ID   INP1_ARATH              Reviewed;         273 AA.
AC   Q9SUV9; A0MF90;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Protein INAPERTURATE POLLEN1 {ECO:0000303|PubMed:23136373};
GN   Name=INP1 {ECO:0000303|PubMed:23136373};
GN   OrderedLocusNames=At4g22600 {ECO:0000312|Araport:AT4G22600};
GN   ORFNames=F7K2.180 {ECO:0000312|EMBL:CAA22165.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=23136373; DOI=10.1105/tpc.112.101220;
RA   Dobritsa A.A., Coerper D.;
RT   "The novel plant protein INAPERTURATE POLLEN1 marks distinct cellular
RT   domains and controls formation of apertures in the Arabidopsis pollen
RT   exine.";
RL   Plant Cell 24:4452-4464(2012).
RN   [5]
RP   FUNCTION, AND MISCELLANEOUS.
RX   PubMed=27177036; DOI=10.1371/journal.pgen.1006060;
RA   Reeder S.H., Lee B.H., Fox R., Dobritsa A.A.;
RT   "A ploidy-sensitive mechanism regulates aperture formation on the
RT   Arabidopsis pollen surface and guides localization of the aperture factor
RT   INP1.";
RL   PLoS Genet. 12:E1006060-E1006060(2016).
CC   -!- FUNCTION: Required for the formation of pollen surface apertures, which
CC       arise by restriction of exine deposition at specific sites. The
CC       aperture length depends on the INP1 dosage (PubMed:23136373). Does not
CC       play a role in specifying the number or position of apertures
CC       (PubMed:27177036). Acts in a sporophytic manner (PubMed:23136373).
CC       {ECO:0000269|PubMed:23136373, ECO:0000269|PubMed:27177036}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23136373}.
CC       Note=Located at 3 equidistant spots close to the pollen equator. 3-D
CC       reconstruction demonstrates that INP1 is restricted not to just 3
CC       spots, but to 3 long and narrow regions underlying the position of
CC       future apertures. {ECO:0000269|PubMed:23136373}.
CC   -!- TISSUE SPECIFICITY: Expressed only in anthers and in pollen. Not
CC       detected in other flower tissues, stems, leaves and siliques.
CC       {ECO:0000269|PubMed:23136373}.
CC   -!- DEVELOPMENTAL STAGE: Detected in stage 9 buds and appears to be
CC       confined to microspores. {ECO:0000269|PubMed:23136373}.
CC   -!- DISRUPTION PHENOTYPE: Normal and fertile plants with pollen having
CC       normal reticulate exine patterning but completely lacking apertures.
CC       {ECO:0000269|PubMed:23136373}.
CC   -!- MISCELLANEOUS: The number and position of apertures is strongly
CC       correlated with pollen ploidy. {ECO:0000269|PubMed:27177036}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK28257.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL033545; CAA22165.1; -; Genomic_DNA.
DR   EMBL; AL161557; CAB79215.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE84629.1; -; Genomic_DNA.
DR   EMBL; DQ459193; ABE97191.1; -; Genomic_DNA.
DR   EMBL; DQ653216; ABK28257.1; ALT_SEQ; Genomic_DNA.
DR   PIR; T05454; T05454.
DR   RefSeq; NP_193991.1; NM_118386.1.
DR   AlphaFoldDB; Q9SUV9; -.
DR   STRING; 3702.AT4G22600.1; -.
DR   PaxDb; Q9SUV9; -.
DR   PRIDE; Q9SUV9; -.
DR   EnsemblPlants; AT4G22600.1; AT4G22600.1; AT4G22600.
DR   GeneID; 828356; -.
DR   Gramene; AT4G22600.1; AT4G22600.1; AT4G22600.
DR   KEGG; ath:AT4G22600; -.
DR   Araport; AT4G22600; -.
DR   TAIR; locus:2127525; AT4G22600.
DR   eggNOG; ENOG502R2CB; Eukaryota.
DR   HOGENOM; CLU_1063214_0_0_1; -.
DR   InParanoid; Q9SUV9; -.
DR   OMA; QFLVGFR; -.
DR   OrthoDB; 1499204at2759; -.
DR   PhylomeDB; Q9SUV9; -.
DR   PRO; PR:Q9SUV9; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9SUV9; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0010584; P:pollen exine formation; IMP:TAIR.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR025422; TGA_domain.
DR   Pfam; PF14144; DOG1; 1.
DR   PROSITE; PS51806; DOG1; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..273
FT                   /note="Protein INAPERTURATE POLLEN1"
FT                   /id="PRO_0000437207"
FT   DOMAIN          12..267
FT                   /note="DOG1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
SQ   SEQUENCE   273 AA;  31249 MW;  7E644F686CE8EBB0 CRC64;
     MPFSFFSRKK PSRRFNDFYE DWSKTLTENC LPLLRQSLSS AASASVLSSN VDLVLRHLVL
     YYETLDLAAD HNTIPYLLFP SWRNSLETPF LFLGDIHPYL LTNLLRSFID RENQDSDDED
     EETSLDLVNQ PLKMTMAWKD PSDELVKRID QIECTMRLMV PGLMDRMRKT QRSFVARVSE
     SWVSSYQVGK KKKLTATVAT ASTSVDEAAK IEMEELVSIF VDANRLRKSV IMDIVGATSE
     HQAALFLEGL CQFLAGFKDQ ILLQDFEILS LPN