INS1A_DANRE
ID INS1A_DANRE Reviewed; 383 AA.
AC Q6P0F9; Q3S5C8;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Insulinoma-associated protein 1a;
DE AltName: Full=Insulinoma-associated 1-like protein a;
DE AltName: Full=Zinc finger protein IA-1a;
GN Name=insm1a;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AB;
RX PubMed=16487754; DOI=10.1016/j.modgep.2005.12.008;
RA Lukowski C.M., Ritzel R.G., Waskiewicz A.J.;
RT "Expression of two insm1-like genes in the developing zebrafish nervous
RT system.";
RL Gene Expr. Patterns 6:711-718(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May act as a transcriptional regulator. May play a role in
CC neurogenesis and neuroendocrine cell differentiation during embryonic
CC development (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q63ZV0}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expressed at 10 hours post-fertilization (hpf), reach peak levels at 48
CC hpf and is not detectable in adult tissues. Expressed during
CC neurogenesis between 9 and 72 hpf and in ventral endoderm region where
CC pancreatic progenitors originate. {ECO:0000269|PubMed:16487754}.
CC -!- SIMILARITY: Belongs to the INSM1 family. {ECO:0000305}.
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DR EMBL; DQ164184; AAZ94623.1; -; mRNA.
DR EMBL; CABZ01008096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CU694316; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC065636; AAH65636.1; -; mRNA.
DR RefSeq; NP_991207.1; NM_205644.1.
DR AlphaFoldDB; Q6P0F9; -.
DR STRING; 7955.ENSDARP00000107863; -.
DR PaxDb; Q6P0F9; -.
DR Ensembl; ENSDART00000124040; ENSDARP00000107863; ENSDARG00000091756.
DR GeneID; 402941; -.
DR KEGG; dre:402941; -.
DR CTD; 402941; -.
DR ZFIN; ZDB-GENE-040426-1810; insm1a.
DR eggNOG; KOG3993; Eukaryota.
DR GeneTree; ENSGT00940000162552; -.
DR HOGENOM; CLU_033476_1_0_1; -.
DR InParanoid; Q6P0F9; -.
DR OrthoDB; 1306883at2759; -.
DR PhylomeDB; Q6P0F9; -.
DR TreeFam; TF320538; -.
DR PRO; PR:Q6P0F9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR Bgee; ENSDARG00000091756; Expressed in camera-type eye and 42 other tissues.
DR ExpressionAtlas; Q6P0F9; baseline.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0017053; C:transcription repressor complex; ISS:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:ZFIN.
DR GO; GO:0043697; P:cell dedifferentiation; IMP:ZFIN.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0003310; P:pancreatic A cell differentiation; ISS:UniProtKB.
DR GO; GO:0035677; P:posterior lateral line neuromast hair cell development; IMP:ZFIN.
DR GO; GO:0048920; P:posterior lateral line neuromast primordium migration; IMP:ZFIN.
DR GO; GO:0010564; P:regulation of cell cycle process; IMP:ZFIN.
DR GO; GO:0042670; P:retinal cone cell differentiation; IMP:ZFIN.
DR GO; GO:0060221; P:retinal rod cell differentiation; IMP:ZFIN.
DR GO; GO:0070654; P:sensory epithelium regeneration; IGI:ZFIN.
DR GO; GO:0021522; P:spinal cord motor neuron differentiation; IMP:ZFIN.
DR GO; GO:0060290; P:transdifferentiation; ISS:UniProtKB.
DR GO; GO:0003309; P:type B pancreatic cell differentiation; ISS:UniProtKB.
DR InterPro; IPR042972; INSM1/2.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR15065; PTHR15065; 1.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW Neurogenesis; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..383
FT /note="Insulinoma-associated protein 1a"
FT /id="PRO_0000425454"
FT ZN_FING 209..231
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 271..295
FT /note="C2H2-type 2; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 314..337
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 342..365
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..20
FT /note="SNAG domain"
FT /evidence="ECO:0000250|UniProtKB:Q63ZV0"
FT REGION 99..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 178
FT /note="L -> P (in Ref. 1; AAZ94623)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 383 AA; 42875 MW; A12D189EB5DE7C59 CRC64;
MPRGFLVKRN KKATPVSYRV RSEEDEQGAF VAQDVPCARR PVSPVQFGNP ETVYRAMYSP
TRPVSREHER ACLERRFNLG SPISAESFPA APNCSDQAPV DLKIGTSNSN RTGTTVTTKR
PASDTERKGK PASKKAKAMR KLQFEDEMTT SPVLGLKIKE GPVEQKPRSQ CASGDKPLGE
FVCQLCREAY ADPFSLAQHK CSRIVRIEYR CPECDKLFSC PANLASHRRW HKPKQSAESN
KTPAPEKEET SSDRDTPSPG LSESGSEDGL YDCQHCGKKF KRQAYLKKHV TAHHDAPEKP
QSHAPLNLSA SECHLCPVCG ENFPSRMSQE RHIRLQHSAQ VYPCKYCPAM FYSSPGLTRH
INKCHPSENR QVILLQMPVR PAC
