ID   INSF_ECO11              Reviewed;         288 AA.
AC   P0CF85; O08009; O08012; O08304; P05822; P77673; Q2MBI8;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Transposase InsF for insertion sequence IS3;
GN   Name=insF;
OS   Escherichia coli O111:H-.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=168927;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O111ac:H- / 172 / EPEC;
RX   PubMed=9675859; DOI=10.1111/j.1574-6968.1998.tb13078.x;
RA   Sperandio V., Kaper J.B., Bortolini M.R., Neves B.C., Keller R.,
RA   Trabulsi L.R.;
RT   "Characterization of the locus of enterocyte effacement (LEE) in different
RT   enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing
RT   Escherichia coli (STEC) serotypes.";
RL   FEMS Microbiol. Lett. 164:133-139(1998).
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence IS3.
CC   -!- SIMILARITY: Belongs to the transposase IS3/IS150/IS904 family.
CC       {ECO:0000305}.
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DR   EMBL; AF041809; AAC28569.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0CF85; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR025948; HTH-like_dom.
DR   InterPro; IPR038965; InsF-like.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR46889:SF1; PTHR46889:SF1; 1.
DR   Pfam; PF13276; HTH_21; 1.
DR   Pfam; PF00665; rve; 1.
DR   Pfam; PF13333; rve_2; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Transposable element; Transposition.
FT   CHAIN           1..288
FT                   /note="Transposase InsF for insertion sequence IS3"
FT                   /id="PRO_0000394039"
FT   DOMAIN          124..287
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
SQ   SEQUENCE   288 AA;  33540 MW;  9237EA662E021EB4 CRC64;
     MKYVFIEKHQ AEFSIKAMCR VLRVARSGWY TWCQRRTRIS TRQQFRQHCD SVVLAAFTRS
     KQRYGAPRLT DELRAQGYPF NVKTVAASLR RQGLRAKASR KFSPVSYRAH GLPVSENLLE
     QDFYASGPNQ KWAGDITYLR TDEGWLYLAV VIDLWSRAVI GWSMSPRMTA QLACDALQMA
     LWRRKRPRNV IVHTDRGGQY CSADYQAQLK RHNLRGSMSA KGCCYDNACV ESFFHSLKVE
     CIHGEHFISR EIMRATVFNY IECDYNRWRR HSWCGGLSPE QFENKNLA