INP1_ORYSJ
ID INP1_ORYSJ Reviewed; 232 AA.
AC Q6H6M5; Q6H694;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2020, sequence version 1.
DT 25-MAY-2022, entry version 9.
DE RecName: Full=Protein INAPERTURATE POLLEN 1 homolog {ECO:0000305};
DE Short=OsINP1 {ECO:0000303|PubMed:32284546};
GN Name=INP1 {ECO:0000303|PubMed:32284546};
GN OrderedLocusNames=Os02g0661300 {ECO:0000312|EMBL:BAF09560.1},
GN LOC_Os02g44250 {ECO:0000305};
GN ORFNames=P0516F12.11 {ECO:0000312|EMBL:BAD25624.1},
GN P0708H12.37 {ECO:0000312|EMBL:BAD25755.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP FUNCTION, INTERACTION WITH LECRKS7/DAF1, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=32284546; DOI=10.1038/s41477-020-0630-6;
RA Zhang X., Zhao G., Tan Q., Yuan H., Betts N., Zhu L., Zhang D., Liang W.;
RT "Rice pollen aperture formation is regulated by the interplay between
RT OsINP1 and OsDAF1.";
RL Nat. Plants 6:394-403(2020).
CC -!- FUNCTION: Required for pollen aperture formation, male fertility and
CC LECRKS7/DAF1 function (PubMed:32284546). Seems to be involved in
CC operculum protrusion (PubMed:32284546). Participates in the
CC modification of plasma membrane at future aperture sites, possibly by
CC creating close contact between the plasma membrane and callose wall to
CC prevent primexine formation and sporopollenin deposition
CC (PubMed:32284546). {ECO:0000269|PubMed:32284546}.
CC -!- SUBUNIT: Interacts with LECRKS7/DAF1. {ECO:0000269|PubMed:32284546}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:32284546}.
CC Note=Localizes uniformly in the cytoplasm of microspore mother cells
CC (MMCs) during meiosis, in dyads and in the early tetrad stage. In the
CC late tetrad stage, starts to move to distal poles of the tetrad,
CC concentrating into prominent spots. Finally, localizes specifically at
CC the site that marks the future aperture. {ECO:0000269|PubMed:32284546}.
CC -!- DISRUPTION PHENOTYPE: Absence of entire pollen aperture, leading to
CC male sterility. {ECO:0000269|PubMed:32284546}.
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DR EMBL; AP004883; BAD25624.1; -; Genomic_DNA.
DR EMBL; AP005072; BAD25755.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09560.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS80142.1; -; Genomic_DNA.
DR EMBL; AK106503; BAG97742.1; -; mRNA.
DR AlphaFoldDB; Q6H6M5; -.
DR SMR; Q6H6M5; -.
DR STRING; 4530.OS02T0661300-01; -.
DR EnsemblPlants; Os02t0661300-01; Os02t0661300-01; Os02g0661300.
DR Gramene; Os02t0661300-01; Os02t0661300-01; Os02g0661300.
DR eggNOG; ENOG502R2CB; Eukaryota.
DR HOGENOM; CLU_1063214_0_0_1; -.
DR OMA; QFLVGFR; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0062074; C:pollen aperture; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0062075; P:pollen aperture formation; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR025422; TGA_domain.
DR Pfam; PF14144; DOG1; 1.
DR PROSITE; PS51806; DOG1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..232
FT /note="Protein INAPERTURATE POLLEN 1 homolog"
FT /id="PRO_0000450664"
SQ SEQUENCE 232 AA; 25029 MW; B7B852BD67262EAF CRC64;
MPRPPPPPPP GRGAPGARRP MREFFAAWLS TLRSPLLPLL RRALSSSSSS SSGGWDDPLS
SAAAAVEAHF QAHWSALDAA ARQDPAQAVS AGDWRSPLEL PFLWVGDLHP SLVTSLLRSL
SPSPRLLAAT DRVDRRIRAA VPSISDRLRR VQEAFISAEV SGAADVEAFL EELKDVALDA
NRLRRGVLSE LVAAAGGYQA ALFLEALSRF VLSMHDPEVL RRFDQCRASP GS
