4HPE1_BURM1
ID 4HPE1_BURM1 Reviewed; 311 AA.
AC B3D6W2; A9AL52;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=4-hydroxyproline 2-epimerase 1 {ECO:0000303|PubMed:24980702};
DE Short=4Hyp 2-epimerase 1;
DE Short=4HypE 1 {ECO:0000303|PubMed:24980702};
DE EC=5.1.1.8 {ECO:0000269|PubMed:24980702};
GN Name=prdF {ECO:0000312|EMBL:BAG45919.1};
GN OrderedLocusNames=Bmul_4447 {ECO:0000312|EMBL:ABX18127.1},
GN BMULJ_04062 {ECO:0000312|EMBL:BAG45919.1};
OS Burkholderia multivorans (strain ATCC 17616 / 249).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=395019;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17616 / 249;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J.,
RA Richardson P.;
RT "Complete sequence of chromosome 2 of Burkholderia multivorans ATCC
RT 17616.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17616 / 249;
RA Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E.,
RA Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N.,
RA Hattori M., Tsuda M.;
RT "Complete genome sequence of Burkholderia multivorans ATCC 17616.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=24980702; DOI=10.7554/elife.03275;
RA Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B.,
RA San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A.,
RA Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.;
RT "Prediction and characterization of enzymatic activities guided by sequence
RT similarity and genome neighborhood networks.";
RL Elife 3:E03275-E03275(2014).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH
RP PYRROLE-2-CARBOXYLATE.
RG Enzyme Function Initiative (EFI);
RA Patskovsky Y., Toro R., Bhosle R., Al Obaidi N., Sojitra S., Stead M.,
RA Washington E., Glenn A.S., Chowdhury S., Evans B., Hammonds J.,
RA Hillerich B., Love J., Seidel R.D., Gerlt J.A., Almo S.C.;
RT "Crystal structure of proline racemase Bmul_4447 from Burkholderia
RT multivorans, target EFI-506586.";
RL Submitted (APR-2014) to the PDB data bank.
CC -!- FUNCTION: Catalyzes the epimerization of trans-4-hydroxy-L-proline
CC (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp). Is likely involved in a
CC degradation pathway that converts t4LHyp to alpha-ketoglutarate.
CC Displays no proline racemase activity. {ECO:0000269|PubMed:24980702}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline;
CC Xref=Rhea:RHEA:21152, ChEBI:CHEBI:57690, ChEBI:CHEBI:58375;
CC EC=5.1.1.8; Evidence={ECO:0000269|PubMed:24980702};
CC -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABX18127.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000869; ABX18127.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP009386; BAG45919.1; -; Genomic_DNA.
DR PDB; 4K7X; X-ray; 1.75 A; A=1-311.
DR PDB; 4Q60; X-ray; 2.10 A; A/B=2-311.
DR PDBsum; 4K7X; -.
DR PDBsum; 4Q60; -.
DR AlphaFoldDB; B3D6W2; -.
DR SMR; B3D6W2; -.
DR STRING; 395019.Bmul_4447; -.
DR EnsemblBacteria; BAG45919; BAG45919; BMULJ_04062.
DR KEGG; bmj:BMULJ_04062; -.
DR KEGG; bmu:Bmul_4447; -.
DR eggNOG; COG3938; Bacteria.
DR HOGENOM; CLU_036729_1_0_4; -.
DR Proteomes; UP000008815; Chromosome 2.
DR GO; GO:0047580; F:4-hydroxyproline epimerase activity; IEA:UniProtKB-EC.
DR InterPro; IPR008794; Pro_racemase_fam.
DR PANTHER; PTHR33442; PTHR33442; 1.
DR Pfam; PF05544; Pro_racemase; 1.
DR PIRSF; PIRSF029792; Pro_racemase; 1.
DR SFLD; SFLDS00028; Proline_Racemase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Reference proteome.
FT CHAIN 1..311
FT /note="4-hydroxyproline 2-epimerase 1"
FT /id="PRO_0000432247"
FT ACT_SITE 89
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT ACT_SITE 237
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT BINDING 90..91
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT BINDING 209
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT BINDING 233
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT BINDING 238..239
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT STRAND 1..12
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 15..22
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 32..42
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 44..51
FT /evidence="ECO:0007829|PDB:4K7X"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 62..66
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 73..80
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 82..85
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 90..102
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 108..114
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 120..124
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 130..133
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 137..148
FT /evidence="ECO:0007829|PDB:4K7X"
FT TURN 149..151
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 152..169
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 180..197
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 202..204
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 209..214
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 219..226
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 232..235
FT /evidence="ECO:0007829|PDB:4K7X"
FT HELIX 238..250
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 260..264
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 269..277
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 280..287
FT /evidence="ECO:0007829|PDB:4K7X"
FT STRAND 289..300
FT /evidence="ECO:0007829|PDB:4K7X"
FT TURN 304..307
FT /evidence="ECO:0007829|PDB:4K7X"
SQ SEQUENCE 311 AA; 32898 MW; B0227F077C7BA1F2 CRC64;
MMKRIQIIDS HTGGEPTRLV VSGFPSLGSG TMAERRDVLA REYDRYRTAC ILEPRGSDVL
VGALLCEPVS PDAAAGVIFF NNSGYLGMCG HGTIGVVRTL HHMGRIGPGV HRIETPVGTV
EATLHDDLSV SVRNVLAYRH AKDVALDVPG YGPVRGDIAW GGNWFFLISD HGQRVAGDNV
AALTAYASAV REGLERAGIT GANGGEIDHI ELFADDPEHD SRSFVLCPGL AYDRSPCGTG
TSAKLACLAA DGKLAPGAVW RQASVIGSVF HASYVQAEGG IVPTIRGSAH LSAEATLLIE
DDDPFRWGIV S
