INKA1_MOUSE
ID INKA1_MOUSE Reviewed; 282 AA.
AC Q9CX62; Q08EG3; Q80WB2; Q8BPE9;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=PAK4-inhibitor INKA1 {ECO:0000305};
DE AltName: Full=Induced in neural crest by AP2-alpha protein homolog {ECO:0000303|PubMed:17314132};
DE Short=MInca {ECO:0000303|PubMed:17314132};
DE AltName: Full=Inka box actin regulator 1 {ECO:0000312|MGI:MGI:1915426};
GN Name=Inka1 {ECO:0000312|MGI:MGI:1915426}; Synonyms=Fam212a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION, AND INTERACTION WITH PAK4.
RX PubMed=17314132; DOI=10.1242/dev.02813;
RA Luo T., Xu Y., Hoffman T.L., Zhang T., Schilling T., Sargent T.D.;
RT "Inca: a novel p21-activated kinase-associated protein required for cranial
RT neural crest development.";
RL Development 134:1279-1289(2007).
RN [4]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20175189; DOI=10.1002/dvdy.22248;
RA Reid B.S., Sargent T.D., Williams T.;
RT "Generation and characterization of a novel neural crest marker allele,
RT Inka1-LacZ, reveals a role for Inka1 in mouse neural tube closure.";
RL Dev. Dyn. 239:1188-1196(2010).
CC -!- FUNCTION: Inhibitor of the serine/threonine-protein kinase PAK4. Acts
CC by binding PAK4 in a substrate-like manner, inhibiting the protein
CC kinase activity. {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- SUBUNIT: Interacts with PAK4 (PubMed:17314132).
CC {ECO:0000269|PubMed:17314132}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96EL1}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q96EL1}. Note=Mainly nuclear. Relocalizes to the
CC cytoplasm following interaction with PAK4.
CC {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- TISSUE SPECIFICITY: Expressed in tissues of the developing head during
CC neurulation. {ECO:0000269|PubMed:20175189}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the cephalic mesenchyme, heart and
CC paraxial mesoderm prior to 8.5 dpc. Expression is then observed in the
CC migratory neural crest cells and their derivatives. As development
CC progresses, expression is also observed in the limb buds and
CC perichondrial tissue. {ECO:0000269|PubMed:20175189}.
CC -!- DOMAIN: Contains 2 Inka boxes (also named iBox or inca box). The Inka
CC boxes bind and inhibit PAK4 by binding a substrate-like manner.
CC {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype in most cases: most mice are
CC viable and fertile. A low percentage of mice show exencephaly.
CC {ECO:0000269|PubMed:20175189}.
CC -!- MISCELLANEOUS: Expression of Inka1 is not regulated by AP-2-
CC alpha/Tfap2a, as it is the case for orthologous protein in zebrafish
CC and Xenopus. {ECO:0000269|PubMed:20175189}.
CC -!- SIMILARITY: Belongs to the INKA family. {ECO:0000305}.
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DR EMBL; AK020090; BAB31988.1; -; mRNA.
DR EMBL; AK076092; BAC36176.1; -; mRNA.
DR EMBL; BC051538; AAH51538.1; -; mRNA.
DR EMBL; BC115538; AAI15539.1; -; mRNA.
DR CCDS; CCDS52919.1; -.
DR RefSeq; NP_080873.2; NM_026597.3.
DR AlphaFoldDB; Q9CX62; -.
DR STRING; 10090.ENSMUSP00000040433; -.
DR iPTMnet; Q9CX62; -.
DR PhosphoSitePlus; Q9CX62; -.
DR PaxDb; Q9CX62; -.
DR PRIDE; Q9CX62; -.
DR ProteomicsDB; 269486; -.
DR Antibodypedia; 46012; 78 antibodies from 21 providers.
DR Ensembl; ENSMUST00000048568; ENSMUSP00000040433; ENSMUSG00000042106.
DR GeneID; 68176; -.
DR KEGG; mmu:68176; -.
DR UCSC; uc009rnv.2; mouse.
DR CTD; 389119; -.
DR MGI; MGI:1915426; Inka1.
DR VEuPathDB; HostDB:ENSMUSG00000042106; -.
DR eggNOG; ENOG502R5BS; Eukaryota.
DR GeneTree; ENSGT00530000063849; -.
DR HOGENOM; CLU_077157_0_0_1; -.
DR InParanoid; Q9CX62; -.
DR OMA; DVQPRHQ; -.
DR OrthoDB; 1263048at2759; -.
DR PhylomeDB; Q9CX62; -.
DR TreeFam; TF332839; -.
DR BioGRID-ORCS; 68176; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q9CX62; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9CX62; protein.
DR Bgee; ENSMUSG00000042106; Expressed in manus and 199 other tissues.
DR ExpressionAtlas; Q9CX62; baseline and differential.
DR Genevisible; Q9CX62; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; ISO:MGI.
DR GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; ISS:UniProtKB.
DR GO; GO:0021915; P:neural tube development; IMP:MGI.
DR InterPro; IPR029267; FAM212.
DR InterPro; IPR039201; Inka.
DR PANTHER; PTHR28615; PTHR28615; 1.
DR Pfam; PF15342; FAM212; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..282
FT /note="PAK4-inhibitor INKA1"
FT /id="PRO_0000239718"
FT REGION 21..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 163..200
FT /note="Inka box 1"
FT /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT REGION 256..282
FT /note="Inka box 2"
FT /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT COMPBIAS 108..122
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 31..33
FT /note="PLQ -> GTR (in Ref. 2; AAH51538)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="S -> F (in Ref. 1; BAB31988)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 282 AA; 31804 MW; 2852EDCA524FF102 CRC64;
MHSARLDSFL SQLRWELLCA RDTGSPPMSG PLQPKPRTDQ NVQPKRQFRA SDVLEEDSVC
CVEEEEEEGL VAEDKGLPLG CPREHALDWD SGFSEVSGST WREEEPSVPQ RQAPRERPPH
SQRFSVSDIP MRSRAAVTNI PPAHRPRPKS TPDACLEHWQ GLEAEDWTAA LLNRGRSRQP
LVLGDNCFAD LVHNWMELPE ATSEGSDGDV PRARARPPQF LLGLSEQLRR RLARARRTAM
ASKRLSCPPR SEPDLPADIS RFAALMNCRS RQPIIYNDVS YL
