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INO2_ARATH
ID   INO2_ARATH              Reviewed;         510 AA.
AC   Q38862; Q9SIE2;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2001, sequence version 2.
DT   03-AUG-2022, entry version 159.
DE   RecName: Full=Inositol-3-phosphate synthase isozyme 2;
DE            Short=AtIPS2;
DE            Short=MIP synthase 2;
DE            EC=5.5.1.4;
DE   AltName: Full=Myo-inositol 1-phosphate synthase 2;
DE            Short=AtMIPS 2;
DE            Short=MI-1-P synthase 2;
GN   Name=IPS2; Synonyms=MIPS2; OrderedLocusNames=At2g22240; ORFNames=T26C19.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RA   Johnson M.D., Burk D.H.;
RT   "Isozyme of 1L-myo-inositol1-phosphate synthase from Arabidopsis.";
RL   (er) Plant Gene Register PGR95-067(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=18603618; DOI=10.1093/jxb/ern161;
RA   Mitsuhashi N., Kondo M., Nakaune S., Ohnishi M., Hayashi M.,
RA   Hara-Nishimura I., Richardson A., Fukaki H., Nishimura M., Mimura T.;
RT   "Localization of myo-inositol-1-phosphate synthase to the endosperm in
RT   developing seeds of Arabidopsis.";
RL   J. Exp. Bot. 59:3069-3076(2008).
RN   [6]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=18643983; DOI=10.1111/j.1365-313x.2008.03629.x;
RA   Murphy A.M., Otto B., Brearley C.A., Carr J.P., Hanke D.E.;
RT   "A role for inositol hexakisphosphate in the maintenance of basal
RT   resistance to plant pathogens.";
RL   Plant J. 56:638-652(2008).
RN   [7]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=19812700; DOI=10.1371/journal.pone.0007364;
RA   Meng P.H., Raynaud C., Tcherkez G., Blanchet S., Massoud K.,
RA   Domenichini S., Henry Y., Soubigou-Taconnat L., Lelarge-Trouverie C.,
RA   Saindrenan P., Renou J.P., Bergounioux C.;
RT   "Crosstalks between myo-inositol metabolism, programmed cell death and
RT   basal immunity in Arabidopsis.";
RL   PLoS ONE 4:E7364-E7364(2009).
RN   [8]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP   SPECIFICITY, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX   PubMed=20215587; DOI=10.1105/tpc.109.071779;
RA   Donahue J.L., Alford S.R., Torabinejad J., Kerwin R.E., Nourbakhsh A.,
RA   Ray W.K., Hernick M., Huang X., Lyons B.M., Hein P.P., Gillaspy G.E.;
RT   "The Arabidopsis thaliana Myo-inositol 1-phosphate synthase1 gene is
RT   required for Myo-inositol synthesis and suppression of cell death.";
RL   Plant Cell 22:888-903(2010).
CC   -!- FUNCTION: Involved in myo-inositol synthesis.
CC       {ECO:0000269|PubMed:20215587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate;
CC         Xref=Rhea:RHEA:10716, ChEBI:CHEBI:58401, ChEBI:CHEBI:61548;
CC         EC=5.5.1.4; Evidence={ECO:0000269|PubMed:20215587};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.45 mM for D-glucose 6-phosphate {ECO:0000269|PubMed:20215587};
CC         KM=0.30 uM for NAD(+) {ECO:0000269|PubMed:20215587};
CC         Note=kcat is 4.0 min(-1) for D-glucose 6-phosphate. kcat is 3.6 min(-
CC         1) for NAD(+).;
CC   -!- PATHWAY: Polyol metabolism; myo-inositol biosynthesis; myo-inositol
CC       from D-glucose 6-phosphate: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18603618,
CC       ECO:0000269|PubMed:20215587}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q38862-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Expressed in siliques, leaves, roots, seed
CC       endosperm, but not in embryos. Highest expression in seeds. In leaves,
CC       only expressed in hydathodes and vascular tissue.
CC       {ECO:0000269|PubMed:18603618, ECO:0000269|PubMed:20215587}.
CC   -!- DISRUPTION PHENOTYPE: No spontaneous lesion formation and no
CC       significant alteration in the response to abscisic acid. Increased
CC       susceptibility to bacterial and fungal pathogens.
CC       {ECO:0000269|PubMed:18643983, ECO:0000269|PubMed:19812700,
CC       ECO:0000269|PubMed:20215587}.
CC   -!- SIMILARITY: Belongs to the myo-inositol 1-phosphate synthase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Was called MIPS1 in PubMed:18603618. {ECO:0000305}.
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DR   EMBL; U30250; AAC49172.1; -; mRNA.
DR   EMBL; AC007168; AAD23618.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07280.1; -; Genomic_DNA.
DR   EMBL; AY053415; AAK96645.1; -; mRNA.
