ID   IN22_MAIZE              Reviewed;         306 AA.
AC   P49249;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=IN2-2 protein;
GN   Name=IN2-2; Synonyms=SAF2;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Missouri 17;
RX   PubMed=1912492; DOI=10.1007/bf00037053;
RA   Hershey H.P., Stoner T.D.;
RT   "Isolation and characterization of cDNA clones for RNA species induced by
RT   substituted benzenesulfonamides in corn.";
RL   Plant Mol. Biol. 17:679-690(1991).
CC   -!- TISSUE SPECIFICITY: Leaves and roots.
CC   -!- DEVELOPMENTAL STAGE: It appears in roots within 30 minutes of
CC       induction, maximum levels are reached by 6 hours, and remains constant
CC       for 2 days. In leaves it is seen 9 hours after induction, and reaches
CC       maximum levels after 24 hours.
CC   -!- INDUCTION: By N-(aminocarbonyl)-2-chlorobenzenesulfonamide (2-CBSU).
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. Aldo/keto
CC       reductase 2 subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P49249; -.
DR   SMR; P49249; -.
DR   PaxDb; P49249; -.
DR   PRIDE; P49249; -.
DR   MaizeGDB; 121988; -.
DR   eggNOG; KOG1575; Eukaryota.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; P49249; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004033; F:aldo-keto reductase (NADP) activity; IBA:GO_Central.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
PE   2: Evidence at transcript level;
KW   NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..306
FT                   /note="IN2-2 protein"
FT                   /id="PRO_0000070381"
FT   REGION          272..306
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        64
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         131
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         210..220
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            90
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   306 AA;  33829 MW;  2E94674C7FDE3CBD CRC64;
     MAAALVSVPR IKLGSQGLEV SAQGLGCMGM SAFYGPPKPE SEMIKLIHHA VDAGVTFLDT
     SDVYGPHTNE VLLGKALQGG VREKVELATK FGVSFADGKR EIHGDPAYVR TACEGSFKRL
     GVDCIDLYYQ HRIDKRVPIE VTIGELKKLV EEGKIKYIGL SEASASTIRR AHAVHPITAV
     QLEWSLWSRD AEEDIIPTCR ELGIGIVAYS PLGRGFFSSG AKLVDSLSEQ DFRKHMPRFQ
     PENLDKNAQI FERVRRDGST ERMHAITARV GLGSPPRKRR LPHTWHNKNR QLQPERGGTV
     CEAYTG