INSL3_PIG
ID INSL3_PIG Reviewed; 131 AA.
AC P51461;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Insulin-like 3;
DE AltName: Full=Leydig insulin-like peptide;
DE Short=Ley-I-L;
DE AltName: Full=Relaxin-like factor;
DE Contains:
DE RecName: Full=Insulin-like 3 B chain;
DE Contains:
DE RecName: Full=Insulin-like 3 A chain;
DE Flags: Precursor;
GN Name=INSL3; Synonyms=RLF;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8253799; DOI=10.1016/s0021-9258(19)74364-6;
RA Adham I.M., Burkhardt E., Benahmed M., Engel W.;
RT "Cloning of a cDNA for a novel insulin-like peptide of the testicular
RT Leydig cells.";
RL J. Biol. Chem. 268:26668-26672(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8020942; DOI=10.1006/geno.1994.1121;
RA Burkhardt E., Adham I.M., Brosig B., Gastmann A., Mattei M.-G., Engel W.;
RT "Structural organization of the porcine and human genes coding for a Leydig
RT cell-specific insulin-like peptide (LEY I-L) and chromosomal localization
RT of the human gene (INSL3).";
RL Genomics 20:13-19(1994).
CC -!- FUNCTION: Seems to play a role in testicular function. May be a trophic
CC hormone with a role in testicular descent in fetal life. Is a ligand
CC for LGR8 receptor (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in prenatal and postnatal
CC Leydig cells.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X68369; CAA48449.1; -; mRNA.
DR EMBL; X73636; CAA52016.1; -; Genomic_DNA.
DR PIR; A53024; A53024.
DR RefSeq; NP_999135.1; NM_213970.1.
DR AlphaFoldDB; P51461; -.
DR STRING; 9823.ENSSSCP00000014767; -.
DR PaxDb; P51461; -.
DR Ensembl; ENSSSCT00000015173; ENSSSCP00000014767; ENSSSCG00000013887.
DR Ensembl; ENSSSCT00015109318; ENSSSCP00015046461; ENSSSCG00015080416.
DR Ensembl; ENSSSCT00025063112; ENSSSCP00025026866; ENSSSCG00025046452.
DR Ensembl; ENSSSCT00030077875; ENSSSCP00030035579; ENSSSCG00030055891.
DR Ensembl; ENSSSCT00035027250; ENSSSCP00035010421; ENSSSCG00035020931.
DR Ensembl; ENSSSCT00040065681; ENSSSCP00040027817; ENSSSCG00040048743.
DR Ensembl; ENSSSCT00045037752; ENSSSCP00045026242; ENSSSCG00045022115.
DR Ensembl; ENSSSCT00050097668; ENSSSCP00050042083; ENSSSCG00050071610.
DR Ensembl; ENSSSCT00055052190; ENSSSCP00055041698; ENSSSCG00055026398.
DR Ensembl; ENSSSCT00060036887; ENSSSCP00060015703; ENSSSCG00060027239.
DR Ensembl; ENSSSCT00065010555; ENSSSCP00065004386; ENSSSCG00065007868.
DR Ensembl; ENSSSCT00070031208; ENSSSCP00070026013; ENSSSCG00070015894.
DR GeneID; 397024; -.
DR KEGG; ssc:397024; -.
DR CTD; 3640; -.
DR VGNC; VGNC:107147; INSL3.
DR eggNOG; ENOG502TFQI; Eukaryota.
DR GeneTree; ENSGT00940000163613; -.
DR HOGENOM; CLU_164865_0_0_1; -.
DR InParanoid; P51461; -.
DR OMA; NPAHHCC; -.
DR OrthoDB; 1570392at2759; -.
DR TreeFam; TF106361; -.
DR Reactome; R-SSC-418555; G alpha (s) signalling events.
DR Reactome; R-SSC-444821; Relaxin receptors.
DR Proteomes; UP000008227; Chromosome 2.
DR Proteomes; UP000314985; Chromosome 2.
DR Bgee; ENSSSCG00000013887; Expressed in testis and 6 other tissues.
DR Genevisible; P51461; SS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0002020; F:protease binding; IEA:Ensembl.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; IEA:Ensembl.
DR GO; GO:0090303; P:positive regulation of wound healing; IEA:Ensembl.
DR InterPro; IPR040113; INSL3.
DR InterPro; IPR043387; INSL3/INSL4.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR PANTHER; PTHR10423; PTHR10423; 1.
DR PANTHER; PTHR10423:SF6; PTHR10423:SF6; 1.
DR Pfam; PF00049; Insulin; 1.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000250"
FT PEPTIDE 27..56
FT /note="Insulin-like 3 B chain"
FT /id="PRO_0000016149"
FT PROPEP 58..103
FT /note="C peptide like"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016150"
FT PEPTIDE 106..131
FT /note="Insulin-like 3 A chain"
FT /id="PRO_0000016151"
FT DISULFID 34..116
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 46..129
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 115..120
FT /evidence="ECO:0000250"
SQ SEQUENCE 131 AA; 14134 MW; 8AB718870859EF3A CRC64;
MDPHPLTWAL VLLGPALALS RAPAPAQEAP EKLCGHHFVR ALVRLCGGPR WSPEDGRAVA
GGDRELLQWL EGQHLFHGLM ASGDPMLVLA PQPPPQASGH HHHRRAAATN PARHCCLSGC
TRQDLLTLCP H
