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96157.1; -; Genomic_DNA.
DR   RefSeq; NP_200013.1; NM_124579.2.
DR   AlphaFoldDB; Q9FJ92; -.
DR   SMR; Q9FJ92; -.
DR   STRING; 3702.AT5G52000.1; -.
DR   PaxDb; Q9FJ92; -.
DR   PRIDE; Q9FJ92; -.
DR   ProteomicsDB; 248541; -.
DR   EnsemblPlants; AT5G52000.1; AT5G52000.1; AT5G52000.
DR   GeneID; 835275; -.
DR   Gramene; AT5G52000.1; AT5G52000.1; AT5G52000.
DR   KEGG; ath:AT5G52000; -.
DR   Araport; AT5G52000; -.
DR   TAIR; locus:2173123; AT5G52000.
DR   eggNOG; KOG0166; Eukaryota.
DR   HOGENOM; CLU_018084_6_0_1; -.
DR   InParanoid; Q9FJ92; -.
DR   OrthoDB; 1111872at2759; -.
DR   PhylomeDB; Q9FJ92; -.
DR   PRO; PR:Q9FJ92; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FJ92; baseline and differential.
DR   Genevisible; Q9FJ92; AT.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR   GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR   GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR   GO; GO:0048235; P:pollen sperm cell differentiation; IEP:TAIR.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR032413; Arm_3.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR021133; HEAT_type_2.
DR   InterPro; IPR024931; Importin_alpha.
DR   Pfam; PF00514; Arm; 5.
DR   Pfam; PF16186; Arm_3; 1.
DR   PIRSF; PIRSF005673; Importin_alpha; 1.
DR   SMART; SM00185; ARM; 8.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 3.
DR   PROSITE; PS50077; HEAT_REPEAT; 1.
PE   3: Inferred from homology;
KW   Nucleus; Protein transport; Reference proteome; Repeat; Transport.
FT   CHAIN           1..441
FT                   /note="Importin subunit alpha-8"
FT                   /id="PRO_0000431574"
FT   REPEAT          39..79
FT                   /note="ARM 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          80..118
FT                   /note="ARM 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          121..158
FT                   /note="ARM 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          160..199
FT                   /note="ARM 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          202..241
FT                   /note="ARM 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          244..284
FT                   /note="ARM 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          287..326
FT                   /note="ARM 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          330..370
FT                   /note="ARM 8"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   441 AA;  49338 MW;  1420A9F9F180794F CRC64;
     MAWKTEVNEV SDDIIDGLWS DDPPLQLESV TKIRRITSQR DISCVIRSGV VPRLVQLLKN
     QVFPKLQYEV AWALTNIAVD NPGVVVNNNA VPVLIQLIAS PKDYVREQAI WTLSNVAGHS
     IHYRDFVLNS GVLMPLLRLL YKDTTLRIAT WALRNLCRGK PHPAFDQVKP ALPALEILLH
     SHDEDVLKNA CMALCHLSEG SEDGIQSVIE AGFVPKLVQI LQLPSPVVLV PALLTIGAMT
     AGNHQQTQCV INSGALPIIS NMLTRNHENK IKKCACWVIS NITAGTKEQI QSVIDANLIP
     ILVNLAQDTD FYMKKEAVWA ISNMALNGSH DQIKYMAEQS CIKQLCDILV YSDERTTILK
     CLDGLENMLK AGEAEKNSED VNPYCLLIED AEGLEKISKL QMNKNDDIYE KAYKILVTNW
     FEEDDENNNN NVRCDDVDFQ V