IMPCT_CAEEL
ID IMPCT_CAEEL Reviewed; 257 AA.
AC Q9XWF4; Q7K779;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein IMPACT homolog;
GN Name=impt-1 {ECO:0000312|WormBase:Y52B11A.2a};
GN ORFNames=Y52B11A.2 {ECO:0000312|WormBase:Y52B11A.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Translational regulator that ensures constant high levels of
CC translation under amino acid starvation. Plays a role as a negative
CC regulator of the gcn-2 kinase activity; impairs gcn-1-mediated gcn-2
CC activation, and hence gcn-2-mediated eIF-2-alpha phosphorylation and
CC subsequent down-regulation of protein synthesis in amino acid-starved
CC cells. Plays a role in differentiation of neuronal cells by stimulating
CC neurite outgrowth. {ECO:0000250|UniProtKB:O55091}.
CC -!- SUBUNIT: Interacts with gcn-1; prevents the interaction of gcn-1 with
CC gcn-2 and inhibits gcn-2 kinase activity. Interaction with rpl-39; this
CC interaction occurs in a gcn-1-independent manner. Associates with
CC ribosomes; this interaction occurs in a gcn-1-independent manner.
CC Associates with actin; this interaction occurs in a gcn-1-independent
CC manner. {ECO:0000250|UniProtKB:O55091}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O55091}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:Y52B11A.2a};
CC IsoId=Q9XWF4-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y52B11A.2a};
CC IsoId=Q9XWF4-2; Sequence=VSP_033137, VSP_033138;
CC -!- SIMILARITY: Belongs to the IMPACT family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL032654; CAA21718.1; -; Genomic_DNA.
DR EMBL; AL032654; CAE54915.1; -; Genomic_DNA.
DR PIR; T27104; T27104.
DR RefSeq; NP_001021782.1; NM_001026611.2. [Q9XWF4-1]
DR RefSeq; NP_001021783.1; NM_001026612.2. [Q9XWF4-2]
DR AlphaFoldDB; Q9XWF4; -.
DR SMR; Q9XWF4; -.
DR BioGRID; 38410; 7.
DR STRING; 6239.Y52B11A.2a; -.
DR EPD; Q9XWF4; -.
DR PaxDb; Q9XWF4; -.
DR PeptideAtlas; Q9XWF4; -.
DR EnsemblMetazoa; Y52B11A.2a.1; Y52B11A.2a.1; WBGene00013122. [Q9XWF4-1]
DR EnsemblMetazoa; Y52B11A.2b.1; Y52B11A.2b.1; WBGene00013122. [Q9XWF4-2]
DR GeneID; 173001; -.
DR KEGG; cel:CELE_Y52B11A.2; -.
DR UCSC; Y52B11A.2a; c. elegans. [Q9XWF4-1]
DR CTD; 173001; -.
DR WormBase; Y52B11A.2a; CE20291; WBGene00013122; impt-1. [Q9XWF4-1]
DR WormBase; Y52B11A.2b; CE36243; WBGene00013122; impt-1. [Q9XWF4-2]
DR eggNOG; KOG3299; Eukaryota.
DR GeneTree; ENSGT00390000017571; -.
DR HOGENOM; CLU_045276_1_0_1; -.
DR InParanoid; Q9XWF4; -.
DR OMA; RHICNLA; -.
DR OrthoDB; 1400092at2759; -.
DR PhylomeDB; Q9XWF4; -.
DR PRO; PR:Q9XWF4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00013122; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0071468; P:cellular response to acidic pH; ISS:UniProtKB.
DR GO; GO:0034198; P:cellular response to amino acid starvation; ISS:UniProtKB.
DR GO; GO:0072755; P:cellular response to benomyl; ISS:UniProtKB.
DR GO; GO:0070301; P:cellular response to hydrogen peroxide; ISS:UniProtKB.
DR GO; GO:0060548; P:negative regulation of cell death; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0006446; P:regulation of translational initiation; IBA:GO_Central.
DR Gene3D; 3.10.110.10; -; 1.
DR Gene3D; 3.30.230.30; -; 1.
DR InterPro; IPR023582; Impact.
DR InterPro; IPR001498; Impact_N.
DR InterPro; IPR036956; Impact_N_sf.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR006575; RWD-domain.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR PANTHER; PTHR16301; PTHR16301; 1.
DR Pfam; PF05773; RWD; 1.
DR Pfam; PF01205; UPF0029; 1.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS50908; RWD; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Cytoplasm; Differentiation; Neurogenesis;
KW Reference proteome; Repressor; Stress response; Translation regulation.
FT CHAIN 1..257
FT /note="Protein IMPACT homolog"
FT /id="PRO_0000330858"
FT DOMAIN 9..102
FT /note="RWD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
FT VAR_SEQ 101
FT /note="D -> F (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_033137"
FT VAR_SEQ 102..257
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_033138"
SQ SEQUENCE 257 AA; 29599 MW; 739FCA5A563C2628 CRC64;
MSDEEQRIAE IESLASIFPD LLEETTDKQL VFKFDRDIGM TINLPEDYPS ISPPIFELSG
PYLRREQKEV LHNSLNDVYI ENIGFPVIFN WISLVQDFIR DLPEEVAEPS HVADDVVTPM
AEEIDDMNIR HGEVLTDRKS AFQAHLAEVR SKEDVDRVMR ILKSNTKICR ATHNITAYRY
MTEINGKPIH HHDCVDDGEF GASSKMLELM DRMKADNVMV VVSRWYGGIH LGPDRFRHIN
NLTRQILSDN NYGPKKS
