INCA1_HUMAN
ID INCA1_HUMAN Reviewed; 236 AA.
AC Q0VD86; Q6J273; Q6PKN9;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Protein INCA1;
DE AltName: Full=Inhibitor of CDK interacting with cyclin A1;
GN Name=INCA1; ORFNames=HSD45;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CCNA1,
RP IDENTIFICATION IN A COMPLEX WITH CCNA1 AND CDK2, PHOSPHORYLATION AT SER-23;
RP THR-182; SER-191 AND SER-194, MUTAGENESIS OF SER-23; THR-182; SER-191 AND
RP SER-194, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=15159402; DOI=10.1074/jbc.m401708200;
RA Diederichs S., Baeumer N., Ji P., Metzelder S.K., Idos G.E., Cauvet T.,
RA Wang W., Moeller M., Pierschalski S., Gromoll J., Schrader M.G.,
RA Koeffler H.P., Berdel W.E., Serve H., Mueller-Tidow C.;
RT "Identification of interaction partners and substrates of the cyclin A1-
RT CDK2 complex.";
RL J. Biol. Chem. 279:33727-33741(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RA Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.;
RT "A new spermatogenesis-related gene.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH ZNF16.
RX PubMed=21874239; DOI=10.3892/mmr.2011.564;
RA Li X.B., Chen J., Deng M.J., Wang F., Du Z.W., Zhang J.W.;
RT "Zinc finger protein HZF1 promotes K562 cell proliferation by interacting
RT with and inhibiting INCA1.";
RL Mol. Med. Report. 4:1131-1137(2011).
RN [5]
RP INTERACTION WITH SPACA9, AND SUBCELLULAR LOCATION.
RX PubMed=18756329; DOI=10.1139/o08-072;
RA Chen X., Hu T., Liang G., Yang M., Zong S., Miao S., Koide S.S., Wang L.;
RT "A novel testis protein, RSB-66, interacting with INCA1 (inhibitor of Cdk
RT interacting with cyclin A1).";
RL Biochem. Cell Biol. 86:345-351(2008).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH CCNA1; CCNA2; CCNB1 AND
RP CCNE1, INTERACTION WITH THE CCNA1/CDK2 COMPLEX, AND INDUCTION.
RX PubMed=21540187; DOI=10.1074/jbc.m110.203471;
RA Baeumer N., Tickenbrock L., Tschanter P., Lohmeyer L., Diederichs S.,
RA Baeumer S., Skryabin B.V., Zhang F., Agrawal-Singh S., Koehler G.,
RA Berdel W.E., Serve H., Koschmieder S., Mueller-Tidow C.;
RT "Inhibitor of cyclin-dependent kinase (CDK) interacting with cyclin A1
RT (INCA1) regulates proliferation and is repressed by oncogenic signaling.";
RL J. Biol. Chem. 286:28210-28222(2011).
RN [7]
RP INTERACTION WITH ING5; DAZAP2; RNF26; USP15; SPOUT1; DPH7; TRIM26 AND
RP RAB5C.
RX PubMed=21750715; DOI=10.1371/journal.pone.0021505;
RA Zhang F., Baeumer N., Rode M., Ji P., Zhang T., Berdel W.E.,
RA Mueller-Tidow C.;
RT "The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor
RT for its antiproliferative effects.";
RL PLoS ONE 6:E21505-E21505(2011).
CC -!- FUNCTION: Binds to CDK2-bound cyclins and inhibits the kinase activity
CC of CDK2; binding to cyclins is critical for its function as CDK
CC inhibitor (PubMed:21540187). Inhibits cell growth and cell
CC proliferation and may play a role in cell cycle control (By
CC similarity). Required for ING5-mediated regulation of S-phase
CC progression, enhancement of Fas-induced apoptosis and inhibition of
CC cell growth (By similarity). {ECO:0000250|UniProtKB:Q6PKN7,
CC ECO:0000269|PubMed:21540187}.
CC -!- SUBUNIT: Interacts with CCNA1 (PubMed:21540187, PubMed:15159402).
CC Interacts with CCNA2, CCNB1 and CCNE1 (PubMed:21540187). Found in a
CC complex with CCNA1 and CDK2 (PubMed:15159402). Interacts with ZNF16;
CC the interaction inhibits INCA1 activity and induces the cell cycle
CC process (PubMed:21874239). Interacts with SPACA9 (PubMed:18756329).
CC Interacts with the CCNA1/CDK2 complex (PubMed:21540187). Interacts with
CC ING5, DAZAP2, RNF26, USP15, SPOUT1, DPH7, TRIM26 and RAB5C
CC (PubMed:21750715). {ECO:0000269|PubMed:15159402,
CC ECO:0000269|PubMed:18756329, ECO:0000269|PubMed:21540187,
CC ECO:0000269|PubMed:21750715, ECO:0000269|PubMed:21874239}.
