ID   INCA_CHLTH              Reviewed;         273 AA.
AC   P0DPS5; O69196; Q9AMA4; Q9AMA5; Q9AMA6; Q9AMB2;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Inclusion membrane protein A;
GN   Name=incA;
OS   Chlamydia trachomatis.
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=B/Tw-5/OT, L1/440/LN, and L3/404;
RX   PubMed=11402010; DOI=10.1128/iai.69.7.4654-4656.2001;
RA   Pannekoek Y., van der Ende A., Eijk P.P., van Marle J., de Witte M.A.,
RA   Ossewaarde J.M., van den Brule A.J.C., Morre S.A., Dankert J.;
RT   "Normal IncA expression and fusogenicity of inclusions in Chlamydia
RT   trachomatis isolates with the incA I47T mutation.";
RL   Infect. Immun. 69:4654-4656(2001).
RN   [2]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=A/G-17/OT, B/Tw-5/OT, Ba/Ap-2/OT, C/TW-3/OT, D-/MT 157/Cx,
RC   Da/TW-448/Cx, E/UW-5/Cx, F/UW-6/Cx, G/UW-57/Cx, H/UW-4/Cx, I-/MT 518/Cx,
RC   I/UW-12/Ur, Ia/UW-202/NP, J/UW-36/Cx, K/UW-31/Cx, L1/440/Bu, L2a/UW-396/Bu,
RC   and L3/404/Bu;
RX   PubMed=9826388; DOI=10.1128/iai.66.12.6017-6021.1998;
RA   Bannantine J.P., Stamm W.E., Suchland R.J., Rockey D.D.;
RT   "Chlamydia trachomatis IncA is localized to the inclusion membrane and is
RT   recognized by antisera from infected humans and primates.";
RL   Infect. Immun. 66:6017-6021(1998).
CC   -!- FUNCTION: Chlamydia replicate within a host intracellular vacuole,
CC       termed an inclusion. IncA is probably involved in the homotypic fusion
CC       of inclusions. {ECO:0000250|UniProtKB:A0A0H3MD02}.
CC   -!- SUBUNIT: Forms homooligomers. {ECO:0000250|UniProtKB:A0A0H3MD02}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:A0A0H3MD02}. Host
CC       vacuole, host pathogen-containing vacuole, host pathogen-containing
CC       vacuole membrane {ECO:0000269|PubMed:9826388,
CC       ECO:0000305|PubMed:11402010}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Secreted, probably by a type III secretion system.
CC       Localized in the inclusion membrane (Probable) (PubMed:9826388). In the
CC       inclusion, the C-terminus faces the host cytosol (By similarity).
CC       {ECO:0000250|UniProtKB:A0A0H3MD02, ECO:0000269|PubMed:9826388,
CC       ECO:0000305|PubMed:11402010}.
CC   -!- DOMAIN: IncA proteins share the same general organization: a short N-
CC       terminal domain, a large bilobed hydrophobic domain, and a C-terminal
CC       cytoplasmic domain. {ECO:0000250|UniProtKB:A0A0H3MD02}.
CC   -!- SIMILARITY: Belongs to the IncA family. {ECO:0000305}.
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DR   EMBL; AF327008; AAG61105.1; -; Genomic_DNA.
DR   EMBL; AF327010; AAG61107.1; -; Genomic_DNA.
DR   EMBL; AF326993; AAG61090.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0DPS5; -.
DR   SMR; P0DPS5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0140221; C:pathogen-containing vacuole membrane; IDA:UniProtKB.
PE   3: Inferred from homology;
KW   Coiled coil; Host membrane; Membrane; Secreted; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..273
FT                   /note="Inclusion membrane protein A"
FT                   /id="PRO_0000084197"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          254..273
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          170..233
FT                   /evidence="ECO:0000255"
FT   VARIANT         200
FT                   /note="R -> C (in strain: L1/440-L)"
FT   VARIANT         235
FT                   /note="R -> K (in strain: L1/440/LN and B/Tw-5/OT)"
SQ   SEQUENCE   273 AA;  30346 MW;  7E9F9569405E5469 CRC64;
     MTTPTLIVTP PSPPAPSYSA NRVPQPSLMD KIKKIAAIAS LILIGTIGFL ALLGHLVGFL
     IAPQITIVLL ALFITSLAGN ALYLQKTANL HLYQDLQREV GSLKEINFML SVLQKEFLHL
     SKEFATTSKD LSAVSQDFYS CLQGFRDNYK GFESLLDEYK NSTEEMRKLF SQEIIADLKS
     SVASLREEIR FLTPLAEEVR RLAHNRESLT AAIEELKTIR DSLRDEIGQL SQLSRTLTSQ
     IALQRKESSD LCSQIRETLS SPRKSASPST KSS