INS1B_DANRE
ID INS1B_DANRE Reviewed; 453 AA.
AC Q7T3H2; Q3S5C7;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Insulinoma-associated protein 1b;
DE AltName: Full=Insulinoma-associated 1-like protein b;
DE AltName: Full=Zinc finger protein IA-1b;
GN Name=insm1b;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AB;
RX PubMed=16487754; DOI=10.1016/j.modgep.2005.12.008;
RA Lukowski C.M., Ritzel R.G., Waskiewicz A.J.;
RT "Expression of two insm1-like genes in the developing zebrafish nervous
RT system.";
RL Gene Expr. Patterns 6:711-718(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May act as a transcriptional regulator. May play a role in
CC neurogenesis and neuroendocrine cell differentiation during embryonic
CC development (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q63ZV0}.
CC -!- DEVELOPMENTAL STAGE: Expressed at 10 hours post-fertilization (hpf),
CC reach peak levels at 48 hpf and is not detectable in adult tissues.
CC Expressed during neurogenesis between 9 and 72 hpf and in ventral
CC endoderm region where pancreatic progenitors originate.
CC {ECO:0000269|PubMed:16487754}.
CC -!- SIMILARITY: Belongs to the INSM1 family. {ECO:0000305}.
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DR EMBL; DQ164185; AAZ94624.1; -; mRNA.
DR EMBL; CR381689; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC053119; AAH53119.1; -; mRNA.
DR RefSeq; NP_955952.1; NM_199658.1.
DR AlphaFoldDB; Q7T3H2; -.
DR STRING; 7955.ENSDARP00000069814; -.
DR PaxDb; Q7T3H2; -.
DR PRIDE; Q7T3H2; -.
DR Ensembl; ENSDART00000075331; ENSDARP00000069814; ENSDARG00000053301.
DR GeneID; 323882; -.
DR KEGG; dre:323882; -.
DR CTD; 323882; -.
DR ZFIN; ZDB-GENE-030131-2602; insm1b.
DR eggNOG; KOG3993; Eukaryota.
DR GeneTree; ENSGT00940000162552; -.
DR HOGENOM; CLU_033476_1_0_1; -.
DR InParanoid; Q7T3H2; -.
DR OMA; VFPCKHC; -.
DR OrthoDB; 1306883at2759; -.
DR PhylomeDB; Q7T3H2; -.
DR TreeFam; TF320538; -.
DR PRO; PR:Q7T3H2; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 17.
DR Bgee; ENSDARG00000053301; Expressed in larva and 39 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0017053; C:transcription repressor complex; ISS:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0003310; P:pancreatic A cell differentiation; ISS:UniProtKB.
DR GO; GO:0010564; P:regulation of cell cycle process; IBA:GO_Central.
DR GO; GO:0060290; P:transdifferentiation; ISS:UniProtKB.
DR GO; GO:0003309; P:type B pancreatic cell differentiation; ISS:UniProtKB.
DR InterPro; IPR042972; INSM1/2.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR15065; PTHR15065; 1.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW Neurogenesis; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..453
FT /note="Insulinoma-associated protein 1b"
FT /id="PRO_0000425455"
FT ZN_FING 252..274
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 321..343
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 383..406
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 412..435
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..20
FT /note="SNAG domain"
FT /evidence="ECO:0000250|UniProtKB:Q63ZV0"
FT REGION 140..179
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 163..179
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 16
FT /note="V -> I (in Ref. 1; AAZ94624)"
FT /evidence="ECO:0000305"
FT CONFLICT 156
FT /note="Q -> R (in Ref. 1; AAZ94624)"
FT /evidence="ECO:0000305"
FT CONFLICT 404
FT /note="L -> P (in Ref. 1; AAZ94624)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 453 AA; 49568 MW; 058C30B859760DB6 CRC64;
MPKGFLVKRN KKAALVSYRI RTDEDGGPTP ECPIAQIALS SPAPSASKPD SILLAFPSAG
AEAPVPVHKP VQFGNPEAVY QALYSPTRPV SKDHDRKYFE RSLNLGSPIS AESFPTPASL
TSLDHHLLFA PVDLKIGTSN SNRSGTASGA HAPAIQTGAK RPSADAAERK VSSKSAKKPK
AIRKLNFEDE VTTSPVLGLK IKEGPVDLKP RPSSGGTNKP LGEFICQLCK EEYSDPFSLA
QHKCSRIVRV EYRCPECEKV FSCPANLASH RRWHKPRVQS APKQALQPAK PFPEELRAEF
PSDRDTPSPG LSESGSEDGL YDCQHCGKRF KRQAYLRKHI LGHQALQNQI LGEAFRSAES
PDAMPSEDRQ SPAPLNLSPA DCLTCPACGE KLPNRASLER HLRLLHDDAQ AFPCKFCPAT
FYSSPGLTRH INKCHPTENR QVILLQMPVR NAC