CC -!- INTERACTION:
CC Q0VD86; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-6509505, EBI-11096309;
CC Q0VD86; P68133: ACTA1; NbExp=3; IntAct=EBI-6509505, EBI-367510;
CC Q0VD86; Q96HD9: ACY3; NbExp=5; IntAct=EBI-6509505, EBI-3916242;
CC Q0VD86; Q9ULX6: AKAP8L; NbExp=3; IntAct=EBI-6509505, EBI-357530;
CC Q0VD86; O15084: ANKRD28; NbExp=3; IntAct=EBI-6509505, EBI-359567;
CC Q0VD86; Q9H6L4: ARMC7; NbExp=3; IntAct=EBI-6509505, EBI-742909;
CC Q0VD86; Q9BZE9: ASPSCR1; NbExp=3; IntAct=EBI-6509505, EBI-1993677;
CC Q0VD86; Q9H1Y0: ATG5; NbExp=3; IntAct=EBI-6509505, EBI-1047414;
CC Q0VD86; Q86V38: ATN1; NbExp=3; IntAct=EBI-6509505, EBI-11954292;
CC Q0VD86; P13637: ATP1A3; NbExp=3; IntAct=EBI-6509505, EBI-948169;
CC Q0VD86; Q8N8Y2: ATP6V0D2; NbExp=3; IntAct=EBI-6509505, EBI-3923949;
CC Q0VD86; Q8N5M1: ATPAF2; NbExp=3; IntAct=EBI-6509505, EBI-1166928;
CC Q0VD86; O95817: BAG3; NbExp=3; IntAct=EBI-6509505, EBI-747185;
CC Q0VD86; Q9H503-2: BANF2; NbExp=3; IntAct=EBI-6509505, EBI-11977289;
CC Q0VD86; Q53S33: BOLA3; NbExp=3; IntAct=EBI-6509505, EBI-12086950;
CC Q0VD86; Q9NP55: BPIFA1; NbExp=3; IntAct=EBI-6509505, EBI-953896;
CC Q0VD86; Q5SWW7: C10orf55; NbExp=3; IntAct=EBI-6509505, EBI-12809220;
CC Q0VD86; Q5BKX5-3: C19orf54; NbExp=3; IntAct=EBI-6509505, EBI-11976299;
CC Q0VD86; Q9BV19: C1orf50; NbExp=3; IntAct=EBI-6509505, EBI-2874661;
CC Q0VD86; Q6P5X5: C22orf39; NbExp=3; IntAct=EBI-6509505, EBI-7317823;
CC Q0VD86; Q9NP86: CABP5; NbExp=10; IntAct=EBI-6509505, EBI-10311131;
CC Q0VD86; Q6UXH8-3: CCBE1; NbExp=3; IntAct=EBI-6509505, EBI-12013534;
CC Q0VD86; Q2TAC2-2: CCDC57; NbExp=3; IntAct=EBI-6509505, EBI-10961624;
CC Q0VD86; A2IDD5-4: CCDC78; NbExp=3; IntAct=EBI-6509505, EBI-18205352;
CC Q0VD86; P32320: CDA; NbExp=3; IntAct=EBI-6509505, EBI-9250559;
CC Q0VD86; Q9UJX2: CDC23; NbExp=3; IntAct=EBI-6509505, EBI-396137;
CC Q0VD86; Q07002: CDK18; NbExp=3; IntAct=EBI-6509505, EBI-746238;
CC Q0VD86; P11802: CDK4; NbExp=3; IntAct=EBI-6509505, EBI-295644;
CC Q0VD86; P38936: CDKN1A; NbExp=3; IntAct=EBI-6509505, EBI-375077;
CC Q0VD86; P42772: CDKN2B; NbExp=3; IntAct=EBI-6509505, EBI-711280;
CC Q0VD86; P55273: CDKN2D; NbExp=9; IntAct=EBI-6509505, EBI-745859;
CC Q0VD86; Q9P209: CEP72; NbExp=3; IntAct=EBI-6509505, EBI-739498;
CC Q0VD86; Q9Y6H1: CHCHD2; NbExp=3; IntAct=EBI-6509505, EBI-2321769;
CC Q0VD86; Q9BSY4: CHCHD5; NbExp=3; IntAct=EBI-6509505, EBI-11521409;
CC Q0VD86; Q9H2X0: CHRD; NbExp=3; IntAct=EBI-6509505, EBI-947551;
CC Q0VD86; Q9BW66: CINP; NbExp=3; IntAct=EBI-6509505, EBI-739784;
CC Q0VD86; P61024: CKS1B; NbExp=3; IntAct=EBI-6509505, EBI-456371;
CC Q0VD86; O95833: CLIC3; NbExp=3; IntAct=EBI-6509505, EBI-10192241;
CC Q0VD86; P49761: CLK3; NbExp=3; IntAct=EBI-6509505, EBI-745579;
CC Q0VD86; Q96A83-2: COL26A1; NbExp=3; IntAct=EBI-6509505, EBI-21553822;
CC Q0VD86; P27658: COL8A1; NbExp=9; IntAct=EBI-6509505, EBI-747133;
CC Q0VD86; Q10570: CPSF1; NbExp=3; IntAct=EBI-6509505, EBI-347859;
CC Q0VD86; P02489: CRYAA; NbExp=3; IntAct=EBI-6509505, EBI-6875961;
CC Q0VD86; Q9Y534: CSDC2; NbExp=3; IntAct=EBI-6509505, EBI-1763657;
CC Q0VD86; P33240: CSTF2; NbExp=3; IntAct=EBI-6509505, EBI-711360;
CC Q0VD86; Q9NSA3: CTNNBIP1; NbExp=3; IntAct=EBI-6509505, EBI-747082;
