ID   INSB2_ECOLI             Reviewed;         167 AA.
AC   P0CF26; Q2MCE8; Q47302; Q47691;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Insertion element IS1 2 protein InsB;
DE   AltName: Full=IS1b;
GN   Name=insB2; OrderedLocusNames=b0264, JW0256;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=1849492; DOI=10.1016/0378-1119(91)90096-t;
RA   Umeda M., Ohtsubo E.;
RT   "Four types of IS1 with differences in nucleotide sequence reside in the
RT   Escherichia coli K-12 chromosome.";
RL   Gene 98:1-5(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8113168; DOI=10.1128/jb.176.5.1297-1302.1994;
RA   Volkert M.R., Loewen P.C., Switala J., Crowley D., Conley M.;
RT   "The delta (argF-lacZ)205(U169) deletion greatly enhances resistance to
RT   hydrogen peroxide in stationary-phase Escherichia coli.";
RL   J. Bacteriol. 176:1297-1302(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Absolutely required for transposition of IS1.
CC   -!- SIMILARITY: Belongs to the transposase 27 family. {ECO:0000305}.
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DR   EMBL; L20943; AAA69643.1; -; Unassigned_DNA.
DR   EMBL; U70214; AAB08695.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73367.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76050.1; -; Genomic_DNA.
DR   RefSeq; NP_414798.1; NC_000913.3.
DR   RefSeq; NP_414808.1; NC_000913.3.
DR   AlphaFoldDB; P0CF26; -.
DR   STRING; 511145.b0264; -.
DR   PaxDb; P0CF26; -.
DR   PRIDE; P0CF26; -.
DR   EnsemblBacteria; AAC73367; AAC73367; b0264.
DR   EnsemblBacteria; BAE76050; BAE76050; BAE76050.
DR   GeneID; 944950; -.
DR   GeneID; 947698; -.
DR   KEGG; ecj:JW0256; -.
DR   KEGG; eco:b0264; -.
DR   KEGG; eco:b0274; -.
DR   EchoBASE; EB4705; -.
DR   eggNOG; COG1662; Bacteria.
DR   HOGENOM; CLU_076276_2_0_6; -.
DR   InParanoid; P0CF26; -.
DR   OMA; AYLSAEC; -.
DR   PhylomeDB; P0CF26; -.
DR   BioCyc; EcoCyc:G6138-MON; -.
DR   PRO; PR:P0CF26; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IMP:EcoCyc.
DR   InterPro; IPR005063; Transposase_27.
DR   Pfam; PF03400; DDE_Tnp_IS1; 1.
PE   3: Inferred from homology;
KW   DNA recombination; Reference proteome; Transposable element; Transposition.
FT   CHAIN           1..167
FT                   /note="Insertion element IS1 2 protein InsB"
FT                   /id="PRO_0000075402"
SQ   SEQUENCE   167 AA;  19758 MW;  B1CA4E4035F49368 CRC64;
     MPGNRPHYGR WPQHDFPPFK KLRPQSVTSR IQPGSDVIVC AEMDEQWGYV GAKSRQRWLF
     YAYDRLRKTV VAHVFGERTM ATLGRLMSLL SPFDVVIWMT DGWPLYESRL KGKLHVISKR
     YTQRIERHNL NLRQHLARLG RKSLSFSKSV EQHDKVIGHY LNIKHYQ