DR   EMBL; AY054202; AAL06863.1; -; mRNA.
DR   EMBL; AY143904; AAN28843.1; -; mRNA.
DR   PIR; D84610; D84610.
DR   RefSeq; NP_179812.1; NM_127790.4. [Q38862-1]
DR   AlphaFoldDB; Q38862; -.
DR   SMR; Q38862; -.
DR   BioGRID; 2110; 2.
DR   IntAct; Q38862; 2.
DR   STRING; 3702.AT2G22240.1; -.
DR   PaxDb; Q38862; -.
DR   PRIDE; Q38862; -.
DR   EnsemblPlants; AT2G22240.1; AT2G22240.1; AT2G22240. [Q38862-1]
DR   GeneID; 816757; -.
DR   Gramene; AT2G22240.1; AT2G22240.1; AT2G22240. [Q38862-1]
DR   KEGG; ath:AT2G22240; -.
DR   Araport; AT2G22240; -.
DR   TAIR; locus:2060364; AT2G22240.
DR   eggNOG; KOG0693; Eukaryota.
DR   HOGENOM; CLU_021486_2_0_1; -.
DR   InParanoid; Q38862; -.
DR   OrthoDB; 451916at2759; -.
DR   PhylomeDB; Q38862; -.
DR   BioCyc; ARA:AT2G22240-MON; -.
DR   BRENDA; 5.5.1.4; 399.
DR   SABIO-RK; Q38862; -.
DR   UniPathway; UPA00823; UER00787.
DR   PRO; PR:Q38862; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q38862; baseline and differential.
DR   Genevisible; Q38862; AT.
DR   GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR   GO; GO:0004512; F:inositol-3-phosphate synthase activity; IGI:TAIR.
DR   GO; GO:0016036; P:cellular response to phosphate starvation; TAS:TAIR.
DR   GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR   GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR   GO; GO:0051607; P:defense response to virus; IMP:TAIR.
DR   GO; GO:0009793; P:embryo development ending in seed dormancy; IGI:TAIR.
DR   GO; GO:0006021; P:inositol biosynthetic process; IBA:GO_Central.
DR   GO; GO:0010264; P:myo-inositol hexakisphosphate biosynthetic process; IMP:TAIR.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009733; P:response to auxin; TAS:TAIR.
DR   InterPro; IPR002587; Myo-inos-1-P_Synthase.
DR   InterPro; IPR013021; Myo-inos-1-P_Synthase_GAPDH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR11510; PTHR11510; 1.
DR   Pfam; PF01658; Inos-1-P_synth; 1.
DR   Pfam; PF07994; NAD_binding_5; 1.
DR   PIRSF; PIRSF015578; Myoinos-ppht_syn; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Inositol biosynthesis; Isomerase;
KW   Lipid biosynthesis; Lipid metabolism; NAD; Phospholipid biosynthesis;
KW   Phospholipid metabolism; Reference proteome.
FT   CHAIN           1..510
FT                   /note="Inositol-3-phosphate synthase isozyme 2"
FT                   /id="PRO_0000195187"
FT   CONFLICT        135
FT                   /note="E -> D (in Ref. 1; AAC49172)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        287
FT                   /note="F -> L (in Ref. 1; AAC49172)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        298
FT                   /note="K -> N (in Ref. 1; AAC49172)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        318
FT                   /note="L -> W (in Ref. 1; AAC49172)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        477
FT                   /note="P -> A (in Ref. 1; AAC49172)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        487
FT                   /note="A -> P (in Ref. 1; AAC49172)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   510 AA;  56337 MW;  BDD58C166E289CE5 CRC64;
     MFIESFKVES PNVKYTENEI NSVYDYETTE VVHENRNGTY QWVVKPKTVK YDFKTDTRVP
     KLGVMLVGWG GNNGSTLTAG VIANKEGISW ATKDKVQQAN YFGSLTQASS IRVGSYNGEE
     IYAPFKSLLP MVNPEDVVFG GWDISDMNLA DAMARARVLD IDLQKQLRPY MENMIPLPGI
     YDPDFIAANQ GSRANSVIKG TKKEQVDHII KDMREFKEKN KVDKLVVLWT ANTERYSNVI
     VGLNDTTENL LASVEKDESE ISPSTLYAIA CVLEGIPFIN GSPQNTFVPG LIELAISKNC
     LIGGDDFKSG QTKMKSVLVD FLVGAGIKPT SIVSYNHLGN NDGMNLSAPQ TFRSKEISKS
     NVVDDMVASN GILFEPGEHP DHVVVIKYVP YVADSKRAMD EYTSEIFMGG RNTIVLHNTC
     EDSLLAAPII LDLVLLAELS TRIQFKAEGE GKFHSFHPVA TILSYLTKAP LVPPGTPVVN
     ALSKQRAMLE NILRACVGLA PENNMIMEYK
 
 
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