CC Q0VD86; Q8WZ74: CTTNBP2; NbExp=3; IntAct=EBI-6509505, EBI-1774260;
CC Q0VD86; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-6509505, EBI-3867333;
CC Q0VD86; Q15038: DAZAP2; NbExp=2; IntAct=EBI-6509505, EBI-724310;
CC Q0VD86; Q9H4E7: DEF6; NbExp=3; IntAct=EBI-6509505, EBI-745369;
CC Q0VD86; Q9NQL9: DMRT3; NbExp=6; IntAct=EBI-6509505, EBI-9679045;
CC Q0VD86; Q96MA1: DMRTB1; NbExp=3; IntAct=EBI-6509505, EBI-954466;
CC Q0VD86; Q8TEW6: DOK4; NbExp=3; IntAct=EBI-6509505, EBI-6918542;
CC Q0VD86; Q9BTV6: DPH7; NbExp=2; IntAct=EBI-6509505, EBI-11059920;
CC Q0VD86; Q9UII6: DUSP13; NbExp=3; IntAct=EBI-6509505, EBI-749800;
CC Q0VD86; O95967: EFEMP2; NbExp=3; IntAct=EBI-6509505, EBI-743414;
CC Q0VD86; P41091: EIF2S3; NbExp=3; IntAct=EBI-6509505, EBI-1054228;
CC Q0VD86; P50402: EMD; NbExp=3; IntAct=EBI-6509505, EBI-489887;
CC Q0VD86; O15197-2: EPHB6; NbExp=8; IntAct=EBI-6509505, EBI-10182490;
CC Q0VD86; O75460-2: ERN1; NbExp=3; IntAct=EBI-6509505, EBI-25852368;
CC Q0VD86; Q96B26: EXOSC8; NbExp=3; IntAct=EBI-6509505, EBI-371922;
CC Q0VD86; Q6NZ36-4: FAAP20; NbExp=3; IntAct=EBI-6509505, EBI-12013806;
CC Q0VD86; Q9NVQ4-2: FAIM; NbExp=3; IntAct=EBI-6509505, EBI-12039347;
CC Q0VD86; Q9BSK4: FEM1A; NbExp=3; IntAct=EBI-6509505, EBI-2515349;
CC Q0VD86; O43320: FGF16; NbExp=3; IntAct=EBI-6509505, EBI-11479104;
CC Q0VD86; Q14192: FHL2; NbExp=5; IntAct=EBI-6509505, EBI-701903;
CC Q0VD86; Q0VDC6: FKBP1A; NbExp=3; IntAct=EBI-6509505, EBI-10226858;
CC Q0VD86; O43559: FRS3; NbExp=3; IntAct=EBI-6509505, EBI-725515;
CC Q0VD86; P55040: GEM; NbExp=3; IntAct=EBI-6509505, EBI-744104;
CC Q0VD86; O14893: GEMIN2; NbExp=3; IntAct=EBI-6509505, EBI-443648;
CC Q0VD86; P62993: GRB2; NbExp=4; IntAct=EBI-6509505, EBI-401755;
CC Q0VD86; A8MXD5: GRXCR1; NbExp=3; IntAct=EBI-6509505, EBI-5235612;
CC Q0VD86; Q9H4Y5: GSTO2; NbExp=3; IntAct=EBI-6509505, EBI-10194609;
CC Q0VD86; Q6ZYL4: GTF2H5; NbExp=3; IntAct=EBI-6509505, EBI-6380438;
CC Q0VD86; Q969Y2: GTPBP3; NbExp=3; IntAct=EBI-6509505, EBI-740290;
CC Q0VD86; P13807: GYS1; NbExp=3; IntAct=EBI-6509505, EBI-740553;
CC Q0VD86; Q96CS2: HAUS1; NbExp=3; IntAct=EBI-6509505, EBI-2514791;
CC Q0VD86; P08631-2: HCK; NbExp=3; IntAct=EBI-6509505, EBI-9834454;
CC Q0VD86; P56524-2: HDAC4; NbExp=3; IntAct=EBI-6509505, EBI-11953488;
CC Q0VD86; Q9Y5R4: HEMK1; NbExp=5; IntAct=EBI-6509505, EBI-10329202;
CC Q0VD86; Q5T8I9: HENMT1; NbExp=3; IntAct=EBI-6509505, EBI-9675710;
CC Q0VD86; P52597: HNRNPF; NbExp=3; IntAct=EBI-6509505, EBI-352986;
CC Q0VD86; Q9NSC5: HOMER3; NbExp=3; IntAct=EBI-6509505, EBI-748420;
CC Q0VD86; P49639: HOXA1; NbExp=3; IntAct=EBI-6509505, EBI-740785;
CC Q0VD86; O43593: HR; NbExp=3; IntAct=EBI-6509505, EBI-2880706;
CC Q0VD86; Q9BPX1: HSD17B14; NbExp=3; IntAct=EBI-6509505, EBI-742664;
CC Q0VD86; P54652: HSPA2; NbExp=3; IntAct=EBI-6509505, EBI-356991;
CC Q0VD86; P10809: HSPD1; NbExp=3; IntAct=EBI-6509505, EBI-352528;
CC Q0VD86; Q9UBH0: IL36RN; NbExp=3; IntAct=EBI-6509505, EBI-465156;
CC Q0VD86; Q12905: ILF2; NbExp=3; IntAct=EBI-6509505, EBI-357925;
CC Q0VD86; Q0VD86: INCA1; NbExp=3; IntAct=EBI-6509505, EBI-6509505;
CC Q0VD86; Q8WYH8: ING5; NbExp=4; IntAct=EBI-6509505, EBI-488533;
CC Q0VD86; Q96MP8-2: KCTD7; NbExp=3; IntAct=EBI-6509505, EBI-11954971;
