INSL3_MOUSE
ID INSL3_MOUSE Reviewed; 122 AA.
AC O09107; P97744;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2001, sequence version 2.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=Insulin-like 3;
DE AltName: Full=Leydig insulin-like peptide;
DE Short=Ley-I-L;
DE AltName: Full=Relaxin-like factor;
DE Contains:
DE RecName: Full=Insulin-like 3 B chain;
DE Contains:
DE RecName: Full=Insulin-like 3 A chain;
DE Flags: Precursor;
GN Name=Insl3; Synonyms=Rlf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RA Zimmermann S.H.;
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8770925; DOI=10.1210/endo.137.7.8770925;
RA Pusch W., Balvers M., Ivell R.;
RT "Molecular cloning and expression of the relaxin-like factor from the mouse
RT testis.";
RL Endocrinology 137:3009-3013(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=9428631; DOI=10.1016/s0014-5793(97)01454-3;
RA Koskimies P., Spiess A.N., Lahti P., Huhtaniemi I., Ivell R.;
RT "The mouse relaxin-like factor gene and its promoter are located within the
RT 3' region of the JAK3 genomic sequence.";
RL FEBS Lett. 419:186-190(1997).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10391220; DOI=10.1038/10364;
RA Nef S., Parada L.F.;
RT "Cryptorchidism in mice mutant for Insl3.";
RL Nat. Genet. 22:295-299(1999).
RN [5]
RP FUNCTION.
RX PubMed=11342953; DOI=10.1016/s0022-5347(05)66389-6;
RA Kubota Y., Nef S., Farmer P.J., Temelcos C., Parada L.F., Hutson J.M.;
RT "Leydig insulin-like hormone, gubernacular development and testicular
RT descent.";
RL J. Urol. 165:1673-1675(2001).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Seems to play a role in testicular function. May be a trophic
CC hormone with a role in testicular descent in fetal life. Is a ligand
CC for LGR8 receptor (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:10391220, ECO:0000269|PubMed:11342953}.
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in Leydig cells of the
CC testis.
CC -!- DISRUPTION PHENOTYPE: Male mice exhibit bilateral abdominal
CC cryptorchidism due to alteration of gubernaculum development.
CC {ECO:0000269|PubMed:10391220}.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X95603; CAA64861.1; -; Genomic_DNA.
DR EMBL; S82815; AAB39365.1; -; mRNA.
DR EMBL; AF136524; AAD24585.1; -; Genomic_DNA.
DR CCDS; CCDS22404.1; -.
DR AlphaFoldDB; O09107; -.
DR STRING; 10090.ENSMUSP00000034261; -.
DR PaxDb; O09107; -.
DR PRIDE; O09107; -.
DR ProteomicsDB; 269065; -.
DR MGI; MGI:108427; Insl3.
DR eggNOG; ENOG502TFQI; Eukaryota.
DR InParanoid; O09107; -.
DR PhylomeDB; O09107; -.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR Reactome; R-MMU-444821; Relaxin receptors.
DR PRO; PR:O09107; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; O09107; protein.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0001664; F:G protein-coupled receptor binding; ISO:MGI.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0008584; P:male gonad development; IMP:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0001556; P:oocyte maturation; ISO:MGI.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; ISO:MGI.
DR GO; GO:0090303; P:positive regulation of wound healing; ISO:MGI.
DR GO; GO:2000018; P:regulation of male gonad development; IMP:CACAO.
DR InterPro; IPR040113; INSL3.
DR InterPro; IPR043387; INSL3/INSL4.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR InterPro; IPR022352; Insulin_family.
DR PANTHER; PTHR10423; PTHR10423; 1.
DR PANTHER; PTHR10423:SF6; PTHR10423:SF6; 1.
DR Pfam; PF00049; Insulin; 1.
DR PRINTS; PR00276; INSULINFAMLY.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT PEPTIDE 16..?
FT /note="Insulin-like 3 B chain"
FT /id="PRO_0000016143"
FT PROPEP ?..94
FT /note="C peptide like"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016144"
FT PEPTIDE 97..122
FT /note="Insulin-like 3 A chain"
FT /id="PRO_0000016145"
FT DISULFID 29..107
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 41..120
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 106..111
FT /evidence="ECO:0000250"
FT CONFLICT 21
FT /note="P -> T (in Ref. 1; CAA64861)"
FT /evidence="ECO:0000305"
FT CONFLICT 54..58
FT /note="VETRD -> CGDPG (in Ref. 1; CAA64861)"
FT /evidence="ECO:0000305"
FT CONFLICT 73
FT /note="A -> S (in Ref. 1; CAA64861)"
FT /evidence="ECO:0000305"
FT CONFLICT 92..96
FT /note="QRQRR -> HARG (in Ref. 1; CAA64861)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 122 AA; 13586 MW; 10783AB4896CF103 CRC64;
MRAPLLLMLL ALGSALRSPQ PPEARAKLCG HHLVRTLVRV CGGPRWSPEA TQPVETRDRE
LLQWLEQRHL LHALVADVDP ALDPQLPRQA SQRQRRSAAT NAVHRCCLTG CTQQDLLGLC
PH
