INE_DROME
ID INE_DROME Reviewed; 943 AA.
AC Q9VR07; A4V056; O02003; Q94917;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Sodium- and chloride-dependent GABA transporter ine;
DE AltName: Full=Protein inebriated;
DE AltName: Full=Protein receptor oscillation A;
GN Name=ine {ECO:0000312|FlyBase:FBgn0011603};
GN Synonyms=rosA {ECO:0000312|EMBL:CAA69649.1}; ORFNames=CG15444;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAC47292.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), PUTATIVE FUNCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=Canton-S {ECO:0000312|EMBL:AAC47292.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:1334137};
RX PubMed=1334137; DOI=10.3109/01677069209083445;
RA Stern M., Ganetzky B.;
RT "Identification and characterization of inebriated, a gene affecting
RT neuronal excitability in Drosophila.";
RL J. Neurogenet. 8:157-172(1992).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAA69649.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), PUTATIVE FUNCTION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Canton-S {ECO:0000312|EMBL:CAA69649.1};
RC TISSUE=Head {ECO:0000312|EMBL:CAA69649.1};
RX PubMed=10876650; DOI=10.3109/01677069609107063;
RA Burg M.G., Geng C., Guan Y., Koliantz G., Pak W.L.;
RT "Drosophila rosA gene, which when mutant causes aberrant photoreceptor
RT oscillation, encodes a novel neurotransmitter transporter homologue.";
RL J. Neurogenet. 11:59-79(1996).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAC47292.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), PUTATIVE FUNCTION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Canton-S {ECO:0000312|EMBL:AAC47292.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:8917579};
RX PubMed=8917579; DOI=10.1073/pnas.93.23.13262;
RA Soehnge H., Huang X., Becker M., Whitley P., Conover D., Stern M.;
RT "A neurotransmitter transporter encoded by the Drosophila inebriated
RT gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:13262-13267(1996).
RN [4] {ECO:0000312|EMBL:AAF51001.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5] {ECO:0000305, ECO:0000312|EMBL:AAF51001.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6] {ECO:0000305, ECO:0000312|EMBL:AAM75025.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM75025.1};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7] {ECO:0000305, ECO:0000312|EMBL:AAQ22424.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAQ22424.1}; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000305}
RP FUNCTION.
RX PubMed=11517334; DOI=10.1073/pnas.191107698;
RA Yager J., Richards S., Hekmat-Scafe D.S., Hurd D.D., Sundaresan V.,
RA Caprette D.R., Saxton W.M., Carlson J.R., Stern M.;
RT "Control of Drosophila perineurial glial growth by interacting
RT neurotransmitter-mediated signaling pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:10445-10450(2001).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11861562; DOI=10.1093/genetics/160.2.561;
RA Huang X., Huang Y., Chinnappan R., Bocchini C., Gustin M.C., Stern M.;
RT "The Drosophila inebriated-encoded neurotransmitter/osmolyte transporter:
RT dual roles in the control of neuronal excitability and the osmotic stress
RT response.";
RL Genetics 160:561-569(2002).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11880499; DOI=10.1523/jneurosci.22-05-01698.2002;
RA Huang Y., Stern M.;
RT "In vivo properties of the Drosophila inebriated-encoded neurotransmitter
RT transporter.";
RL J. Neurosci. 22:1698-1708(2002).
RN [11]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-476, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=17893096; DOI=10.1093/glycob/cwm097;
RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L.,
RA Panin V.;
RT "Identification of N-glycosylated proteins from the central nervous system
RT of Drosophila melanogaster.";
RL Glycobiology 17:1388-1403(2007).
CC -!- FUNCTION: Plays a role in neuronal membrane excitation, important for
CC normal response properties of the photoreceptor. Able to control
CC excitability from either neurons or glia cells. Ine negatively
CC regulates neuronal sodium channels. Controls neurotransmitter-mediated
CC signaling pathways associated with the structure of the larval
CC peripheral nerve, ine and eag control perineurial glial growth through
CC partially redundant pathways. Isoform A and isoform B are both
CC functional, although isoform A functions with greater efficiency. Has a
CC role in osmolyte transport within the Malpighian tubule and hindgut.
CC {ECO:0000269|PubMed:11517334, ECO:0000269|PubMed:11861562,
CC ECO:0000269|PubMed:11880499}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000269|PubMed:10876650}; Synonyms=C, D, P1;
CC IsoId=Q9VR07-1; Sequence=Displayed;
CC Name=B {ECO:0000269|PubMed:1334137, ECO:0000269|PubMed:8917579};
CC Synonyms=P2;
CC IsoId=Q9VR07-2; Sequence=VSP_052087, VSP_052088;
CC -!- TISSUE SPECIFICITY: Expressed both maternally and zygotically.
