ID   INSB8_ECOLI             Reviewed;         167 AA.
AC   P0CF30; P03830; P77707; Q2MCH3;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Insertion element IS1 8 protein InsB;
GN   OrderedLocusNames=JW2285;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Absolutely required for transposition of IS1.
CC   -!- SIMILARITY: Belongs to the transposase 27 family. {ECO:0000305}.
CC   -!- CAUTION: There is no equivalent of this gene in strain K12 / MG1655.
CC       {ECO:0000305}.
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DR   EMBL; AP009048; BAA16125.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0CF30; -.
DR   EnsemblBacteria; BAA16125; BAA16125; BAA16125.
DR   KEGG; ecj:JW2285; -.
DR   PATRIC; fig|83333.103.peg.1325; -.
DR   HOGENOM; CLU_076276_2_0_6; -.
DR   OMA; RTCARLW; -.
DR   PhylomeDB; P0CF30; -.
DR   PRO; PR:P0CF30; -.
DR   Proteomes; UP000000318; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   InterPro; IPR005063; Transposase_27.
DR   Pfam; PF03400; DDE_Tnp_IS1; 1.
PE   3: Inferred from homology;
KW   DNA recombination; Transposable element; Transposition.
FT   CHAIN           1..167
FT                   /note="Insertion element IS1 8 protein InsB"
FT                   /id="PRO_0000393405"
SQ   SEQUENCE   167 AA;  19565 MW;  AE490018A2505589 CRC64;
     MPGNSPHYGR WPQHDFTSLK KLRPQSVTSR IQPGSDVIVC AEMDEQWGYV GAKSRQRWLF
     YAYDSLRKTV VAHVFGERTM ATLGRLMSLL SPFDVVIWMT DGWPLYESRL KGKLHVISKR
     YTQRIERHNL NLRQHLARLG RKSLSFSKSV ELHDKVIGHY LNIKHYQ