INKA2_HUMAN
ID INKA2_HUMAN Reviewed; 297 AA.
AC Q9NTI7; B3KP38; B4DF94; Q9NTI6;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=PAK4-inhibitor INKA2 {ECO:0000305};
DE AltName: Full=Induced in neural crest by AP2-alpha protein-related homolog {ECO:0000303|PubMed:17314132};
DE Short=HInca-r {ECO:0000303|PubMed:17314132};
DE AltName: Full=Inka box actin regulator 2 {ECO:0000312|HGNC:HGNC:28045};
GN Name=INKA2 {ECO:0000312|HGNC:HGNC:28045};
GN Synonyms=C1orf183 {ECO:0000312|HGNC:HGNC:28045}, FAM212B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Rhodes S., Huckle E.;
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION.
RX PubMed=17314132; DOI=10.1242/dev.02813;
RA Luo T., Xu Y., Hoffman T.L., Zhang T., Schilling T., Sargent T.D.;
RT "Inca: a novel p21-activated kinase-associated protein required for cranial
RT neural crest development.";
RL Development 134:1279-1289(2007).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH PAK4.
RX PubMed=26607847; DOI=10.1038/ncomms9681;
RA Baskaran Y., Ang K.C., Anekal P.V., Chan W.L., Grimes J.M., Manser E.,
RA Robinson R.C.;
RT "An in cellulo-derived structure of PAK4 in complex with its inhibitor
RT Inka1.";
RL Nat. Commun. 6:8681-8681(2015).
CC -!- FUNCTION: Inhibitor of the serine/threonine-protein kinase PAK4. Acts
CC by binding PAK4 in a substrate-like manner, inhibiting the protein
CC kinase activity. {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- SUBUNIT: Interacts with PAK4 (PubMed:26607847).
CC {ECO:0000269|PubMed:26607847}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26607847}.
CC -!- DOMAIN: The Inka box (also named iBox or inca box) binds and inhibits
CC PAK4 by binding a substrate-like manner.
CC {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- SIMILARITY: Belongs to the INKA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAG57355.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB69909.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL137198; CAB69908.1; -; mRNA.
DR EMBL; AL137199; CAB69909.1; ALT_INIT; mRNA.
DR EMBL; AK055667; BAG51550.1; -; mRNA.
DR EMBL; AK293989; BAG57355.1; ALT_INIT; mRNA.
DR EMBL; AL049557; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471122; EAW56505.1; -; Genomic_DNA.
DR EMBL; BC031558; AAH31558.1; -; mRNA.
DR CCDS; CCDS841.1; -.
DR RefSeq; NP_061972.1; NM_019099.4.
DR RefSeq; NP_945120.1; NM_198926.2.
DR AlphaFoldDB; Q9NTI7; -.
DR BioGRID; 121003; 20.
DR IntAct; Q9NTI7; 4.
DR STRING; 9606.ENSP00000349805; -.
DR iPTMnet; Q9NTI7; -.
DR PhosphoSitePlus; Q9NTI7; -.
DR BioMuta; FAM212B; -.
DR MassIVE; Q9NTI7; -.
DR PaxDb; Q9NTI7; -.
DR PeptideAtlas; Q9NTI7; -.
DR PRIDE; Q9NTI7; -.
DR Antibodypedia; 33813; 123 antibodies from 13 providers.
DR DNASU; 55924; -.
DR Ensembl; ENST00000357260.6; ENSP00000349805.5; ENSG00000197852.12.
DR GeneID; 55924; -.
DR KEGG; hsa:55924; -.
DR MANE-Select; ENST00000357260.6; ENSP00000349805.5; NM_019099.5; NP_061972.1.
DR UCSC; uc001ebo.2; human.
DR CTD; 55924; -.
DR DisGeNET; 55924; -.
DR GeneCards; INKA2; -.
DR HGNC; HGNC:28045; INKA2.
DR HPA; ENSG00000197852; Low tissue specificity.
DR neXtProt; NX_Q9NTI7; -.
DR OpenTargets; ENSG00000197852; -.
DR PharmGKB; PA142672431; -.
DR VEuPathDB; HostDB:ENSG00000197852; -.
DR eggNOG; ENOG502RZNT; Eukaryota.
DR GeneTree; ENSGT00530000063849; -.
DR InParanoid; Q9NTI7; -.
DR OMA; EEREICK; -.
DR OrthoDB; 794088at2759; -.
DR PhylomeDB; Q9NTI7; -.
DR TreeFam; TF332839; -.
DR PathwayCommons; Q9NTI7; -.
DR SignaLink; Q9NTI7; -.
DR BioGRID-ORCS; 55924; 24 hits in 1072 CRISPR screens.
DR ChiTaRS; FAM212B; human.
DR GenomeRNAi; 55924; -.
DR Pharos; Q9NTI7; Tdark.
DR PRO; PR:Q9NTI7; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9NTI7; protein.
DR Bgee; ENSG00000197852; Expressed in blood and 140 other tissues.
DR ExpressionAtlas; Q9NTI7; baseline and differential.
DR Genevisible; Q9NTI7; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; ISS:UniProtKB.
DR InterPro; IPR029267; FAM212.
DR InterPro; IPR039201; Inka.
DR PANTHER; PTHR28615; PTHR28615; 1.
DR Pfam; PF15342; FAM212; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..297
FT /note="PAK4-inhibitor INKA2"
FT /id="PRO_0000304994"
FT REGION 82..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 137..180
FT /note="Inka box"
FT /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT REGION 175..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 234..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..108
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..198
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 297 AA; 32759 MW; AC9E28218271E5E2 CRC64;
MTMESREMDC YLRRLKQELM SMKEVGDGLQ DQMNCMMGAL QELKLLQVQT ALEQLEISGG
GPVPGSPEGP RTQCEHPCWE GGRGPARPTV CSPSSQPSLG SSTKFPSHRS VCGRDLAPLP
RTQPHQSCAQ QGPERVEPDD WTSTLMSRGR NRQPLVLGDN VFADLVGNWL DLPELEKGGE
KGETGGAREP KGEKGQPQEL GRRFALTANI FKKFLRSVRP DRDRLLKEKP GWVTPMVPES
RTGRSQKVKK RSLSKGSGHF PFPGTGEHRR GENPPTSCPK ALEHSPSGFD INTAVWV