CC Q0VD86; Q7L273: KCTD9; NbExp=3; IntAct=EBI-6509505, EBI-4397613;
CC Q0VD86; Q5T5P2-6: KIAA1217; NbExp=3; IntAct=EBI-6509505, EBI-10188326;
CC Q0VD86; Q99706: KIR2DL4; NbExp=3; IntAct=EBI-6509505, EBI-10294579;
CC Q0VD86; O14901: KLF11; NbExp=3; IntAct=EBI-6509505, EBI-948266;
CC Q0VD86; Q53HC5: KLHL26; NbExp=3; IntAct=EBI-6509505, EBI-724915;
CC Q0VD86; Q9P2K6: KLHL42; NbExp=3; IntAct=EBI-6509505, EBI-739890;
CC Q0VD86; Q92876: KLK6; NbExp=3; IntAct=EBI-6509505, EBI-2432309;
CC Q0VD86; Q14525: KRT33B; NbExp=3; IntAct=EBI-6509505, EBI-1049638;
CC Q0VD86; P13647: KRT5; NbExp=3; IntAct=EBI-6509505, EBI-702187;
CC Q0VD86; O95678: KRT75; NbExp=3; IntAct=EBI-6509505, EBI-2949715;
CC Q0VD86; Q3LI76: KRTAP15-1; NbExp=3; IntAct=EBI-6509505, EBI-11992140;
CC Q0VD86; Q8IUB9: KRTAP19-1; NbExp=3; IntAct=EBI-6509505, EBI-12811111;
CC Q0VD86; Q3LI72: KRTAP19-5; NbExp=5; IntAct=EBI-6509505, EBI-1048945;
CC Q0VD86; Q3LI70: KRTAP19-6; NbExp=3; IntAct=EBI-6509505, EBI-12805508;
CC Q0VD86; Q6L8G9: KRTAP5-6; NbExp=3; IntAct=EBI-6509505, EBI-10250562;
CC Q0VD86; Q3LI66: KRTAP6-2; NbExp=5; IntAct=EBI-6509505, EBI-11962084;
CC Q0VD86; Q9BYQ3: KRTAP9-3; NbExp=3; IntAct=EBI-6509505, EBI-1043191;
CC Q0VD86; Q96PV6: LENG8; NbExp=3; IntAct=EBI-6509505, EBI-739546;
CC Q0VD86; P17931: LGALS3; NbExp=3; IntAct=EBI-6509505, EBI-1170392;
CC Q0VD86; Q9UBR4-2: LHX3; NbExp=5; IntAct=EBI-6509505, EBI-12039345;
CC Q0VD86; Q8IXW0: LMNTD2; NbExp=3; IntAct=EBI-6509505, EBI-12028858;
CC Q0VD86; P25791-3: LMO2; NbExp=3; IntAct=EBI-6509505, EBI-11959475;
CC Q0VD86; Q8TAP4-4: LMO3; NbExp=5; IntAct=EBI-6509505, EBI-11742507;
CC Q0VD86; P62310: LSM3; NbExp=3; IntAct=EBI-6509505, EBI-348239;
CC Q0VD86; O95372: LYPLA2; NbExp=3; IntAct=EBI-6509505, EBI-715999;
CC Q0VD86; Q9UI95: MAD2L2; NbExp=3; IntAct=EBI-6509505, EBI-77889;
CC Q0VD86; Q96JG8: MAGED4; NbExp=3; IntAct=EBI-6509505, EBI-721864;
CC Q0VD86; Q96A72: MAGOHB; NbExp=3; IntAct=EBI-6509505, EBI-746778;
CC Q0VD86; Q96EH3: MALSU1; NbExp=3; IntAct=EBI-6509505, EBI-2339737;
CC Q0VD86; Q9Y4F3: MARF1; NbExp=3; IntAct=EBI-6509505, EBI-5235902;
CC Q0VD86; Q9P0L2: MARK1; NbExp=3; IntAct=EBI-6509505, EBI-968587;
CC Q0VD86; O15232: MATN3; NbExp=3; IntAct=EBI-6509505, EBI-6262458;
CC Q0VD86; Q9BUN1: MENT; NbExp=3; IntAct=EBI-6509505, EBI-12022316;
CC Q0VD86; P53582: METAP1; NbExp=3; IntAct=EBI-6509505, EBI-1051435;
CC Q0VD86; Q9BU76: MMTAG2; NbExp=3; IntAct=EBI-6509505, EBI-742459;
CC Q0VD86; Q00013: MPP1; NbExp=3; IntAct=EBI-6509505, EBI-711788;
CC Q0VD86; Q9NZV6: MSRB1; NbExp=3; IntAct=EBI-6509505, EBI-12330065;
CC Q0VD86; Q96QG7: MTMR9; NbExp=3; IntAct=EBI-6509505, EBI-744593;
CC Q0VD86; Q8N6N6: NATD1; NbExp=3; IntAct=EBI-6509505, EBI-8656665;
CC Q0VD86; O43639: NCK2; NbExp=3; IntAct=EBI-6509505, EBI-713635;
CC Q0VD86; Q9BU61: NDUFAF3; NbExp=3; IntAct=EBI-6509505, EBI-2114801;
CC Q0VD86; Q14511-2: NEDD9; NbExp=3; IntAct=EBI-6509505, EBI-11746523;
CC Q0VD86; Q969S2: NEIL2; NbExp=3; IntAct=EBI-6509505, EBI-10281234;
CC Q0VD86; Q9HC98: NEK6; NbExp=4; IntAct=EBI-6509505, EBI-740364;
CC Q0VD86; Q9HC98-4: NEK6; NbExp=3; IntAct=EBI-6509505, EBI-11750983;
CC Q0VD86; Q8NI38: NFKBID; NbExp=3; IntAct=EBI-6509505, EBI-10271199;
