INDY2_DROME
ID INDY2_DROME Reviewed; 562 AA.
AC Q9VDQ0; A4V353; Q95T83;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein I'm not dead yet 2;
GN Name=Indy-2; ORFNames=CG33933;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Head {ECO:0000269|PubMed:12537569}, and
RC Testis {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=11118146; DOI=10.1126/science.290.5499.2137;
RA Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L.;
RT "Extended life-span conferred by cotransporter gene mutations in
RT Drosophila.";
RL Science 290:2137-2140(2000).
CC -!- FUNCTION: Cation-independent electroneutral transporter (not associated
CC with membrane depolarization) of a variety of tricarboxylic and
CC dicarboxylic acid-cycle intermediates. There is also small, but
CC detectable, transport of monocarboxylics. Transport is through the
CC epithelium of the gut and across the plasma membranes of organs
CC involved in intermediary metabolism and storage (By similarity).
CC {ECO:0000250|UniProtKB:Q9VVT2}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC NADC subfamily. {ECO:0000305}.
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DR EMBL; AE014297; AAN13819.1; -; Genomic_DNA.
DR EMBL; AE014297; AAZ52533.1; -; Genomic_DNA.
DR EMBL; AY060287; AAL25326.1; -; mRNA.
DR EMBL; BT001329; AAN71084.1; -; mRNA.
DR RefSeq; NP_001027201.1; NM_001032030.1.
DR RefSeq; NP_001027202.1; NM_001032031.2.
DR AlphaFoldDB; Q9VDQ0; -.
DR SMR; Q9VDQ0; -.
DR IntAct; Q9VDQ0; 1.
DR STRING; 7227.FBpp0099666; -.
DR PaxDb; Q9VDQ0; -.
DR PRIDE; Q9VDQ0; -.
DR EnsemblMetazoa; FBtr0091937; FBpp0099666; FBgn0260466.
DR EnsemblMetazoa; FBtr0091938; FBpp0099667; FBgn0260466.
DR GeneID; 3772456; -.
DR KEGG; dme:Dmel_CG33933; -.
DR CTD; 3772456; -.
DR FlyBase; FBgn0260466; Indy-2.
DR VEuPathDB; VectorBase:FBgn0260466; -.
DR eggNOG; KOG1281; Eukaryota.
DR GeneTree; ENSGT01030000234550; -.
DR HOGENOM; CLU_005170_9_1_1; -.
DR InParanoid; Q9VDQ0; -.
DR OMA; YCELAFH; -.
DR PhylomeDB; Q9VDQ0; -.
DR Reactome; R-DME-433137; Sodium-coupled sulphate, di- and tri-carboxylate transporters.
DR BioGRID-ORCS; 3772456; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 3772456; -.
DR PRO; PR:Q9VDQ0; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0260466; Expressed in testis and 5 other tissues.
DR Genevisible; Q9VDQ0; DM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0015141; F:succinate transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0098656; P:anion transmembrane transport; IBA:GO_Central.
DR GO; GO:0015746; P:citrate transport; ISS:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0006848; P:pyruvate transport; ISS:UniProtKB.
DR GO; GO:0015744; P:succinate transport; ISS:UniProtKB.
DR InterPro; IPR031312; Na/sul_symport_CS.
DR InterPro; IPR001898; SLC13A/DASS.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR PROSITE; PS01271; NA_SULFATE; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..562
FT /note="Protein I'm not dead yet 2"
FT /id="PRO_0000172500"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 562 AA; 61935 MW; D9F04C05FAA73ED3 CRC64;
MAEPGEQRKF VLGRCCIFHW RGKASIIIPL ITLPILIYGF QTDMAEFKCL WLIVTMALLW
ITETLPIYVT ALFPLVFCPL LGLVNASIVC KQYFTDTIVV FLGGLIVALG IEYSNLHTRI
ALRVIRIVGG SPRRLFVGLM SVSTFMGLWI SNSAGTAMMC PIVKALVNEL DTNKIFPVYM
TQEEEPVEEG EPPHPSKITV AFYAGIAYAS SIGGLGTLIG TGTNLVFRGI YTERFPTSTV
EITFANFMFY SIPLMVIVNV TLVIIAFLIT HMGLFRPNSK TGKIIAEANT NRKLMEDVLR
QRHIDLGPMS CHEIQMAIAF AFMIVLLITR KPGFVPGWSD LINRKVVGSA SGLSFIVLLI
FALPTQYTFF KYCCGKGPFT AQAIDAILSW EYVLRNIPWG LLFLLGGGFA LAVASRETGL
NIMISKAMQV LIGLPNIVVQ SITFVLANFF SAFNANVVVA NIVLPILCEM SLALELHPLI
LTLPACLGIS MVYFLPVSTP PNAIVTQYAH IKTKYFACCG IVPTIIGISV ALVNTNTWGL
IIFPESKSFP DWAKEIKNQT KI
