ID   INO1_HORVU              Reviewed;         510 AA.
AC   O65195;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Inositol-3-phosphate synthase;
DE            Short=MIP synthase;
DE            EC=5.5.1.4;
DE   AltName: Full=Myo-inositol 1-phosphate synthase;
DE            Short=IPS;
DE            Short=MI-1-P synthase;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Harrington;
RA   Larson S.R., Raboy V.;
RT   "Linkage mapping maize and barley myo-inositol 1-phosphate synthase
RT   genes.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate;
CC         Xref=Rhea:RHEA:10716, ChEBI:CHEBI:58401, ChEBI:CHEBI:61548;
CC         EC=5.5.1.4;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Polyol metabolism; myo-inositol biosynthesis; myo-inositol
CC       from D-glucose 6-phosphate: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the myo-inositol 1-phosphate synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AF056325; AAC17133.1; -; mRNA.
DR   PIR; T04399; T04399.
DR   AlphaFoldDB; O65195; -.
DR   SMR; O65195; -.
DR   IntAct; O65195; 1.
DR   UniPathway; UPA00823; UER00787.
DR   ExpressionAtlas; O65195; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004512; F:inositol-3-phosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006021; P:inositol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002587; Myo-inos-1-P_Synthase.
DR   InterPro; IPR013021; Myo-inos-1-P_Synthase_GAPDH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR11510; PTHR11510; 1.
DR   Pfam; PF01658; Inos-1-P_synth; 1.
DR   Pfam; PF07994; NAD_binding_5; 1.
DR   PIRSF; PIRSF015578; Myoinos-ppht_syn; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Inositol biosynthesis; Isomerase; Lipid biosynthesis;
KW   Lipid metabolism; NAD; Phospholipid biosynthesis; Phospholipid metabolism.
FT   CHAIN           1..510
FT                   /note="Inositol-3-phosphate synthase"
FT                   /id="PRO_0000195191"
SQ   SEQUENCE   510 AA;  56173 MW;  EA63138121692724 CRC64;
     MFIESFRVES PKVRYGAGEI ESEYRYDTTE LVHESHDGAS KWVVRPKSVN YHFKTNTTVP
     KLGVMLVGWG GNNGSTLMAG VIANREGISW ATKDKVQQAN YFGSLTQAST IRVGSYNGEE
     IYAPFKSLLP MVNPDDLVFG GWDISSMNLA DAMTRAKVLD IDLQKQLRPY MESMCPLPGI
     YDPDFIAANQ GSRANNVIKG TKKEQMEQVI KDIREFKEKN KVDKVVVLWT ANTERYSNVS
     VGLNDTTENL LASVDKNEAE ISPSTLYAIA CVMEGVPFIN GSPQNTFVPG LIDLAIKNDC
     LIGGDDFKSG QTKMKSVLVD FLVGAGIKPT SIVSYNHLGN NDGMNLSAPQ TFRSKEISKS
     NVVDDMVSSN AILYEPGEHP DHVVVIKYVP YVGDSKRAMD EYTSEIFMGG KSTIVLHNTC
     EDSLLRAPII LDLVLLAELS TRIQLKAEGE DKLHSFHPVA TILSYLTKAP LVPPGTPVVN
     ALAKQRAMLE NIMRACVGLA PENNMILEYK