CC Q0VD86; Q5HYW2: NHSL2; NbExp=3; IntAct=EBI-6509505, EBI-2859639;
CC Q0VD86; Q9Y5B8: NME7; NbExp=5; IntAct=EBI-6509505, EBI-744782;
CC Q0VD86; Q9NWW6: NMRK1; NbExp=3; IntAct=EBI-6509505, EBI-10315485;
CC Q0VD86; Q99743: NPAS2; NbExp=3; IntAct=EBI-6509505, EBI-3932727;
CC Q0VD86; Q96HA8: NTAQ1; NbExp=5; IntAct=EBI-6509505, EBI-741158;
CC Q0VD86; Q9Y5Y2: NUBP2; NbExp=3; IntAct=EBI-6509505, EBI-1048886;
CC Q0VD86; Q9BRJ7: NUDT16L1; NbExp=3; IntAct=EBI-6509505, EBI-2949792;
CC Q0VD86; Q9BRQ3: NUDT22; NbExp=3; IntAct=EBI-6509505, EBI-10297093;
CC Q0VD86; O95007: OR6B1; NbExp=3; IntAct=EBI-6509505, EBI-12176191;
CC Q0VD86; Q9UJX0: OSGIN1; NbExp=6; IntAct=EBI-6509505, EBI-9057006;
CC Q0VD86; Q9ULW8: PADI3; NbExp=3; IntAct=EBI-6509505, EBI-10488185;
CC Q0VD86; Q8WXA2: PATE1; NbExp=3; IntAct=EBI-6509505, EBI-10277790;
CC Q0VD86; Q92824-2: PCSK5; NbExp=3; IntAct=EBI-6509505, EBI-11956269;
CC Q0VD86; Q99447: PCYT2; NbExp=3; IntAct=EBI-6509505, EBI-750317;
CC Q0VD86; Q9P0J1: PDP1; NbExp=3; IntAct=EBI-6509505, EBI-2861634;
CC Q0VD86; P16284: PECAM1; NbExp=3; IntAct=EBI-6509505, EBI-716404;
CC Q0VD86; Q9UBV8: PEF1; NbExp=3; IntAct=EBI-6509505, EBI-724639;
CC Q0VD86; Q99471: PFDN5; NbExp=3; IntAct=EBI-6509505, EBI-357275;
CC Q0VD86; O43189: PHF1; NbExp=3; IntAct=EBI-6509505, EBI-530034;
CC Q0VD86; O15496: PLA2G10; NbExp=3; IntAct=EBI-6509505, EBI-726466;
CC Q0VD86; Q13393: PLD1; NbExp=3; IntAct=EBI-6509505, EBI-2827556;
CC Q0VD86; Q9NRY6: PLSCR3; NbExp=3; IntAct=EBI-6509505, EBI-750734;
CC Q0VD86; O15160: POLR1C; NbExp=3; IntAct=EBI-6509505, EBI-1055079;
CC Q0VD86; Q9BUI4: POLR3C; NbExp=3; IntAct=EBI-6509505, EBI-5452779;
CC Q0VD86; Q969H6: POP5; NbExp=3; IntAct=EBI-6509505, EBI-366525;
CC Q0VD86; Q12837: POU4F2; NbExp=3; IntAct=EBI-6509505, EBI-17236143;
CC Q0VD86; Q7Z5V6-2: PPP1R32; NbExp=3; IntAct=EBI-6509505, EBI-12000762;
CC Q0VD86; P41219: PRPH; NbExp=3; IntAct=EBI-6509505, EBI-752074;
CC Q0VD86; P49721: PSMB2; NbExp=3; IntAct=EBI-6509505, EBI-359335;
CC Q0VD86; P28062-2: PSMB8; NbExp=3; IntAct=EBI-6509505, EBI-372312;
CC Q0VD86; P43115-12: PTGER3; NbExp=3; IntAct=EBI-6509505, EBI-10234038;
CC Q0VD86; Q9Y2K5: R3HDM2; NbExp=3; IntAct=EBI-6509505, EBI-948428;
CC Q0VD86; P20339: RAB5A; NbExp=3; IntAct=EBI-6509505, EBI-399437;
CC Q0VD86; P51148: RAB5C; NbExp=2; IntAct=EBI-6509505, EBI-1054923;
CC Q0VD86; P63244: RACK1; NbExp=2; IntAct=EBI-6509505, EBI-296739;
CC Q0VD86; P11234: RALB; NbExp=3; IntAct=EBI-6509505, EBI-752162;
CC Q0VD86; Q9BTL3: RAMAC; NbExp=6; IntAct=EBI-6509505, EBI-744023;
CC Q0VD86; Q93062: RBPMS; NbExp=3; IntAct=EBI-6509505, EBI-740322;
CC Q0VD86; Q93062-3: RBPMS; NbExp=3; IntAct=EBI-6509505, EBI-740343;
CC Q0VD86; Q6P9E2: RECK; NbExp=3; IntAct=EBI-6509505, EBI-10253121;
CC Q0VD86; P40938: RFC3; NbExp=3; IntAct=EBI-6509505, EBI-1055010;
CC Q0VD86; P57055: RIPPLY3; NbExp=3; IntAct=EBI-6509505, EBI-12092053;
CC Q0VD86; Q9BY78: RNF26; NbExp=2; IntAct=EBI-6509505, EBI-2129375;
CC Q0VD86; O00422: SAP18; NbExp=3; IntAct=EBI-6509505, EBI-1044156;
CC Q0VD86; Q9BWG6: SCNM1; NbExp=4; IntAct=EBI-6509505, EBI-748391;
CC Q0VD86; Q9UDX3: SEC14L4; NbExp=3; IntAct=EBI-6509505, EBI-10320311;
CC Q0VD86; Q9NUL5-4: SHFL; NbExp=3; IntAct=EBI-6509505, EBI-11955083;
