INCA_CHLTR
ID INCA_CHLTR Reviewed; 273 AA.
AC P0CI27; O84121; Q99Q56; Q9AM94; Q9AMA7; Q9AMA8; Q9AMA9; Q9AMB0; Q9AMB1;
AC Q9F7K9; Q9RFX7;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Inclusion membrane protein A;
GN Name=incA; OrderedLocusNames=CT_119;
OS Chlamydia trachomatis (strain D/UW-3/Cx).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=272561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=D(s)/2923;
RX PubMed=10603409; DOI=10.1128/iai.68.1.360-367.2000;
RA Suchland R.J., Rockey D.D., Bannantine J.P., Stamm W.E.;
RT "Isolates of Chlamydia trachomatis that occupy nonfusogenic inclusions lack
RT IncA, a protein localized to the inclusion membrane.";
RL Infect. Immun. 68:360-367(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=A/Sa-1, Ba/Apache-2, C/UW-1, D', D-/NL-326, D/IC-CAL8, Da/MT-566,
RC E/10a, E/11a, E/12a, E/2b, E/4a, E/7a, E/DK-20, F/MRC-301, G/IOL-238,
RC H/UW-4, I', I/UW-12, J/UW-36, and K/UW-31;
RX PubMed=11402010; DOI=10.1128/iai.69.7.4654-4656.2001;
RA Pannekoek Y., van der Ende A., Eijk P.P., van Marle J., de Witte M.A.,
RA Ossewaarde J.M., van den Brule A.J.C., Morre S.A., Dankert J.;
RT "Normal IncA expression and fusogenicity of inclusions in Chlamydia
RT trachomatis isolates with the incA I47T mutation.";
RL Infect. Immun. 69:4654-4656(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B9227, D-9291, F9334, G(s)/459, Ia/9309, J(s)/5942, J9329, J9336,
RC and J9346;
RX PubMed=12177343; DOI=10.1099/00221287-148-8-2497;
RA Rockey D.D., Viratyosin W., Bannantine J.P., Suchland R.J., Stamm W.E.;
RT "Diversity within inc genes of clinical Chlamydia trachomatis variant
RT isolates that occupy non-fusogenic inclusions.";
RL Microbiology 148:2497-2505(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D/UW-3/Cx;
RX PubMed=9784136; DOI=10.1126/science.282.5389.754;
RA Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L.,
RA Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.;
RT "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia
RT trachomatis.";
RL Science 282:754-759(1998).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=D/UW-3/Cx;
RX PubMed=9826388; DOI=10.1128/iai.66.12.6017-6021.1998;
RA Bannantine J.P., Stamm W.E., Suchland R.J., Rockey D.D.;
RT "Chlamydia trachomatis IncA is localized to the inclusion membrane and is
RT recognized by antisera from infected humans and primates.";
RL Infect. Immun. 66:6017-6021(1998).
CC -!- FUNCTION: Chlamydia replicate within an intracellular vacuole, termed
CC an inclusion. IncA is probably involved in the homotypic fusion of
CC inclusions. {ECO:0000250|UniProtKB:A0A0H3MD02}.
CC -!- SUBUNIT: Forms homooligomers. {ECO:0000250|UniProtKB:A0A0H3MD02}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:A0A0H3MD02}. Host
CC vacuole, host pathogen-containing vacuole, host pathogen-containing
CC vacuole membrane {ECO:0000269|PubMed:9826388,
CC ECO:0000305|PubMed:10603409, ECO:0000305|PubMed:11402010}; Multi-pass
CC membrane protein {ECO:0000255}. Note=Secreted, probably by a type III
CC secretion system (Probable) (PubMed:9826388). Localized in the
CC inclusion membrane (Probable) (PubMed:9826388). In the inclusion, the
CC C-terminus faces the host cytosol (By similarity).
CC {ECO:0000250|UniProtKB:A0A0H3MD02, ECO:0000269|PubMed:9826388,
CC ECO:0000305|PubMed:10603409, ECO:0000305|PubMed:11402010}.
CC -!- DOMAIN: IncA proteins share the same general organization: a short N-
CC terminal domain, a large bilobed hydrophobic domain, and a C-terminal
CC cytoplasmic domain. {ECO:0000250|UniProtKB:A0A0H3MD02}.
CC -!- SIMILARITY: Belongs to the IncA family. {ECO:0000305}.
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DR EMBL; AF163773; AAD48440.1; -; Genomic_DNA.
DR EMBL; AF326992; AAG61089.1; -; Genomic_DNA.
DR EMBL; AF326994; AAG61091.1; -; Genomic_DNA.
DR EMBL; AF326995; AAG61092.1; -; Genomic_DNA.
DR EMBL; AF326996; AAG61093.1; -; Genomic_DNA.
DR EMBL; AF326997; AAG61094.1; -; Genomic_DNA.
DR EMBL; AF326998; AAG61095.1; -; Genomic_DNA.
DR EMBL; AF326999; AAG61096.1; -; Genomic_DNA.
DR EMBL; AF327000; AAG61097.1; -; Genomic_DNA.
DR EMBL; AF327001; AAG61098.1; -; Genomic_DNA.
DR EMBL; AF327002; AAG61099.1; -; Genomic_DNA.
