ID   IMPX_FUSN2              Reviewed;         299 AA.
AC   D5RAW5;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Riboflavin transporter ImpX {ECO:0000305};
GN   Name=impX {ECO:0000303|PubMed:25938806};
GN   ORFNames=HMPREF0397_0350 {ECO:0000312|EMBL:EFG96032.1};
OS   Fusobacterium nucleatum subsp. nucleatum (strain ATCC 23726 / VPI 4351).
OC   Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX   NCBI_TaxID=525283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23726 / VPI 4351;
RA   Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., Chaboub L.,
RA   Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., Fowler G.,
RA   Francisco L., Fu Q., Gubbala S., Hale W., Han Y., Hemphill L.,
RA   Highlander S.K., Hirani K., Hogues M., Jackson L., Jakkamsetti A.,
RA   Javaid M., Jiang H., Korchina V., Kovar C., Lara F., Lee S., Mata R.,
RA   Mathew T., Moen C., Morales K., Munidasa M., Nazareth L., Ngo R.,
RA   Nguyen L., Okwuonu G., Ongeri F., Patil S., Petrosino J., Pham C., Pham P.,
RA   Pu L.-L., Puazo M., Raj R., Reid J., Rouhana J., Saada N., Shang Y.,
RA   Simmons D., Thornton R., Warren J., Weissenberger G., Zhang J., Zhang L.,
RA   Zhou C., Zhu D., Muzny D., Worley K., Gibbs R.;
RL   Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION AS A TRANSPORTER, AND INDUCTION.
RC   STRAIN=ATCC 23726 / VPI 4351;
RX   PubMed=25938806; DOI=10.1371/journal.pone.0126124;
RA   Gutierrez-Preciado A., Torres A.G., Merino E., Bonomi H.R., Goldbaum F.A.,
RA   Garcia-Angulo V.A.;
RT   "Extensive identification of bacterial riboflavin transporters and their
RT   distribution across bacterial species.";
RL   PLoS ONE 10:E0126124-E0126124(2015).
CC   -!- FUNCTION: Transports riboflavin into the cell.
CC       {ECO:0000269|PubMed:25938806}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is probably regulated by riboflavin, via an FMN
CC       riboswitch. {ECO:0000269|PubMed:25938806}.
CC   -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR   EMBL; ADVK01000012; EFG96032.1; -; Genomic_DNA.
DR   RefSeq; WP_005902102.1; NZ_CP028109.1.
DR   AlphaFoldDB; D5RAW5; -.
DR   SMR; D5RAW5; -.
DR   TCDB; 2.A.7.3.71; the drug/metabolite transporter (dmt) superfamily.
DR   EnsemblBacteria; EFG96032; EFG96032; HMPREF0397_0350.
DR   Proteomes; UP000003643; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Repeat; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..299
FT                   /note="Riboflavin transporter ImpX"
FT                   /id="PRO_0000438276"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        253..273
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          6..144
FT                   /note="EamA 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          162..294
FT                   /note="EamA 2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   299 AA;  33263 MW;  F3ED1968139E4342 CRC64;
     MDNHIKGALL VCLAATMWGF DGIALTPRLF SLHVPFVVFI LHLLPLILMS ILFGKEEVKN
     IKKLQKNDLF FFFCVALFGG CLGTLCIVKA LFLVNFKHLT VVTLLQKLQP IFAIILARLL
     LKEKLKRAYL FWGFLALLGG YLLTFEFHLP EFVSSDNLLP ASLYSLLAAF SFGSATVFGK
     RILKSASFRT ALYLRYLMTS CIMFVIVTFT SGFGDFLVAT AGNWLIFVII ALTTGSGAIL
     LYYFGLRYIT AKVATMCELC FPISSVVFDY LINGNVLSPV QIASAILMII SIIKISKLN