ID   IMSP5_RUDPH             Reviewed;         135 AA.
AC   P86986;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=Insoluble matrix shell protein 5 {ECO:0000303|PubMed:21221694};
DE            Short=IMSP5 {ECO:0000303|PubMed:21221694};
DE   Flags: Precursor;
OS   Ruditapes philippinarum (Japanese littleneck clam) (Venerupis
OS   philippinarum).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Heteroconchia; Euheterodonta; Imparidentia; Neoheterodontei;
OC   Venerida; Veneroidea; Veneridae; Ruditapes.
OX   NCBI_TaxID=129788;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Beck A.;
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 32-40.
RC   TISSUE=Shell {ECO:0000269|PubMed:21221694};
RX   PubMed=21221694; DOI=10.1007/s10126-010-9357-0;
RA   Marie B., Trinkler N., Zanella-Cleon I., Guichard N., Becchi M.,
RA   Paillard C., Marin F.;
RT   "Proteomic identification of novel proteins from the calcifying shell
RT   matrix of the manila clam Venerupis Philippinarum.";
RL   Mar. Biotechnol. 13:955-962(2011).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21221694}.
CC   -!- TISSUE SPECIFICITY: Component of the acid-insoluble organic matrix of
CC       the calcified shell. {ECO:0000269|PubMed:21221694}.
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DR   EMBL; AM874357; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P86986; -.
DR   SMR; P86986; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13202; EF-hand_5; 3.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Metal-binding; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..135
FT                   /note="Insoluble matrix shell protein 5"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000413029"
FT   DOMAIN          21..56
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          93..128
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         34
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         36
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         38
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         40
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         45
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         106
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         108
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         110
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         112
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         117
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   135 AA;  15606 MW;  C561A06694EAB0EB CRC64;
     MILVVTLACL IAVVCCQCPT TDQGQISKVF KAYDIDGNNK ISRVEGTMVF RDADLNRDGA
     LDNNEFSSEW AFYHNDYYSP FFNVADRNHN GRIEFVEGNQ GFDHFDKNRD NEISSWEFTQ
     TWMETVRPSS RPIDF