CC Q0VD86; Q8N1D0-2: SLC22A18AS; NbExp=3; IntAct=EBI-6509505, EBI-12829638;
CC Q0VD86; Q9H0W8: SMG9; NbExp=3; IntAct=EBI-6509505, EBI-2872322;
CC Q0VD86; Q53HV7-2: SMUG1; NbExp=3; IntAct=EBI-6509505, EBI-12275818;
CC Q0VD86; P14678-2: SNRPB; NbExp=3; IntAct=EBI-6509505, EBI-372475;
CC Q0VD86; Q99932-2: SPAG8; NbExp=3; IntAct=EBI-6509505, EBI-11959123;
CC Q0VD86; Q9NZD8: SPG21; NbExp=6; IntAct=EBI-6509505, EBI-742688;
CC Q0VD86; Q5T280: SPOUT1; NbExp=2; IntAct=EBI-6509505, EBI-2880213;
CC Q0VD86; O43609: SPRY1; NbExp=3; IntAct=EBI-6509505, EBI-3866665;
CC Q0VD86; Q96FJ0: STAMBPL1; NbExp=3; IntAct=EBI-6509505, EBI-745021;
CC Q0VD86; O75716: STK16; NbExp=6; IntAct=EBI-6509505, EBI-749295;
CC Q0VD86; Q8TCJ2: STT3B; NbExp=3; IntAct=EBI-6509505, EBI-2256290;
CC Q0VD86; P51687: SUOX; NbExp=5; IntAct=EBI-6509505, EBI-3921347;
CC Q0VD86; Q9P0N9: TBC1D7; NbExp=3; IntAct=EBI-6509505, EBI-3258000;
CC Q0VD86; P56279: TCL1A; NbExp=3; IntAct=EBI-6509505, EBI-749995;
CC Q0VD86; Q15561: TEAD4; NbExp=3; IntAct=EBI-6509505, EBI-747736;
CC Q0VD86; Q8WW24: TEKT4; NbExp=3; IntAct=EBI-6509505, EBI-750487;
CC Q0VD86; Q9BXU0: TEX12; NbExp=3; IntAct=EBI-6509505, EBI-12090309;
CC Q0VD86; P05549-5: TFAP2A; NbExp=3; IntAct=EBI-6509505, EBI-12194905;
CC Q0VD86; Q7Z6R9: TFAP2D; NbExp=5; IntAct=EBI-6509505, EBI-11952651;
CC Q0VD86; Q01664: TFAP4; NbExp=3; IntAct=EBI-6509505, EBI-2514218;
CC Q0VD86; Q92734: TFG; NbExp=3; IntAct=EBI-6509505, EBI-357061;
CC Q0VD86; Q8TBB0: THAP6; NbExp=3; IntAct=EBI-6509505, EBI-3925505;
CC Q0VD86; Q92748: THRSP; NbExp=3; IntAct=EBI-6509505, EBI-1749955;
CC Q0VD86; Q01085-2: TIAL1; NbExp=3; IntAct=EBI-6509505, EBI-11064654;
CC Q0VD86; Q8N7U7-2: TPRX1; NbExp=3; IntAct=EBI-6509505, EBI-14115717;
CC Q0VD86; P0DI81-3: TRAPPC2; NbExp=3; IntAct=EBI-6509505, EBI-11961968;
CC Q0VD86; O75865-2: TRAPPC6A; NbExp=3; IntAct=EBI-6509505, EBI-8451480;
CC Q0VD86; Q96RU7: TRIB3; NbExp=3; IntAct=EBI-6509505, EBI-492476;
CC Q0VD86; Q12899: TRIM26; NbExp=2; IntAct=EBI-6509505, EBI-2341136;
CC Q0VD86; Q6AZZ1: TRIM68; NbExp=3; IntAct=EBI-6509505, EBI-2130449;
CC Q0VD86; Q15645: TRIP13; NbExp=3; IntAct=EBI-6509505, EBI-358993;
CC Q0VD86; Q15654: TRIP6; NbExp=3; IntAct=EBI-6509505, EBI-742327;
CC Q0VD86; Q8WW01: TSEN15; NbExp=3; IntAct=EBI-6509505, EBI-372432;
CC Q0VD86; Q99816: TSG101; NbExp=3; IntAct=EBI-6509505, EBI-346882;
CC Q0VD86; Q96PN8: TSSK3; NbExp=3; IntAct=EBI-6509505, EBI-3918381;
CC Q0VD86; Q5T7W7: TSTD2; NbExp=3; IntAct=EBI-6509505, EBI-8994397;
CC Q0VD86; Q9BRA2: TXNDC17; NbExp=3; IntAct=EBI-6509505, EBI-1055906;
CC Q0VD86; Q8TF42: UBASH3B; NbExp=3; IntAct=EBI-6509505, EBI-1380492;
CC Q0VD86; Q9Y2X8: UBE2D4; NbExp=3; IntAct=EBI-6509505, EBI-745527;
CC Q0VD86; Q9H832: UBE2Z; NbExp=3; IntAct=EBI-6509505, EBI-720977;
CC Q0VD86; Q8WUN7: UBTD2; NbExp=3; IntAct=EBI-6509505, EBI-12867288;
CC Q0VD86; Q14CS0: UBXN2B; NbExp=6; IntAct=EBI-6509505, EBI-1993619;
CC Q0VD86; P61960: UFM1; NbExp=3; IntAct=EBI-6509505, EBI-1045061;
CC Q0VD86; Q6NVU6: UFSP1; NbExp=3; IntAct=EBI-6509505, EBI-12068150;
CC Q0VD86; Q9Y4E8: USP15; NbExp=2; IntAct=EBI-6509505, EBI-1043104;
CC Q0VD86; Q14119: VEZF1; NbExp=3; IntAct=EBI-6509505, EBI-11980193;
