INS1_CONFO
ID INS1_CONFO Reviewed; 128 AA.
AC A0A0B5AC98;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 1.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=Con-Ins F1 {ECO:0000303|PubMed:25605914};
DE AltName: Full=Insulin 1 {ECO:0000312|EMBL:AJD85837.1};
DE Contains:
DE RecName: Full=Con-Ins F1 B chain {ECO:0000303|PubMed:25605914};
DE Contains:
DE RecName: Full=Con-Ins F1 A chain {ECO:0000303|PubMed:25605914};
DE Flags: Precursor;
OS Conus floridulus (Cone snail) (Lividoconus floridulus).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lividoconus.
OX NCBI_TaxID=97180;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND AMIDATION AT SER-127.
RC TISSUE=Venom gland;
RX PubMed=25605914; DOI=10.1073/pnas.1423857112;
RA Safavi-Hemami H., Gajewiak J., Karanth S., Robinson S.D., Ueberheide B.,
RA Douglass A.D., Schlegel A., Imperial J.S., Watkins M., Bandyopadhyay P.K.,
RA Yandell M., Li Q., Purcell A.W., Norton R.S., Ellgaard L., Olivera B.M.;
RT "Specialized insulin is used for chemical warfare by fish-hunting cone
RT snails.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:1743-1748(2015).
CC -!- FUNCTION: This venom insulin facilitates prey capture by rapidly
CC inducing hypoglycemic shock. Intraperitoneal injection of this peptide
CC into zebrafish lowers blood glucose with the same potency than human
CC insulin. In vivo, when applied to water, this peptide reduces overall
CC locomotor activity of zebrafish larvae, observed as a significant
CC decrease in the percentage of time spent swimming and movement
CC frequency. {ECO:0000250|UniProtKB:A0A0B5AC95}.
CC -!- SUBUNIT: Heterodimer of A and B chains; disulfide-linked.
CC {ECO:0000250|UniProtKB:A0A0B5AC95}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:A0A0B5AC95}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:25605914}.
CC -!- SIMILARITY: Belongs to the insulin family.
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DR EMBL; KP268605; AJD85837.1; -; mRNA.
DR AlphaFoldDB; A0A0B5AC98; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR016724; Insulin-rel_pep.
DR InterPro; IPR022353; Insulin_CS.
DR InterPro; IPR022352; Insulin_family.
DR Pfam; PF00049; Insulin; 1.
DR PIRSF; PIRSF018431; Molluscan_insulin_rel_peptide; 1.
DR PRINTS; PR00276; INSULINFAMLY.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 1: Evidence at protein level;
KW Amidation; Carbohydrate metabolism; Cleavage on pair of basic residues;
KW Disulfide bond; Gamma-carboxyglutamic acid; Glucose metabolism; Hormone;
KW Secreted; Signal; Toxin.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PEPTIDE 25..58
FT /note="Con-Ins F1 B chain"
FT /evidence="ECO:0000250|UniProtKB:A0A0B5ABD9"
FT /id="PRO_5002098284"
FT PROPEP 59..89
FT /note="C peptide"
FT /evidence="ECO:0000250|UniProtKB:A0A0B5ABD9"
FT /id="PRO_0000439332"
FT PEPTIDE 90..127
FT /note="Con-Ins F1 A chain"
FT /evidence="ECO:0000250|UniProtKB:A0A0B5ABD9"
FT /id="PRO_0000439333"
FT MOD_RES 115
FT /note="4-carboxyglutamate; partial"
FT /evidence="ECO:0000250|UniProtKB:A0A0B5AC95"
FT MOD_RES 127
FT /note="Serine amide"
FT /evidence="ECO:0000305|PubMed:25605914"
FT DISULFID 29..104
FT /evidence="ECO:0000305"
FT DISULFID 41..107
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250|UniProtKB:A0A0B5AC95"
FT DISULFID 53..120
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250|UniProtKB:A0A0B5AC95"
FT DISULFID 106..111
FT /evidence="ECO:0000250|UniProtKB:A0A0B5AC95"
SQ SEQUENCE 128 AA; 13900 MW; 36DD950703DC141F CRC64;
MTTSSYFLLV TLGLLLYVCR SSFGTEHTCE SDASPHPQGV CGSPLAEAVE AACELEEYLQ
GGTGKKRGRA SPLRKRRAFL SMLKARAKRN EASPLQRSGR GIVCECCKNH CNIEELTEYC
PPVTEGSG