INK1A_DANRE
ID INK1A_DANRE Reviewed; 308 AA.
AC F1QR98; A1L1Y9; Q1JQ16; Q503M7;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=PAK4-inhibitor inka1 {ECO:0000305};
DE AltName: Full=Induced in neural crest by AP2-alpha protein 1 {ECO:0000303|PubMed:17314132};
DE Short=zinca1 {ECO:0000303|PubMed:17314132};
GN Name=fam212aa {ECO:0000312|ZFIN:ZDB-GENE-060512-186}; Synonyms=inka1a;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17314132; DOI=10.1242/dev.02813;
RA Luo T., Xu Y., Hoffman T.L., Zhang T., Schilling T., Sargent T.D.;
RT "Inca: a novel p21-activated kinase-associated protein required for cranial
RT neural crest development.";
RL Development 134:1279-1289(2007).
CC -!- FUNCTION: Inhibitor of the serine/threonine-protein kinase pak4/pak5
CC (By similarity). Acts by binding pak4/pak5 in a substrate-like manner,
CC inhibiting the protein kinase activity (By similarity). Required for
CC the proper migration of neural crest cells during embryonic
CC development, probably by inhibiting pak4/pak5 (PubMed:17314132).
CC {ECO:0000250|UniProtKB:Q96EL1, ECO:0000305|PubMed:17314132}.
CC -!- SUBUNIT: Interacts with pak4/pak5. {ECO:0000250|UniProtKB:A7LKB2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96EL1}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- INDUCTION: Expression is regulated by the transcription factor AP-2-
CC alpha/tfap2a. {ECO:0000269|PubMed:17314132}.
CC -!- DOMAIN: Contains 2 Inka boxes (also named iBox or inca box). The Inka
CC boxes bind and inhibit PAK4 by binding a substrate-like manner.
CC {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- DISRUPTION PHENOTYPE: Dramatic reduction in craniofacial cartilage
CC formation. {ECO:0000269|PubMed:17314132}.
CC -!- SIMILARITY: Belongs to the INKA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH95263.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CT963136; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC095263; AAH95263.1; ALT_INIT; mRNA.
DR EMBL; BC116520; AAI16521.1; -; mRNA.
DR EMBL; BC129272; AAI29273.1; -; mRNA.
DR RefSeq; NP_001073457.1; NM_001079988.1.
DR AlphaFoldDB; F1QR98; -.
DR STRING; 7955.ENSDARP00000105046; -.
DR PaxDb; F1QR98; -.
DR Ensembl; ENSDART00000124800; ENSDARP00000105046; ENSDARG00000070360.
DR GeneID; 692272; -.
DR KEGG; dre:692272; -.
DR CTD; 692272; -.
DR ZFIN; ZDB-GENE-060512-186; inka1a.
DR eggNOG; ENOG502RXEK; Eukaryota.
DR GeneTree; ENSGT00530000063849; -.
DR HOGENOM; CLU_077157_0_0_1; -.
DR InParanoid; F1QR98; -.
DR OMA; RPRACKL; -.
DR OrthoDB; 1263048at2759; -.
DR TreeFam; TF332839; -.
DR PRO; PR:F1QR98; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 11.
DR Bgee; ENSDARG00000070360; Expressed in olfactory system and 43 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; ISS:UniProtKB.
DR GO; GO:0001502; P:cartilage condensation; IMP:ZFIN.
DR GO; GO:0051216; P:cartilage development; IMP:ZFIN.
DR InterPro; IPR029267; FAM212.
DR InterPro; IPR039201; Inka.
DR PANTHER; PTHR28615; PTHR28615; 1.
DR Pfam; PF15342; FAM212; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..308
FT /note="PAK4-inhibitor inka1"
FT /id="PRO_0000438816"
FT REGION 81..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 164..201
FT /note="Inka box 1"
FT /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT REGION 281..308
FT /note="Inka box 2"
FT /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT CONFLICT 30
FT /note="R -> W (in Ref. 2; AAH95263)"
FT /evidence="ECO:0000305"
FT CONFLICT 141..143
FT /note="TRA -> SRV (in Ref. 2; AAI16521/AAH95263)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="N -> S (in Ref. 2; AAH95263)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="W -> R (in Ref. 2; AAI29273)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="I -> V (in Ref. 2; AAI16521/AAH95263)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="S -> N (in Ref. 2; AAI16521)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="K -> R (in Ref. 2; AAI16521)"
FT /evidence="ECO:0000305"
FT CONFLICT 268
FT /note="P -> S (in Ref. 2; AAI16521)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 308 AA; 34700 MW; 1861277A0AC450EB CRC64;
MLCLQESGDC LRDQMRYMMR SLQDLKHLRR SCVAPPVGPP VRLRACKQLI AQRERRARLR
ISDASEASSY DSACCLSSSL EEEESASDPS AVSSPSSERS LEFDSGYSEA SWQDEGVVLR
RTKNIRVSST ACLRTNQLSN TRARPKSTSD ACLESWTSFE TASDPEDWTT SLLTRGRNRQ
PLVLGDNSFA DLIHNWMDLP ECPEQTELKH SSGRSFAKDF LVNIKRRIAG FSRSADGRRK
SSDVTKLSKS IVPTKRLSCQ IDVQHKMPFF YKSHTGLNEL DTDYYQFSAL MKSGSRTPIV
CNDIIGYI