CC Q0VD86; A8MV65-2: VGLL3; NbExp=3; IntAct=EBI-6509505, EBI-11957216;
CC Q0VD86; P08670: VIM; NbExp=3; IntAct=EBI-6509505, EBI-353844;
CC Q0VD86; O14972: VPS26C; NbExp=3; IntAct=EBI-6509505, EBI-7207091;
CC Q0VD86; Q548N1: VPS28; NbExp=3; IntAct=EBI-6509505, EBI-10243107;
CC Q0VD86; Q9NZC7-5: WWOX; NbExp=3; IntAct=EBI-6509505, EBI-12040603;
CC Q0VD86; Q9GZM5: YIPF3; NbExp=3; IntAct=EBI-6509505, EBI-743787;
CC Q0VD86; Q5VVQ6: YOD1; NbExp=3; IntAct=EBI-6509505, EBI-2510804;
CC Q0VD86; Q6UX98: ZDHHC24; NbExp=3; IntAct=EBI-6509505, EBI-10254561;
CC Q0VD86; Q9H8U3: ZFAND3; NbExp=3; IntAct=EBI-6509505, EBI-725171;
CC Q0VD86; Q9H0C1: ZMYND12; NbExp=3; IntAct=EBI-6509505, EBI-12030590;
CC Q0VD86; Q96E35: ZMYND19; NbExp=3; IntAct=EBI-6509505, EBI-746595;
CC Q0VD86; P17020: ZNF16; NbExp=2; IntAct=EBI-6509505, EBI-3921553;
CC Q0VD86; P17041: ZNF32; NbExp=3; IntAct=EBI-6509505, EBI-1965483;
CC Q0VD86; Q9NWS9-2: ZNF446; NbExp=3; IntAct=EBI-6509505, EBI-740232;
CC Q0VD86; Q9BV97: ZNF747; NbExp=3; IntAct=EBI-6509505, EBI-4395497;
CC Q0VD86; Q7L2R6-2: ZNF765; NbExp=3; IntAct=EBI-6509505, EBI-12834294;
CC Q0VD86; O60232: ZNRD2; NbExp=3; IntAct=EBI-6509505, EBI-741415;
CC Q0VD86; G4XUV3; NbExp=3; IntAct=EBI-6509505, EBI-10177989;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15159402,
CC ECO:0000269|PubMed:18756329}. Cytoplasm {ECO:0000269|PubMed:18756329}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0VD86-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0VD86-2; Sequence=VSP_033235;
CC -!- TISSUE SPECIFICITY: Detected in testis, and at lower levels in ovary.
CC Detected at very low levels in testis tumors (PubMed:15159402). Down-
CC regulated in bone marrow cells in acute myeloid and lymphoid leukemia
CC patients as compared with normal bone marrow cells (PubMed:21540187).
CC {ECO:0000269|PubMed:15159402, ECO:0000269|PubMed:21540187}.
CC -!- INDUCTION: By serum starvation. {ECO:0000269|PubMed:21540187}.
CC -!- PTM: Phosphorylated when part of a complex with CCNA1 and CDK2.
CC Strongly phosphorylated by CDK2 on its C-terminal region spanning amino
CC acid 149-221. Less intensively phosphorylated by CDK2 on its first 75
CC amino acid residues. {ECO:0000269|PubMed:15159402}.
CC -!- SIMILARITY: Belongs to the INCA family. {ECO:0000305}.
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DR EMBL; AY601906; AAT09152.1; -; mRNA.
DR EMBL; AY601907; AAT09153.1; -; mRNA.
DR EMBL; AY604176; AAT36739.1; -; mRNA.
DR EMBL; BC119781; AAI19782.1; -; mRNA.
DR EMBL; BC122873; AAI22874.1; -; mRNA.
DR CCDS; CCDS11064.1; -. [Q0VD86-2]
DR CCDS; CCDS54074.1; -. [Q0VD86-1]
DR RefSeq; NP_001161457.1; NM_001167985.1. [Q0VD86-2]
DR RefSeq; NP_001161458.1; NM_001167986.1. [Q0VD86-1]
DR RefSeq; NP_001161459.1; NM_001167987.1. [Q0VD86-1]
DR RefSeq; NP_998891.2; NM_213726.2. [Q0VD86-2]
DR RefSeq; XP_005256684.1; XM_005256627.3. [Q0VD86-1]
DR RefSeq; XP_005256685.1; XM_005256628.4. [Q0VD86-1]
DR RefSeq; XP_005256686.1; XM_005256629.1. [Q0VD86-2]
DR RefSeq; XP_006721580.1; XM_006721517.1. [Q0VD86-1]
DR RefSeq; XP_006721581.1; XM_006721518.2. [Q0VD86-2]
DR RefSeq; XP_006721582.1; XM_006721519.3. [Q0VD86-2]
DR RefSeq; XP_011522134.1; XM_011523832.2. [Q0VD86-1]
DR AlphaFoldDB; Q0VD86; -.
