INSL4_DROME
ID INSL4_DROME Reviewed; 134 AA.
AC Q9VT53;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Probable insulin-like peptide 4;
DE Short=dILP4;
DE AltName: Full=Insulin-related peptide 4;
DE Contains:
DE RecName: Full=Probable insulin-like peptide 4 A chain;
DE Contains:
DE RecName: Full=Probable insulin-like peptide 4 B chain;
DE Flags: Precursor;
GN Name=Ilp4; ORFNames=CG6736;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11250149; DOI=10.1016/s0960-9822(01)00068-9;
RA Brogiolo W., Stocker H., Ikeya T., Rintelen F., Fernandez R., Hafen E.;
RT "An evolutionarily conserved function of the Drosophila insulin receptor
RT and insulin-like peptides in growth control.";
RL Curr. Biol. 11:213-221(2001).
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at a high level in the embryonic
CC mesoderm, with expression continuing after gastrulation and reducing
CC from stage 12 onwards. Highly expressed in the embryonic anterior
CC midgut rudiment and larval midgut. {ECO:0000269|PubMed:11250149}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the embryo (beginning at the
CC blastoderm stage), and in the larva. {ECO:0000269|PubMed:11250149}.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF50202.1; -; Genomic_DNA.
DR EMBL; AY071235; AAL48857.1; -; mRNA.
DR RefSeq; NP_648361.1; NM_140104.3.
DR AlphaFoldDB; Q9VT53; -.
DR BioGRID; 64540; 1.
DR IntAct; Q9VT53; 2.
DR STRING; 7227.FBpp0076101; -.
DR PaxDb; Q9VT53; -.
DR DNASU; 39152; -.
DR EnsemblMetazoa; FBtr0076372; FBpp0076101; FBgn0044049.
DR GeneID; 39152; -.
DR KEGG; dme:Dmel_CG6736; -.
DR CTD; 39152; -.
DR FlyBase; FBgn0044049; Ilp4.
DR VEuPathDB; VectorBase:FBgn0044049; -.
DR eggNOG; ENOG502T8YM; Eukaryota.
DR HOGENOM; CLU_146931_0_0_1; -.
DR InParanoid; Q9VT53; -.
DR OMA; VNGFSHR; -.
DR OrthoDB; 1570392at2759; -.
DR PhylomeDB; Q9VT53; -.
DR Reactome; R-DME-110478; Insulin signaling pathway.
DR Reactome; R-DME-418555; G alpha (s) signalling events.
DR Reactome; R-DME-444821; Relaxin receptors.
DR SignaLink; Q9VT53; -.
DR BioGRID-ORCS; 39152; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39152; -.
DR PRO; PR:Q9VT53; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0044049; Expressed in stage 5 embryo (Drosophila) and 29 other tissues.
DR ExpressionAtlas; Q9VT53; baseline and differential.
DR Genevisible; Q9VT53; DM.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; ISS:FlyBase.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR GO; GO:0005158; F:insulin receptor binding; ISS:UniProtKB.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0008286; P:insulin receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0030536; P:larval feeding behavior; IMP:FlyBase.
DR InterPro; IPR043387; INSL3/INSL4.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR InterPro; IPR022352; Insulin_family.
DR PANTHER; PTHR10423; PTHR10423; 1.
DR Pfam; PF00049; Insulin; 1.
DR PRINTS; PR00276; INSULINFAMLY.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..134
FT /note="Probable insulin-like peptide 4"
FT /id="PRO_0000016197"
FT PEPTIDE 27..48
FT /note="Probable insulin-like peptide 4 B chain"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016198"
FT PROPEP 54..108
FT /note="Connecting peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016199"
FT PEPTIDE 115..134
FT /note="Probable insulin-like peptide 4 A chain"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016200"
FT REGION 72..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..95
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 31..120
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 43..133
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 119..124
FT /evidence="ECO:0000250"
SQ SEQUENCE 134 AA; 15140 MW; AE8D916950C16075 CRC64;
MSLIRLGLAL LLLLATVSQL LQPVQGRRKM CGEALIQALD VICVNGFTRR VRRSSASKDA
RVRDLIRKLQ QPDEDIEQET ETGRLKQKHT DADTEKGVPP AVGSGRKLRR HRRRIAHECC
KEGCTYDDIL DYCA
