INSK_ECOLI
ID INSK_ECOLI Reviewed; 283 AA.
AC P19769; Q2M7M0;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Putative transposase InsK for insertion sequence element IS150;
GN Name=insK; Synonyms=dinS {ECO:0000303|PubMed:10760155};
GN OrderedLocusNames=b3558, JW3528;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2841644; DOI=10.1093/nar/16.14.6789;
RA Schwartz E., Kroeger M., Rak B.;
RT "IS150: distribution, nucleotide sequence and phylogenetic relationships of
RT a new E. coli insertion element.";
RL Nucleic Acids Res. 16:6789-6802(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2541046; DOI=10.1093/genetics/121.3.423;
RA Hall B.G., Parker L.L., Betts P.W., Dubose R.F., Sawyer S.A., Hartl D.L.;
RT "IS103, a new insertion element in Escherichia coli: characterization and
RT distribution in natural populations.";
RL Genetics 121:423-431(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP REGULATION BY LEXA, AND INDUCTION.
RC STRAIN=K12 / RW118;
RX PubMed=10760155; DOI=10.1046/j.1365-2958.2000.01826.x;
RA Fernandez De Henestrosa A.R., Ogi T., Aoyagi S., Chafin D., Hayes J.J.,
RA Ohmori H., Woodgate R.;
RT "Identification of additional genes belonging to the LexA regulon in
RT Escherichia coli.";
RL Mol. Microbiol. 35:1560-1572(2000).
CC -!- FUNCTION: Involved in the transposition of the insertion sequence
CC IS150.
CC -!- INDUCTION: A shorter open reading frame encoding a protein called DinS,
CC encoded by a 0.4 kb mRNA encoded within the insK transcript, is
CC repressed by LexA, induced by DNA damage.
CC {ECO:0000269|PubMed:10760155}.
CC -!- SIMILARITY: Belongs to the transposase IS3/IS150/IS904 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB18535.2; Type=Miscellaneous discrepancy; Note=B-168 has been translated as Asn.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X07037; CAA30086.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18535.2; ALT_SEQ; Genomic_DNA.
DR EMBL; U00096; AAC76582.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77736.1; -; Genomic_DNA.
DR RefSeq; NP_418015.1; NC_000913.3.
DR AlphaFoldDB; P19769; -.
DR IntAct; P19769; 3.
DR STRING; 511145.b3558; -.
DR PaxDb; P19769; -.
DR PRIDE; P19769; -.
DR EnsemblBacteria; AAC76582; AAC76582; b3558.
DR EnsemblBacteria; BAE77736; BAE77736; BAE77736.
DR GeneID; 948081; -.
DR KEGG; ecj:JW3528; -.
DR KEGG; eco:b3558; -.
DR PATRIC; fig|511145.12.peg.3672; -.
DR eggNOG; COG2801; Bacteria.
DR HOGENOM; CLU_027402_4_3_6; -.
DR InParanoid; P19769; -.
DR OMA; DEVKCRI; -.
DR PhylomeDB; P19769; -.
DR BioCyc; EcoCyc:G7777-MON; -.
DR PRO; PR:P19769; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR025948; HTH-like_dom.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF13276; HTH_21; 1.
DR Pfam; PF00665; rve; 1.
DR Pfam; PF13333; rve_2; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
PE 2: Evidence at transcript level;
KW DNA recombination; DNA-binding; Reference proteome; Transposable element;
KW Transposition.
FT CHAIN 1..283
FT /note="Putative transposase InsK for insertion sequence
FT element IS150"
FT /id="PRO_0000075419"
FT DOMAIN 117..279
FT /note="Integrase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT CONFLICT 213
FT /note="R -> A (in Ref. 1; CAA30086)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="R -> E (in Ref. 2; no nucleotide entry)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 283 AA; 33477 MW; 3EA86375D52C42CB CRC64;
MKVLNELRQF YPLDELLRAA EIPRSTFYYH LKALSKPDKY ADVKKRISEI YHENRGRYGY
RRVTLSLHRE GKQINHKAVQ RLMGTLSLKA AIKVKRYRSY RGEVGQTAPN VLQRDFKATR
PNEKWVTDVT EFAVNGRKLY LSPVIDLFNN EVISYSLSER PVMNMVENML DQAFKKLNPH
EHPVLHSDQG WQYRMRRYQN ILKEHGIKQS MSRKGNCLDN AVVECFFGTL KSECFYLDEF
SNISELKDAV TEYIEYYNSR RISLKLKGLT PIEYRNQTYM PRV