DR BioGRID; 132642; 239.
DR ELM; Q0VD86; -.
DR IntAct; Q0VD86; 252.
DR MINT; Q0VD86; -.
DR STRING; 9606.ENSP00000458316; -.
DR iPTMnet; Q0VD86; -.
DR PhosphoSitePlus; Q0VD86; -.
DR BioMuta; INCA1; -.
DR DMDM; 121940471; -.
DR PaxDb; Q0VD86; -.
DR PeptideAtlas; Q0VD86; -.
DR PRIDE; Q0VD86; -.
DR Antibodypedia; 51738; 136 antibodies from 20 providers.
DR DNASU; 388324; -.
DR Ensembl; ENST00000355025.7; ENSP00000347129.3; ENSG00000196388.8. [Q0VD86-2]
DR Ensembl; ENST00000574617.1; ENSP00000458316.1; ENSG00000196388.8. [Q0VD86-1]
DR Ensembl; ENST00000575780.5; ENSP00000461844.1; ENSG00000196388.8. [Q0VD86-2]
DR Ensembl; ENST00000576820.5; ENSP00000460673.1; ENSG00000196388.8. [Q0VD86-1]
DR GeneID; 388324; -.
DR KEGG; hsa:388324; -.
DR UCSC; uc002gal.3; human. [Q0VD86-1]
DR CTD; 388324; -.
DR DisGeNET; 388324; -.
DR GeneCards; INCA1; -.
DR HGNC; HGNC:32224; INCA1.
DR HPA; ENSG00000196388; Tissue enriched (testis).
DR MalaCards; INCA1; -.
DR MIM; 617374; gene.
DR neXtProt; NX_Q0VD86; -.
DR OpenTargets; ENSG00000196388; -.
DR PharmGKB; PA165431930; -.
DR VEuPathDB; HostDB:ENSG00000196388; -.
DR eggNOG; ENOG502SX61; Eukaryota.
DR GeneTree; ENSGT00390000000242; -.
DR HOGENOM; CLU_103802_0_0_1; -.
DR InParanoid; Q0VD86; -.
DR OMA; TWMEEQY; -.
DR OrthoDB; 1275701at2759; -.
DR PhylomeDB; Q0VD86; -.
DR TreeFam; TF343431; -.
DR PathwayCommons; Q0VD86; -.
DR SignaLink; Q0VD86; -.
DR BioGRID-ORCS; 388324; 13 hits in 1073 CRISPR screens.
DR ChiTaRS; INCA1; human.
DR GenomeRNAi; 388324; -.
DR Pharos; Q0VD86; Tbio.
DR PRO; PR:Q0VD86; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q0VD86; protein.
DR Bgee; ENSG00000196388; Expressed in right testis and 96 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016604; C:nuclear body; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030332; F:cyclin binding; IDA:MGI.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0044877; F:protein-containing complex binding; IPI:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:MGI.
DR InterPro; IPR026238; INCA1.
DR PANTHER; PTHR37341; PTHR37341; 1.
DR Pfam; PF15142; INCA1; 1.
DR PRINTS; PR02102; PROTEININCA1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Phosphoprotein;
KW Protein kinase inhibitor; Reference proteome.
FT CHAIN 1..236
FT /note="Protein INCA1"
FT /id="PRO_0000331513"
FT REGION 75..99
FT /note="Interaction with CCNA1 and CCNA1/CDK2 complex;
FT essential for CDK2 inhibitory activity"
FT /evidence="ECO:0000269|PubMed:21540187"
FT MOTIF 90..95
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:15159402"
FT MOD_RES 182
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:15159402"
FT MOD_RES 191
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:15159402"
FT MOD_RES 194
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:15159402"
FT VAR_SEQ 67..81
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15159402, ECO:0000303|Ref.2"
FT /id="VSP_033235"
FT MUTAGEN 23
FT /note="S->A: Loss of phosphorylation site."
FT /evidence="ECO:0000269|PubMed:15159402"
FT MUTAGEN 182
FT /note="T->A: Reduced phosphorylation. Phosphorylation is
FT almost abolished; when associated with A-191."
FT /evidence="ECO:0000269|PubMed:15159402"
FT MUTAGEN 191
FT /note="S->A: Strongly reduced phosphorylation.
FT Phosphorylation is almost abolished; when associated with
FT A-182."
FT /evidence="ECO:0000269|PubMed:15159402"
FT MUTAGEN 194
FT /note="S->A: Reduced phosphorylation."
FT /evidence="ECO:0000269|PubMed:15159402"
FT CONFLICT 153
FT /note="E -> D (in Ref. 1; AAT09152/AAT09153)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 236 AA; 26817 MW; A9808D24144AB07D CRC64;
MQVQDDGVNL IPFAKCSRVV SRSPPPRLPS QSLRPMPQRY GDVFWKNLNQ RPTPTWLEEQ
HIPPMLRATG CSQLGLYPPE QLPPPEMLWR RKKRRPCLEG MQQQGLGGVP ARVRAVTYHL
EDLRRRQSII NELKKAQWGS SGAASEPVVL GEEGCGFPST NEYPDLEEER ATYPQEEDRF
LTPGRAQLLW SPWSPLDQEE ACASRQLHSL ASFSTVTARR NPLHNPWGME LAASEE
