IMPE2_DROME
ID IMPE2_DROME Reviewed; 466 AA.
AC P29681; Q9VZN9;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=20-hydroxyecdysone protein;
DE Short=20-HE;
DE Flags: Precursor;
GN Name=ImpE2; Synonyms=IMP-E2; ORFNames=CG1934;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 444-454.
RX PubMed=2115480; DOI=10.1016/0012-1606(90)90084-v;
RA Paine-Saunders S., Fristrom D., Fristrom J.W.;
RT "The Drosophila IMP-E2 gene encodes an apically secreted protein expressed
RT during imaginal disc morphogenesis.";
RL Dev. Biol. 140:337-351(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Probably has an essential role in embryogenesis, induces
CC morphogenesis of imaginal disks, and may participate in multimolecular
CC aggregates.
CC -!- SUBCELLULAR LOCATION: Secreted. Note=Secreted from the apical cell
CC surface.
CC -!- DEVELOPMENTAL STAGE: Produced during mid embryogenesis, and imaginal
CC disk morphogenesis.
CC -!- DOMAIN: The regions 203-253, 256-333 and 335-377 are thought to contain
CC either alpha helical or beta pleated sheet motifs.
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DR EMBL; M55099; AAA63632.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF47780.1; -; Genomic_DNA.
DR EMBL; AY061270; AAL28818.1; -; mRNA.
DR PIR; A37351; A37351.
DR RefSeq; NP_476580.1; NM_057232.5.
DR AlphaFoldDB; P29681; -.
DR SMR; P29681; -.
DR BioGRID; 63925; 1.
DR DIP; DIP-22976N; -.
DR IntAct; P29681; 1.
DR STRING; 7227.FBpp0073000; -.
DR GlyGen; P29681; 2 sites.
DR PaxDb; P29681; -.
DR DNASU; 38432; -.
DR EnsemblMetazoa; FBtr0073141; FBpp0073000; FBgn0001254.
DR GeneID; 38432; -.
DR KEGG; dme:Dmel_CG1934; -.
DR CTD; 38432; -.
DR FlyBase; FBgn0001254; ImpE2.
DR VEuPathDB; VectorBase:FBgn0001254; -.
DR eggNOG; ENOG502SBRS; Eukaryota.
DR HOGENOM; CLU_615773_0_0_1; -.
DR InParanoid; P29681; -.
DR OMA; GTQFQNT; -.
DR OrthoDB; 1397818at2759; -.
DR PhylomeDB; P29681; -.
DR BioGRID-ORCS; 38432; 0 hits in 1 CRISPR screen.
DR ChiTaRS; ImpE2; fly.
DR GenomeRNAi; 38432; -.
DR PRO; PR:P29681; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0001254; Expressed in wing disc and 33 other tissues.
DR Genevisible; P29681; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005576; C:extracellular region; IDA:FlyBase.
DR GO; GO:0019898; C:extrinsic component of membrane; TAS:FlyBase.
DR GO; GO:0007561; P:imaginal disc eversion; TAS:FlyBase.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Reference proteome; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..466
FT /note="20-hydroxyecdysone protein"
FT /id="PRO_0000021510"
FT REPEAT 48..50
FT /note="1; approximate"
FT REPEAT 53..55
FT /note="2; approximate"
FT REPEAT 58..60
FT /note="3; approximate"
FT REPEAT 70..72
FT /note="4; approximate"
FT REPEAT 74..76
FT /note="5; approximate"
FT REPEAT 78..80
FT /note="6; approximate"
FT REPEAT 82..84
FT /note="7; approximate"
FT REPEAT 90..92
FT /note="8; approximate"
FT REPEAT 94..96
FT /note="9; approximate"
FT REPEAT 98..100
FT /note="10; approximate"
FT REPEAT 102..104
FT /note="11; approximate"
FT REPEAT 106..108
FT /note="12; approximate"
FT REPEAT 125..127
FT /note="13; approximate"
FT REPEAT 139..141
FT /note="14; approximate"
FT REPEAT 148..150
FT /note="15; approximate"
FT REPEAT 155..157
FT /note="16; approximate"
FT REGION 48..157
FT /note="16 X repeats"
FT REGION 55..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..435
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..159
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 294
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 352
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 466 AA; 49809 MW; A9FE40171C568BA9 CRC64;
MKPVALILVF LAISQARVLN LPKEAIDIPV AIVEDKEPPV ALSLVKEEVK AEEVKPEEVK
PIAQEEKAKD LKEEVKPEIK PEIKEQPKPD IKDEIKEDLK ADIKEELKEK IEEQINELPN
AKPLELKEKS LEAEEKPQEI KEEVQQPEIK KEATEIKEEP AQNILKSLPA EETVVVPAEE
LSPNPVEQEQ SENQDAAHPQ VRQATQATPT QQSTTQGNFV QQLIQNSPIG QFLNQFQPQP
AAAAAPAAAQ VQADDAAAAA PATPAPTVPG FLNPQAAITS AQQAVQNAAQ SAVNATTQAF
QGIQQFASNL GNQFQNTLSS LTGQQQQAVS TTPRPPGPIQ QFVNNVFGGN NNATAAAPPA
QQSGNPLQGI INFLGGNRPQ NAPAAAPATQ ATEKPAVDDK IDPANDEVGE FVPESDNELR
ASGENIDDSF EDAGVPSNEV IVVNDDAGEE GNAVQNHPVA TDAVAL
