INKA2_MOUSE
ID INKA2_MOUSE Reviewed; 300 AA.
AC Q80VY2; Q8C5H0; Q8CCA1;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=PAK4-inhibitor INKA2 {ECO:0000305};
DE AltName: Full=Induced in neural crest by AP2-alpha protein-related homolog {ECO:0000303|PubMed:17314132};
DE Short=MInca-r {ECO:0000303|PubMed:17314132};
GN Name=Inka2 {ECO:0000312|MGI:MGI:1923497}; Synonyms=Fam212b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Colon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=17314132; DOI=10.1242/dev.02813;
RA Luo T., Xu Y., Hoffman T.L., Zhang T., Schilling T., Sargent T.D.;
RT "Inca: a novel p21-activated kinase-associated protein required for cranial
RT neural crest development.";
RL Development 134:1279-1289(2007).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=26292052; DOI=10.1016/j.gep.2015.08.002;
RA Iwasaki Y., Yumoto T., Sakakibara S.;
RT "Expression profiles of inka2 in the murine nervous system.";
RL Gene Expr. Patterns 19:83-97(2015).
CC -!- FUNCTION: Inhibitor of the serine/threonine-protein kinase PAK4. Acts
CC by binding PAK4 in a substrate-like manner, inhibiting the protein
CC kinase activity. {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- SUBUNIT: Interacts with PAK4. {ECO:0000250|UniProtKB:Q9NTI7}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NTI7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80VY2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80VY2-2; Sequence=VSP_028174;
CC -!- TISSUE SPECIFICITY: Enriched in the nervous system.
CC {ECO:0000269|PubMed:26292052}.
CC -!- DEVELOPMENTAL STAGE: During development, expressed in oligodendrocyte
CC progenitor cells and in the proliferative neuronal progenitors in the
CC developing cerebellum. In the embryonic brain, not expressed in
CC immature newborn neurons, except for the cells residing in the marginal
CC zone of the embryonic telencephalon. As brain development proceeds
CC during the postnatal stage, expressed in some populations of immature
CC neurons, including the neocortical pyramidal neurons, hippocampal
CC pyramidal neurons and granule cells migrating in the cerebellar cortex.
CC In the adult brain, expression is confined in terminally differentiated
CC neurons in the restricted forebrain regions.
CC {ECO:0000269|PubMed:26292052}.
CC -!- DOMAIN: The Inka box (also named iBox or inca box) binds and inhibits
CC PAK4 by binding a substrate-like manner.
CC {ECO:0000250|UniProtKB:Q96EL1}.
CC -!- SIMILARITY: Belongs to the INKA family. {ECO:0000305}.
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DR EMBL; AK033558; BAC28358.1; -; mRNA.
DR EMBL; AK078368; BAC37240.1; -; mRNA.
DR EMBL; BC052371; AAH52371.1; -; mRNA.
DR CCDS; CCDS17710.1; -. [Q80VY2-1]
DR CCDS; CCDS51035.1; -. [Q80VY2-2]
DR RefSeq; NP_001156828.1; NM_001163356.1. [Q80VY2-2]
DR RefSeq; NP_780607.2; NM_175398.4. [Q80VY2-1]
DR AlphaFoldDB; Q80VY2; -.
DR SMR; Q80VY2; -.
DR BioGRID; 224536; 1.
DR STRING; 10090.ENSMUSP00000069212; -.
DR PhosphoSitePlus; Q80VY2; -.
DR PaxDb; Q80VY2; -.
DR PeptideAtlas; Q80VY2; -.
DR PRIDE; Q80VY2; -.
DR ProteomicsDB; 267140; -. [Q80VY2-1]
DR ProteomicsDB; 267141; -. [Q80VY2-2]
DR Antibodypedia; 33813; 123 antibodies from 13 providers.
DR Ensembl; ENSMUST00000066610; ENSMUSP00000069212; ENSMUSG00000048458. [Q80VY2-1]
DR Ensembl; ENSMUST00000098273; ENSMUSP00000095874; ENSMUSG00000048458. [Q80VY2-2]
DR GeneID; 109050; -.
DR KEGG; mmu:109050; -.
DR UCSC; uc008qvc.2; mouse. [Q80VY2-1]
DR CTD; 55924; -.
DR MGI; MGI:1923497; Inka2.
DR VEuPathDB; HostDB:ENSMUSG00000048458; -.
DR eggNOG; ENOG502RZNT; Eukaryota.
DR GeneTree; ENSGT00530000063849; -.
DR HOGENOM; CLU_082435_0_0_1; -.
DR InParanoid; Q80VY2; -.
DR OMA; DTIDYTQ; -.
DR OrthoDB; 794088at2759; -.
DR PhylomeDB; Q80VY2; -.
DR TreeFam; TF332839; -.
DR BioGRID-ORCS; 109050; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Inka2; mouse.
DR PRO; PR:Q80VY2; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q80VY2; protein.
DR Bgee; ENSMUSG00000048458; Expressed in retinal neural layer and 126 other tissues.
DR Genevisible; Q80VY2; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; ISO:MGI.
DR GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; ISS:UniProtKB.
DR InterPro; IPR029267; FAM212.
DR InterPro; IPR039201; Inka.
DR PANTHER; PTHR28615; PTHR28615; 1.
DR Pfam; PF15342; FAM212; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Nucleus; Reference proteome.
FT CHAIN 1..300
FT /note="PAK4-inhibitor INKA2"
FT /id="PRO_0000304995"
FT REGION 59..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 140..183
FT /note="Inka box"
FT /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT REGION 178..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..19
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028174"
FT CONFLICT 195
FT /note="G -> R (in Ref. 1; BAC28358)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 33233 MW; 6A2006152573C324 CRC64;
MTKESKDMDC YLRRLKQELM SMKEVGDGLQ DQMNCMMGAL QELKLLQVQT ALEQLEISGG
TPTFSCPESS QEQPECPRWQ GSGGPAGPAA WTSSSQPSFD SSPKLPCRRS VCGKELAVLP
KTQLPEEHQS CTQQGTEWVE PDDWTSTLMS RGRNRQPLVL GDNVFADLVG NWLDLPELEK
GGEKGETGGS IEPKGEKGQS RELGRKFALT ANIFRKFLRS VRPDRDRLLK EKPGWMTPMV
SESRAGRSKK VKKRSLSKGS GRFPFSSTGE PRHIETPATS SPKALEPSCR GFDINTAVWV