CC Developing embryos exhibit expression in the posterior hindgut,
CC foregut, midgut, Malpighian tubules, anal plate, Garland cells, and a
CC subset of cells in the central nervous system. Central nervous system
CC expression is seen in segmentally repeating in cells flanking the
CC midline of the ventral ganglion. Isoform A and isoform B are
CC colocalized in both the nervous system and the fluid reabsorption
CC system. {ECO:0000269|PubMed:10876650, ECO:0000269|PubMed:11861562,
CC ECO:0000269|PubMed:11880499, ECO:0000269|PubMed:8917579}.
CC -!- DISRUPTION PHENOTYPE: Flies lacking this protein, in a wild-type
CC background, display no observable behavioral defects. In a Shaker
CC mutant background, flies lacking ine exhibit downturned wings and an
CC indented thorax. Flies lacking ine exhibit increased excitability of
CC the larval peripheral nerve by causing the defective re-uptake of the
CC substrate neurotransmitter of the ine transporter and thus
CC overstimulation by this neurotransmitter. Flies lacking ine increase
CC the growth of the perineurial glial layer of the larval peripheral
CC nerve. Flies overexpressing ine exhibit delayed onset of long-term
CC facilitation and increased failure rate of transmitter release at the
CC larval neuromuscular junction, reduced amplitude of larval nerve
CC compound action potentials, suppression of the leg-shaking behavior of
CC mutants defective in the Shaker-encoded potassium channel, and
CC temperature-sensitive paralysis. {ECO:0000269|PubMed:1334137}.
CC -!- MISCELLANEOUS: [Isoform A]: The long N terminus is uncommon among
CC members of the sodium- and chloride-dependent neurotransmitter
CC transporter family but is required for efficient transporter activity.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. {ECO:0000305}.
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DR EMBL; Y08362; CAA69649.1; -; mRNA.
DR EMBL; U66460; AAC47292.1; -; mRNA.
DR EMBL; AE014134; AAF51001.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10349.1; -; Genomic_DNA.
DR EMBL; AE014134; AAO41159.1; -; Genomic_DNA.
DR EMBL; AE014134; AAO41160.1; -; Genomic_DNA.
DR EMBL; AY128432; AAM75025.1; -; mRNA.
DR EMBL; BT009955; AAQ22424.1; -; mRNA.
DR RefSeq; NP_001162871.1; NM_001169400.2. [Q9VR07-1]
DR RefSeq; NP_477012.1; NM_057664.6. [Q9VR07-1]
DR RefSeq; NP_477013.1; NM_057665.5. [Q9VR07-2]
DR RefSeq; NP_787972.1; NM_175958.3. [Q9VR07-1]
DR RefSeq; NP_787973.1; NM_175959.3. [Q9VR07-1]
DR AlphaFoldDB; Q9VR07; -.
DR SMR; Q9VR07; -.
DR BioGRID; 59853; 4.
DR STRING; 7227.FBpp0077136; -.
DR TCDB; 2.A.22.3.12; the neurotransmitter:sodium symporter (nss) family.
DR GlyGen; Q9VR07; 1 site.
DR iPTMnet; Q9VR07; -.
DR PaxDb; Q9VR07; -.
DR DNASU; 33659; -.
DR EnsemblMetazoa; FBtr0077446; FBpp0077136; FBgn0011603. [Q9VR07-1]
DR EnsemblMetazoa; FBtr0077447; FBpp0077137; FBgn0011603. [Q9VR07-2]
DR EnsemblMetazoa; FBtr0077448; FBpp0077138; FBgn0011603. [Q9VR07-1]
DR EnsemblMetazoa; FBtr0301407; FBpp0290621; FBgn0011603. [Q9VR07-1]
DR EnsemblMetazoa; FBtr0301408; FBpp0290622; FBgn0011603. [Q9VR07-1]
DR GeneID; 33659; -.
DR KEGG; dme:Dmel_CG15444; -.
DR UCSC; CG15444-RA; d. melanogaster.
DR CTD; 33659; -.
DR FlyBase; FBgn0011603; ine.
DR VEuPathDB; VectorBase:FBgn0011603; -.
DR eggNOG; KOG3660; Eukaryota.
DR GeneTree; ENSGT00900000141735; -.
DR HOGENOM; CLU_006855_1_0_1; -.
DR InParanoid; Q9VR07; -.
DR OMA; AFGSMIC; -.
DR PhylomeDB; Q9VR07; -.
DR BioGRID-ORCS; 33659; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33659; -.
DR PRO; PR:Q9VR07; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011603; Expressed in adult hindgut (Drosophila) and 43 other tissues.
