ID   IMQA_ASPFN              Reviewed;          99 AA.
AC   B8NI18;
DT   20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Imizoquin biosynthesis cluster protein A {ECO:0000303|PubMed:29182847};
GN   Name=imqA {ECO:0000303|PubMed:29182847}; ORFNames=AFLA_064230;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   INDUCTION, FUNCTION, AND PATHWAY.
RX   PubMed=29182847; DOI=10.1021/acschembio.7b00731;
RA   Khalid S., Baccile J.A., Spraker J.E., Tannous J., Imran M.,
RA   Schroeder F.C., Keller N.P.;
RT   "NRPS-derived isoquinolines and lipopetides mediate antagonism between
RT   plant pathogenic fungi and bacteria.";
RL   ACS Chem. Biol. 13:171-179(2018).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       imizoquins A to D, tripeptide-derived alkaloids that serve a protective
CC       role against oxidative stress that are essential for normal germination
CC       (PubMed:29182847). ImqB is a canonical three-module NRPS that assembles
CC       the tripeptide backbone of the imizoquins via condensation of Trp, Tyr,
CC       and Leu-derived precursors (PubMed:29182847). N-methylation by imqF and
CC       phenol oxidation by imqC, followed by cyclization via the FAD-dependent
CC       oxidase imqH carry out the three-step transformation of L-tyrosine into
CC       tetrahydroisoquinoline (PubMed:29182847). Importantly, this sequence
CC       requires the presence of a free amine in the tyrosine moiety,
CC       indicating that isoquinoline formation occurs prior to peptide bond
CC       formation (PubMed:29182847). The imidazolidin-4-one ring of imizoquins
CC       could form following additional oxidation of the methyl-derived
CC       bridgehead carbon by imqH (PubMed:29182847). Lastly, O-methylation by
CC       imqG and leucine hydroxylation by imqE complete biosynthesis of the
CC       imizoquins (PubMed:29182847). {ECO:0000269|PubMed:29182847}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:29182847}.
CC   -!- INDUCTION: Expression is down-regulated by ralstonins, lipopeptides
CC       produced by the plant pathogenic bacteria Ralstonia solanacearum
CC       (PubMed:29182847). Expression is positively regulated by the imizoquins
CC       cluster-specific transcription regulator imqK (PubMed:29182847).
CC       {ECO:0000269|PubMed:29182847}.
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DR   EMBL; EQ963479; EED49602.1; -; Genomic_DNA.
DR   RefSeq; XP_002379983.1; XM_002379942.1.
DR   AlphaFoldDB; B8NI18; -.
DR   EnsemblFungi; EED49602; EED49602; AFLA_064230.
DR   VEuPathDB; FungiDB:AFLA_064230; -.
DR   HOGENOM; CLU_2319899_0_0_1; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
PE   2: Evidence at transcript level;
FT   CHAIN           1..99
FT                   /note="Imizoquin biosynthesis cluster protein A"
FT                   /id="PRO_0000444545"
SQ   SEQUENCE   99 AA;  11054 MW;  5B47F3233FC0E66B CRC64;
     MVDSVFPAPS LVDETFLAPS SSETNDTAMA ADGTLAKMNM FQFNSMLKEY SPPRFEDLFQ
     DLDASKAGWE HYRPRLVSEV DLIDAEHYSL FSANTVSPL