DR EMBL; AF327003; AAG61100.1; -; Genomic_DNA.
DR EMBL; AF327004; AAG61101.1; -; Genomic_DNA.
DR EMBL; AF327005; AAG61102.1; -; Genomic_DNA.
DR EMBL; AF327006; AAG61103.1; -; Genomic_DNA.
DR EMBL; AF327007; AAG61104.1; -; Genomic_DNA.
DR EMBL; AF327011; AAG61108.1; -; Genomic_DNA.
DR EMBL; AF327012; AAG61109.1; -; Genomic_DNA.
DR EMBL; AF327013; AAG61110.1; -; Genomic_DNA.
DR EMBL; AF327014; AAG61111.1; -; Genomic_DNA.
DR EMBL; AF327015; AAG61112.1; -; Genomic_DNA.
DR EMBL; AF327016; AAG61113.1; -; Genomic_DNA.
DR EMBL; AF327326; AAK11230.1; -; Genomic_DNA.
DR EMBL; AF327327; AAK11231.1; -; Genomic_DNA.
DR EMBL; AF327328; AAK11232.1; -; Genomic_DNA.
DR EMBL; AF327330; AAK11234.1; -; Genomic_DNA.
DR EMBL; AF327331; AAK11235.1; -; Genomic_DNA.
DR EMBL; AF327332; AAK11236.1; -; Genomic_DNA.
DR EMBL; AF327333; AAK11237.1; -; Genomic_DNA.
DR EMBL; AF279346; AAG31466.1; -; Genomic_DNA.
DR EMBL; AF279354; AAG31469.1; -; Genomic_DNA.
DR EMBL; AE001273; AAC67710.1; -; Genomic_DNA.
DR PIR; C71553; C71553.
DR RefSeq; NP_219622.1; NC_000117.1.
DR RefSeq; WP_009871466.1; NC_000117.1.
DR PDB; 6E6A; X-ray; 1.95 A; A/B=87-246.
DR PDB; 6E7E; X-ray; 1.12 A; A=87-246.
DR PDBsum; 6E6A; -.
DR PDBsum; 6E7E; -.
DR AlphaFoldDB; P0CI27; -.
DR SMR; P0CI27; -.
DR IntAct; P0CI27; 3.
DR MINT; P0CI27; -.
DR STRING; 813.O172_00645; -.
DR EnsemblBacteria; AAC67710; AAC67710; CT_119.
DR GeneID; 884147; -.
DR KEGG; ctr:CT_119; -.
DR PATRIC; fig|272561.5.peg.131; -.
DR HOGENOM; CLU_067042_0_0_0; -.
DR Proteomes; UP000000431; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0140221; C:pathogen-containing vacuole membrane; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Host membrane; Membrane; Reference proteome;
KW Secreted; Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..273
FT /note="Inclusion membrane protein A"
FT /id="PRO_0000084196"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 254..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 172..233
FT /evidence="ECO:0000255"
FT VARIANT 47
FT /note="I -> T (in strain: D(s)/2923, D/IC-CAL8, D', F9334,
FT D-9291, J9336, J9346, J(s)/5942, E/DK-20, F/MRC-301, E/4a,
FT E/12a and E/7a)"
FT VARIANT 62
FT /note="A -> T (in strain: H/UW-4)"
FT VARIANT 90
FT /note="L -> P (in strain: C/UW-1)"
FT VARIANT 116
FT /note="E -> K (in strain: D(s)2923, D/IC-CAL8, D', F9334,
FT D-9291, J9336 and J9346)"
FT VARIANT 119
FT /note="H -> L (in strain: Da/MT-566)"
FT VARIANT 180
FT /note="G -> D (in strain: Da/MT-566)"
FT VARIANT 218
FT /note="T -> I (in strain: J9329 and Ia/9309)"
FT VARIANT 222
FT /note="S -> N (in strain: B9227)"
FT VARIANT 252
FT /note="C -> R (in strain: C/UW-1)"
FT VARIANT 260
FT /note="S -> P (in strain: K/UW-31)"
FT VARIANT 261
FT /note="S -> F (in strain: I/UW-12)"
FT HELIX 89..123
FT /evidence="ECO:0007829|PDB:6E7E"
FT TURN 124..127
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 129..142
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 145..149
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 150..158
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 164..167
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 172..192
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 195..204
FT /evidence="ECO:0007829|PDB:6E7E"
FT HELIX 210..246
FT /evidence="ECO:0007829|PDB:6E7E"
SQ SEQUENCE 273 AA; 30327 MW; 6945AE7E8B8BEBF3 CRC64;
MTTPTLIVTP PSPPAPSYSA NRVPQPSLMD KIKKIAAIAS LILIGTIGFL ALLGHLVGFL
IAPQITIVLL ALFIISLAGN ALYLQKTANL HLYQDLQREV GSLKEINFML SVLQKEFLHL
SKEFATTSKD LSAVSQDFYS CLQGFRDNYK GFESLLDEYK NSTEEMRKLF SQEIIADLKG
SVASLREEIR FLTPLAEEVR RLAHNQQSLT VVIEELKTIR DSLRDEIGQL SQLSKTLTSQ
IALQRKESSD LCSQIRETLS SPRKSASPST KSS
