INSL3_BOVIN
ID INSL3_BOVIN Reviewed; 132 AA.
AC O77801; Q3T065;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Insulin-like 3;
DE AltName: Full=Leydig insulin-like peptide;
DE Short=Ley-I-L;
DE AltName: Full=Relaxin-like factor;
DE Contains:
DE RecName: Full=Insulin-like 3 B' chain;
DE Contains:
DE RecName: Full=Insulin-like 3 B chain;
DE Contains:
DE RecName: Full=Insulin-like 3 A chain;
DE Flags: Precursor;
GN Name=INSL3; Synonyms=RLF;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8949906; DOI=10.1095/biolreprod55.6.1452;
RA Bathgate R.A.D., Balvers M., Hunt N., Ivell R.;
RT "Relaxin-like factor gene is highly expressed in the bovine ovary of the
RT cycle and pregnancy: sequence and messenger ribonucleic acid analysis.";
RL Biol. Reprod. 55:1452-1457(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 22-66 AND 107-132, PYROGLUTAMATE FORMATION AT GLN-27,
RP MASS SPECTROMETRY, AND DISULFIDE BONDS.
RX PubMed=11772026; DOI=10.1021/bi0117302;
RA Bullesbach E.E., Schwabe C.;
RT "The primary structure and the disulfide links of the bovine relaxin-like
RT factor (RLF).";
RL Biochemistry 41:274-281(2002).
CC -!- FUNCTION: Seems to play a role in testicular function. May be a trophic
CC hormone with a role in testicular descent in fetal life. Is a ligand
CC for LGR8 receptor (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. 20% of B chains include an extra N-terminal
CC pentapeptide.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in Leydig cells of the
CC testis.
CC -!- MASS SPECTROMETRY: [Insulin-like 3 B' chain]: Mass=4885.5;
CC Method=MALDI; Note=With 2 artifactual S-carboxymethylcysteines.;
CC Evidence={ECO:0000269|PubMed:11772026};
CC -!- MASS SPECTROMETRY: [Insulin-like 3 B chain]: Mass=4501.5; Method=MALDI;
CC Note=With pyrrolidone carboxylic acid at Gln-27 and 2 artifactual S-
CC carboxymethylcysteines.; Evidence={ECO:0000269|PubMed:11772026};
CC -!- MASS SPECTROMETRY: [Insulin-like 3 A chain]: Mass=3028.0; Method=MALDI;
CC Note=With 4 artifactual S-carboxymethylcysteines.;
CC Evidence={ECO:0000269|PubMed:11772026};
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR EMBL; AF094580; AAC63380.1; -; mRNA.
DR EMBL; BC102547; AAI02548.1; -; mRNA.
DR RefSeq; NP_776790.1; NM_174365.2.
DR AlphaFoldDB; O77801; -.
DR STRING; 9913.ENSBTAP00000027848; -.
DR PaxDb; O77801; -.
DR PRIDE; O77801; -.
DR Ensembl; ENSBTAT00000027848; ENSBTAP00000027848; ENSBTAG00000025775.
DR GeneID; 281870; -.
DR KEGG; bta:281870; -.
DR CTD; 3640; -.
DR VEuPathDB; HostDB:ENSBTAG00000025775; -.
DR VGNC; VGNC:106785; INSL3.
DR eggNOG; ENOG502TFQI; Eukaryota.
DR GeneTree; ENSGT00940000163613; -.
DR HOGENOM; CLU_164865_0_0_1; -.
DR InParanoid; O77801; -.
DR OMA; NPAHHCC; -.
DR OrthoDB; 1570392at2759; -.
DR TreeFam; TF106361; -.
DR Reactome; R-BTA-444821; Relaxin receptors.
DR Proteomes; UP000009136; Chromosome 7.
DR Bgee; ENSBTAG00000025775; Expressed in theca cell and 77 other tissues.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0002020; F:protease binding; IEA:Ensembl.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; IEA:Ensembl.
DR GO; GO:0090303; P:positive regulation of wound healing; IEA:Ensembl.
DR InterPro; IPR040113; INSL3.
DR InterPro; IPR043387; INSL3/INSL4.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR PANTHER; PTHR10423; PTHR10423; 1.
DR PANTHER; PTHR10423:SF6; PTHR10423:SF6; 1.
DR Pfam; PF00049; Insulin; 1.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Hormone; Pyrrolidone carboxylic acid; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:11772026"
FT PEPTIDE 22..66
FT /note="Insulin-like 3 B' chain"
FT /id="PRO_0000016130"
FT PEPTIDE 27..66
FT /note="Insulin-like 3 B chain"
FT /id="PRO_0000016131"
FT PROPEP 67..104
FT /note="C peptide like"
FT /id="PRO_0000016132"
FT PEPTIDE 107..132
FT /note="Insulin-like 3 A chain"
FT /id="PRO_0000016133"
FT MOD_RES 27
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:11772026"
FT DISULFID 34..117
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000269|PubMed:11772026"
FT DISULFID 46..130
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000269|PubMed:11772026"
FT DISULFID 116..121
FT /evidence="ECO:0000269|PubMed:11772026"
SQ SEQUENCE 132 AA; 14378 MW; A5585500C7F2241D CRC64;
MDRRPLTWAL VLLGPALAIA LGPAAAQEAP EKLCGHHFVR ALVRLCGGPR WSSEEDGRPV
AGGDRELLRW LEGQHLLHGL MASGDPVLVL APQPLPQASR HHHHRRATAI NPARHCCLSG
CTRQDLLTLC PH
