ID   INSB1_ECOLI             Reviewed;         167 AA.
AC   P0CF25; P03830; P77707; Q2MCH3;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Insertion element IS1 1 protein InsB;
DE   AltName: Full=IS1a;
GN   Name=insB1; OrderedLocusNames=b0021, JW0020;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=375010; DOI=10.1007/bf00271673;
RA   Johnsrud L.;
RT   "DNA sequence of the transposable element IS1.";
RL   Mol. Gen. Genet. 169:213-218(1979).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=1849492; DOI=10.1016/0378-1119(91)90096-t;
RA   Umeda M., Ohtsubo E.;
RT   "Four types of IS1 with differences in nucleotide sequence reside in the
RT   Escherichia coli K-12 chromosome.";
RL   Gene 98:1-5(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA   Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA   Mizobuchi K., Nakata A.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT   2.4 min region.";
RL   Nucleic Acids Res. 20:3305-3308(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Absolutely required for transposition of IS1.
CC   -!- SIMILARITY: Belongs to the transposase 27 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA96229.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; J01729; AAA96229.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X52534; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U00096; AAC73132.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76033.1; -; Genomic_DNA.
DR   PIR; JN0135; JN0135.
DR   RefSeq; NP_414562.1; NC_000913.3.
DR   RefSeq; NP_416407.1; NC_000913.3.
DR   RefSeq; NP_417902.1; NC_000913.3.
DR   AlphaFoldDB; P0CF25; -.
DR   STRING; 511145.b0021; -.
DR   PaxDb; P0CF25; -.
DR   PRIDE; P0CF25; -.
DR   EnsemblBacteria; AAC73132; AAC73132; b0021.
DR   EnsemblBacteria; BAE76033; BAE76033; BAE76033.
DR   GeneID; 944743; -.
DR   GeneID; 945867; -.
DR   GeneID; 947949; -.
DR   KEGG; ecj:JW0020; -.
DR   KEGG; eco:b0021; -.
DR   KEGG; eco:b1893; -.
DR   KEGG; eco:b3445; -.
DR   EchoBASE; EB4700; -.
DR   eggNOG; COG1662; Bacteria.
DR   HOGENOM; CLU_076276_2_0_6; -.
DR   InParanoid; P0CF25; -.
DR   OMA; RTCARLW; -.
DR   PhylomeDB; P0CF25; -.
DR   BioCyc; EcoCyc:G7762-MON; -.
DR   PRO; PR:P0CF25; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   InterPro; IPR005063; Transposase_27.
DR   Pfam; PF03400; DDE_Tnp_IS1; 1.
PE   3: Inferred from homology;
KW   DNA recombination; Reference proteome; Transposable element; Transposition.
FT   CHAIN           1..167
FT                   /note="Insertion element IS1 1 protein InsB"
FT                   /id="PRO_0000075401"
SQ   SEQUENCE   167 AA;  19565 MW;  AE490018A2505589 CRC64;
     MPGNSPHYGR WPQHDFTSLK KLRPQSVTSR IQPGSDVIVC AEMDEQWGYV GAKSRQRWLF
     YAYDSLRKTV VAHVFGERTM ATLGRLMSLL SPFDVVIWMT DGWPLYESRL KGKLHVISKR
     YTQRIERHNL NLRQHLARLG RKSLSFSKSV ELHDKVIGHY LNIKHYQ