ID   INSL3_CANLF             Reviewed;         132 AA.
AC   Q6X7V3;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Insulin-like 3;
DE   AltName: Full=Leydig insulin-like peptide;
DE            Short=Ley-I-L;
DE   AltName: Full=Relaxin-like factor;
DE   Contains:
DE     RecName: Full=Insulin-like 3 B chain;
DE   Contains:
DE     RecName: Full=Insulin-like 3 A chain;
DE   Flags: Precursor;
GN   Name=INSL3; Synonyms=RLF;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=12890727; DOI=10.1095/biolreprod.103.019166;
RA   Truong A., Bogatcheva N.V., Schelling C., Dolf G., Agoulnik A.I.;
RT   "Isolation and expression analysis of the canine insulin-like factor 3
RT   gene.";
RL   Biol. Reprod. 69:1658-1664(2003).
CC   -!- FUNCTION: Seems to play a role in testicular function. May be a trophic
CC       hormone with a role in testicular descent in fetal life. Is a ligand
CC       for LGR8 receptor (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: More strongly expressed in testis than in ovary.
CC       {ECO:0000269|PubMed:12890727}.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; AY251013; AAP79617.1; -; mRNA.
DR   RefSeq; NP_001002962.1; NM_001002962.1.
DR   AlphaFoldDB; Q6X7V3; -.
DR   STRING; 9612.ENSCAFP00000041498; -.
DR   PaxDb; Q6X7V3; -.
DR   Ensembl; ENSCAFT00030034066; ENSCAFP00030029703; ENSCAFG00030018483.
DR   GeneID; 403436; -.
DR   KEGG; cfa:403436; -.
DR   CTD; 3640; -.
DR   eggNOG; ENOG502TFQI; Eukaryota.
DR   InParanoid; Q6X7V3; -.
DR   OrthoDB; 1570392at2759; -.
DR   Proteomes; UP000002254; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   InterPro; IPR040113; INSL3.
DR   InterPro; IPR043387; INSL3/INSL4.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   PANTHER; PTHR10423; PTHR10423; 1.
DR   PANTHER; PTHR10423:SF6; PTHR10423:SF6; 1.
DR   Pfam; PF00049; Insulin; 1.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   2: Evidence at transcript level;
KW   Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         25..58
FT                   /note="Insulin-like 3 B chain"
FT                   /id="PRO_0000016137"
FT   PROPEP          61..104
FT                   /note="C peptide like"
FT                   /id="PRO_0000016138"
FT   PEPTIDE         107..132
FT                   /note="Insulin-like 3 A chain"
FT                   /id="PRO_0000016139"
FT   DISULFID        36..117
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        48..130
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        116..121
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   132 AA;  14148 MW;  5E696B1B147D2A23 CRC64;
     MSPRPLAWAL VLLGAALAVA LALGSAPAPG AREKLCGHHF VRALVRVCGG PRWSSEDGRR
     VAGGDRELLQ WLEGRHLHGQ VSDGDPMLVL VPQALPQASL HHHHRRAAAT NPAHYCCLSG
     CSRQDLLTLC PH