IMT3_SCHPO
ID IMT3_SCHPO Reviewed; 356 AA.
AC Q10323; Q9US96;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Inositol phosphoceramide mannosyltransferase 3;
DE EC=2.4.-.-;
DE AltName: Full=IPC mannosyltransferase 3;
GN ORFNames=SPAC17G8.11c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-83, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307; SER-353 AND SER-355, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
RN [5]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=20388730; DOI=10.1242/jcs.059139;
RA Nakase M., Tani M., Morita T., Kitamoto H.K., Kashiwazaki J., Nakamura T.,
RA Hosomi A., Tanaka N., Takegawa K.;
RT "Mannosylinositol phosphorylceramide is a major sphingolipid component and
RT is required for proper localization of plasma-membrane proteins in
RT Schizosaccharomyces pombe.";
RL J. Cell Sci. 123:1578-1587(2010).
CC -!- FUNCTION: With imt1 and imt2, is required for the synthesis of
CC mannosylinositol phosphoceramide (MIPC). Catalyzes the addition of
CC mannosyl to inositol phosphoceramide (IPC). MIPC is essential for cell
CC morphology, cell-surface distribution of ergosterol, localization for
CC plasma-membrane transporters, and lipid-raft-mediated endocytosis of
CC plasma membrane proteins to the vacuole. {ECO:0000269|PubMed:20388730}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-
CC pass membrane protein. Golgi apparatus, trans-Golgi network membrane;
CC Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 32 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329670; CAA93694.1; -; Genomic_DNA.
DR EMBL; AB027932; BAA87236.1; -; Genomic_DNA.
DR PIR; T37863; T37863.
DR RefSeq; NP_593734.1; NM_001019165.2.
DR AlphaFoldDB; Q10323; -.
DR BioGRID; 278760; 1.
DR STRING; 4896.SPAC17G8.11c.1; -.
DR CAZy; GT32; Glycosyltransferase Family 32.
DR iPTMnet; Q10323; -.
DR MaxQB; Q10323; -.
DR PaxDb; Q10323; -.
DR PRIDE; Q10323; -.
DR EnsemblFungi; SPAC17G8.11c.1; SPAC17G8.11c.1:pep; SPAC17G8.11c.
DR PomBase; SPAC17G8.11c; -.
DR VEuPathDB; FungiDB:SPAC17G8.11c; -.
DR eggNOG; ENOG502QS3D; Eukaryota.
DR HOGENOM; CLU_036369_3_0_1; -.
DR InParanoid; Q10323; -.
DR OMA; NKPQLIP; -.
DR PhylomeDB; Q10323; -.
DR PRO; PR:Q10323; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0033106; C:cis-Golgi network membrane; IDA:PomBase.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032588; C:trans-Golgi network membrane; IDA:PomBase.
DR GO; GO:0000030; F:mannosyltransferase activity; ISO:PomBase.
DR GO; GO:0051999; P:mannosyl-inositol phosphorylceramide biosynthetic process; IGI:PomBase.
DR InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF04488; Gly_transf_sug; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Golgi apparatus;
KW Membrane; Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..356
FT /note="Inositol phosphoceramide mannosyltransferase 3"
FT /id="PRO_0000116591"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 353
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 355
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 356 AA; 42197 MW; DB1992BD81AECDCD CRC64;
MNKILFYFFF FLTLILSATV YLFGGPMMLF FINYKTDLLK VDDVYNHEIY SNQSAAIPKI
IHQTWKTNEI PEKWVGAQKS CIDLHPDYEY VLWTDESMRE FIATDYPWFL TQYDSYPYNI
ERADVVRYFI LYKYGGIYLD LDVGCNRTLD PLLHYPAWVR RTSPSGISNN VMGFAKGHPF
LLQVVRNLPR FAFNYHFPYL TVMYSTGPLF LSIIWSAWRK LPDAEAWHHI WVMVPELYEK
SHHAFFEIYE GSSWHDSDAG FVFYMLHHWA IFTFLGFLTF FIVVYFIFGY ALKPAARVSR
TGRRVFSSPF SKTSPSRWKI FHRFTSSNEK YDQTRSDSLP FMSDYDLESQ TQSHSP
