ID   INSL3_CALJA             Reviewed;         131 AA.
AC   O97937; O97938;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Insulin-like 3;
DE   AltName: Full=Leydig insulin-like peptide;
DE            Short=Ley-I-L;
DE   AltName: Full=Relaxin-like factor;
DE   Contains:
DE     RecName: Full=Insulin-like 3 B chain;
DE   Contains:
DE     RecName: Full=Insulin-like 3 A chain;
DE   Flags: Precursor;
GN   Name=INSL3; Synonyms=RLF;
OS   Callithrix jacchus (White-tufted-ear marmoset).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Platyrrhini; Cebidae;
OC   Callitrichinae; Callithrix; Callithrix.
OX   NCBI_TaxID=9483;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RX   PubMed=9916013; DOI=10.1095/biolreprod60.2.445;
RA   Zarreh-Hoshyari-Khah M., Einspanier A., Ivell R.;
RT   "Differential splicing and expression of the relaxin-like factor gene in
RT   reproductive tissues of the marmoset monkey (Callithrix jacchus).";
RL   Biol. Reprod. 60:445-453(1999).
CC   -!- FUNCTION: Seems to play a role in testicular function. May be a trophic
CC       hormone with a role in testicular descent in fetal life. Is a ligand
CC       for LGR8 receptor (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O97937-1; Sequence=Displayed;
CC       Name=2; Synonyms=Truncated;
CC         IsoId=O97937-2; Sequence=VSP_002715, VSP_002716;
CC   -!- TISSUE SPECIFICITY: Highest expression in the Leydig cells of the
CC       testis.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; AJ011961; CAA09888.1; -; Genomic_DNA.
DR   EMBL; AJ011962; CAA09888.1; JOINED; Genomic_DNA.
DR   EMBL; AJ011961; CAA09889.1; -; Genomic_DNA.
DR   RefSeq; XP_017823698.1; XM_017968209.1. [O97937-1]
DR   AlphaFoldDB; O97937; -.
DR   STRING; 9483.ENSCJAP00000025739; -.
DR   Ensembl; ENSCJAT00000134873; ENSCJAP00000086449; ENSCJAG00000074898. [O97937-1]
DR   GeneID; 100404744; -.
DR   KEGG; cjc:100404744; -.
DR   CTD; 3640; -.
DR   eggNOG; ENOG502TFQI; Eukaryota.
DR   GeneTree; ENSGT00940000163613; -.
DR   HOGENOM; CLU_164865_0_0_1; -.
DR   InParanoid; O97937; -.
DR   OrthoDB; 1570392at2759; -.
DR   Proteomes; UP000008225; Chromosome 22.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; IEA:InterPro.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0002020; F:protease binding; IEA:Ensembl.
DR   GO; GO:0010634; P:positive regulation of epithelial cell migration; IEA:Ensembl.
DR   GO; GO:0090303; P:positive regulation of wound healing; IEA:Ensembl.
DR   InterPro; IPR040113; INSL3.
DR   InterPro; IPR043387; INSL3/INSL4.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   PANTHER; PTHR10423; PTHR10423; 1.
DR   PANTHER; PTHR10423:SF6; PTHR10423:SF6; 1.
DR   Pfam; PF00049; Insulin; 1.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cleavage on pair of basic residues; Disulfide bond;
KW   Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         25..55
FT                   /note="Insulin-like 3 B chain"
FT                   /id="PRO_0000016134"
FT   PROPEP          58..104
FT                   /note="C peptide like"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000016135"
FT   PEPTIDE         107..131
FT                   /note="Insulin-like 3 A chain"
FT                   /id="PRO_0000016136"
FT   REGION          86..105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        34..117
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..130
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        116..121
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         64..74
FT                   /note="GELLQWLERRH -> ESHSAAQDGGQ (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002715"
FT   VAR_SEQ         75..131
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002716"
SQ   SEQUENCE   131 AA;  14253 MW;  685743CAEECF8731 CRC64;
     MDPRLPAWAL VLLGPALVFA LGPAPTPEMR EKLCGHHFVR ALVRVCGGPL WSTEARRPVA
     AGDGELLQWL ERRHLLYGLV ANSEPAPGGP GLQPMPQTSH HHRHRRAAAS NPARYCCLSG
     CSQQDLLTLC P