DR ExpressionAtlas; Q9VR07; baseline and differential.
DR Genevisible; Q9VR07; DM.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:FlyBase.
DR GO; GO:0005328; F:neurotransmitter:sodium symporter activity; ISS:FlyBase.
DR GO; GO:0005034; F:osmosensor activity; IMP:UniProtKB.
DR GO; GO:0042065; P:glial cell growth; IMP:UniProtKB.
DR GO; GO:0050891; P:multicellular organismal water homeostasis; IMP:FlyBase.
DR GO; GO:0006836; P:neurotransmitter transport; ISM:FlyBase.
DR GO; GO:0042066; P:perineurial glial growth; IGI:FlyBase.
DR GO; GO:0047484; P:regulation of response to osmotic stress; IMP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:FlyBase.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0019226; P:transmission of nerve impulse; IMP:UniProtKB.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002944; Na/ntran_symport_inebriated.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01205; INEBRIATED.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; Glycoprotein;
KW Membrane; Neurogenesis; Neurotransmitter transport; Reference proteome;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..943
FT /note="Sodium- and chloride-dependent GABA transporter ine"
FT /id="PRO_0000245793"
FT TOPO_DOM 1..345
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 439..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 539..559
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 591..607
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 618..638
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 679..699
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 723..743
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 754..774
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 799..819
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 836..856
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 857..943
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 103..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 476
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096"
FT VAR_SEQ 2..28
FT /note="AENKDVSQVLNTTPDQVVINRAPPTGL -> PNRQDYDAQSSKHSEQSFFRF
FT NRVAKV (in isoform B)"
FT /evidence="ECO:0000303|PubMed:1334137,
FT ECO:0000303|PubMed:8917579"
FT /id="VSP_052087"
FT VAR_SEQ 29..313
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:1334137,
FT ECO:0000303|PubMed:8917579"
FT /id="VSP_052088"
FT CONFLICT 3
FT /note="E -> D (in Ref. 1; AAC47292)"
FT /evidence="ECO:0000305"
FT CONFLICT 7
FT /note="V -> A (in Ref. 1; AAC47292)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="V -> I (in Ref. 1; AAC47292)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="H -> Q (in Ref. 1; AAC47292)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 943 AA; 103636 MW; 0CB8AADCC7FA8FCF CRC64;
MAENKDVSQV LNTTPDQVVI NRAPPTGLAP LRHSQLSDSG AESCYEGDEQ ARLIRSSSSR
AHKFIIIPAT PGTPPGSGAV AGPLPFRQTS STTSLAAMAA ALHKQSPLRH TSVRTRPSSE
VLQPGQVLAN QPTAAVSTST VTFTIDDCNE EGDAIPAAAS VPAPVTMPAI SPTPATLTCT
TTTTMAGDSV AVSPLSMELK SRLGSHHSSM RSVVSGYLPG LSDSSGNLVG GVSMATSGLQ
APNPLYMQPQ ASLSGSSYHF HELAGNQIYS DVTSVRSLAS IGIGSTDGRK LVIRRVPTTA
NELFDMVNPQ TPPPLGVDDD DSYLDMSDET AQLKPRQQHW ANKMQFVLAC IGYSVGLGNV
WRFPYMCYKS GGGVFLVPYC IILFICSIPL LFMELSVGQY TGRGPIGALG QLCPLFKGAG
LASVVVSFLM STYYSVIIGY SIYYFFTSFK TEMPWIDCNN RWNTPDCWVP QRKGINASAP
DTSRTPSEEF FENKVLQISG GLEYPGMMRW ELFACLICAW LMVYFATWKS IKSSAKVRYF
TATFPFVLII ILMVRAVTLD GAAEGLRFFF RPKWSELKNA NVWINAASQN FNSLGITFGS
MISFASYNKY NNNILRDTVA VSAVNMITSL LVGIFAFSTL GNLALEQNTN VRDVIGDGPG
MIFVVYPQAM AKMPYAQLWA VMFFFMLLCL GLNSQFAIVE VVVTSIQDGF PRWIKRHLGY
HEIVVLFVCV ISCLFGMPNI IQGGIYYFQL MDHYAASVTI MFLAFCQMIA IAWFYGTGRL
SKNVKQMTGK APSFYLRSCW LVLGPCLLFA IWVLSLINYH EPTYHNGRYT YPDWAYGIGW
MFASFSLICI PGYAVINFLR SSGDTFWERI RNTLRPNIYE CKICGEHHCE HDYPEQEQFM
LAQEMATVYK PTNPHLLNLG QKCGYNAMQA SPSHAEAGGP CGQ
