IMPL2_DROME
ID IMPL2_DROME Reviewed; 267 AA.
AC Q09024; Q6NLN1; Q7KV52; Q95SU7; Q9VZF7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 4.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Neural/ectodermal development factor IMP-L2;
DE Flags: Precursor;
GN Name=ImpL2; ORFNames=CG15009;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B).
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=8306886; DOI=10.1242/dev.119.4.1237;
RA Garbe J.C., Yang E., Fristrom J.W.;
RT "IMP-L2: an essential secreted immunoglobulin family member implicated in
RT neural and ectodermal development in Drosophila.";
RL Development 119:1237-1250(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential developmental role during embryogenesis, in
CC particular the normal development of the nervous system. May be
CC involved in some aspect of cell adhesion.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=C;
CC IsoId=Q09024-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q09024-2; Sequence=VSP_010780;
CC Name=B;
CC IsoId=Q09024-3; Sequence=VSP_010779;
CC -!- TISSUE SPECIFICITY: Detected in several sites including the ventral
CC neuroectoderm, the tracheal pits, the pharynx and esophagus, and
CC specific neuronal cell bodies, where it is primarily expressed.
CC -!- DEVELOPMENTAL STAGE: First expressed at the cellular blastoderm stage
CC and continues to be expressed through subsequent development.
CC -!- INDUCTION: By 20-hydroxyecdysone.
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DR EMBL; L23066; AAB59251.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF47866.4; -; Genomic_DNA.
DR EMBL; AE014296; AAN11593.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11594.2; -; Genomic_DNA.
DR EMBL; AY060476; AAL25515.1; -; mRNA.
DR EMBL; BT012298; AAS77423.1; -; mRNA.
DR EMBL; BT012299; AAS77424.1; -; mRNA.
DR RefSeq; NP_001261409.1; NM_001274480.1. [Q09024-3]
DR RefSeq; NP_523921.3; NM_079197.5. [Q09024-2]
DR RefSeq; NP_728960.1; NM_168069.2. [Q09024-3]
DR RefSeq; NP_728961.2; NM_168070.2. [Q09024-1]
DR PDB; 4CBP; X-ray; 1.60 A; A/B=5-267.
DR PDB; 6FEY; X-ray; 3.48 A; A/B/C/D=26-267.
DR PDB; 6FF3; X-ray; 2.57 A; A=26-267.
DR PDBsum; 4CBP; -.
DR PDBsum; 6FEY; -.
DR PDBsum; 6FF3; -.
DR AlphaFoldDB; Q09024; -.
DR SASBDB; Q09024; -.
DR SMR; Q09024; -.
DR BioGRID; 63991; 18.
DR DIP; DIP-20664N; -.
DR IntAct; Q09024; 2.
DR STRING; 7227.FBpp0073106; -.
DR PaxDb; Q09024; -.
DR DNASU; 38513; -.
DR EnsemblMetazoa; FBtr0073248; FBpp0073104; FBgn0001257. [Q09024-2]
DR EnsemblMetazoa; FBtr0073249; FBpp0073105; FBgn0001257. [Q09024-3]
DR EnsemblMetazoa; FBtr0073250; FBpp0073106; FBgn0001257. [Q09024-1]
DR EnsemblMetazoa; FBtr0333569; FBpp0305746; FBgn0001257. [Q09024-3]
DR GeneID; 38513; -.
DR KEGG; dme:Dmel_CG15009; -.
DR CTD; 38513; -.
DR FlyBase; FBgn0001257; ImpL2.
DR VEuPathDB; VectorBase:FBgn0001257; -.
DR eggNOG; KOG3510; Eukaryota.
DR HOGENOM; CLU_072416_0_0_1; -.
DR InParanoid; Q09024; -.
DR OMA; VQWVHGS; -.
DR PhylomeDB; Q09024; -.
DR SignaLink; Q09024; -.
DR BioGRID-ORCS; 38513; 0 hits in 1 CRISPR screen.
DR ChiTaRS; ImpL2; fly.
DR GenomeRNAi; 38513; -.
DR PRO; PR:Q09024; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0001257; Expressed in eye disc (Drosophila) and 57 other tissues.
DR ExpressionAtlas; Q09024; baseline and differential.
DR Genevisible; Q09024; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0043559; F:insulin binding; IDA:FlyBase.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0046627; P:negative regulation of insulin receptor signaling pathway; IGI:FlyBase.
DR GO; GO:0010888; P:negative regulation of lipid storage; IMP:FlyBase.
DR GO; GO:0014067; P:negative regulation of phosphatidylinositol 3-kinase signaling; IGI:FlyBase.
DR GO; GO:0061965; P:positive regulation of entry into reproductive diapause; IMP:FlyBase.
DR GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; IDA:FlyBase.
DR GO; GO:0042594; P:response to starvation; IMP:FlyBase.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR Pfam; PF07679; I-set; 1.
DR SMART; SM00409; IG; 2.
DR SMART; SM00408; IGc2; 2.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell adhesion; Developmental protein;
KW Disulfide bond; Immunoglobulin domain; Reference proteome; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..267
FT /note="Neural/ectodermal development factor IMP-L2"
FT /id="PRO_0000014809"
FT DOMAIN 48..149
FT /note="Ig-like C2-type 1"
FT DOMAIN 174..260
FT /note="Ig-like C2-type 2"
FT DISULFID 80..139
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 195..244
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 1..4
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_010779"
FT VAR_SEQ 1..3
FT /note="MEA -> MQ (in isoform A)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_010780"
FT CONFLICT 177
FT /note="I -> V (in Ref. 1; AAB59251)"
FT /evidence="ECO:0000305"
FT HELIX 51..53
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 66..69
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 76..86
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 89..95
FT /evidence="ECO:0007829|PDB:4CBP"
FT HELIX 99..101
FT /evidence="ECO:0007829|PDB:6FF3"
FT STRAND 118..127
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 135..143
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 146..155
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 172..185
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 191..193
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 195..201
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 204..208
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 219..223
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 229..231
FT /evidence="ECO:0007829|PDB:4CBP"
FT HELIX 236..238
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 240..247
FT /evidence="ECO:0007829|PDB:4CBP"
FT STRAND 252..262
FT /evidence="ECO:0007829|PDB:4CBP"
SQ SEQUENCE 267 AA; 29895 MW; D4C4C72E4B1BDD3B CRC64;
MEAKMNLHVC ALALLLFGSI ATVRGRAVDL VDDSNDVDNS IEAEEEKPRN RAFEADWLKF
TKTPPTKLQQ ADGATIEIVC EMMGSQVPSI QWVVGHLPRS ELDDLDSNQV AEEAPSAIVR
VRSSHIIDHV LSEARTYTCV GRTGSKTIYA STVVHPPRSS RLTPEKTYPG AQKPRIIYTE
KTHLDLMGSN IQLPCRVHAR PRAEITWLNN ENKEIVQGHR HRVLANGDLL ISEIKWEDMG
NYKCIARNVV GKDTADTFVY PVLNEED
