INSL5_MOUSE
ID INSL5_MOUSE Reviewed; 135 AA.
AC Q9WUG6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Insulin-like peptide INSL5;
DE Short=Insulin-like peptide 5;
DE AltName: Full=Relaxin/insulin-like factor 2;
DE AltName: Full=Relaxin/insulin-like protein;
DE Contains:
DE RecName: Full=Insulin-like peptide INSL5 B chain;
DE Contains:
DE RecName: Full=Insulin-like peptide INSL5 A chain;
DE Flags: Precursor;
GN Name=Insl5; Synonyms=Rif, Rif2, Zins3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Colon;
RX PubMed=10458910; DOI=10.1006/geno.1999.5899;
RA Conklin D., Lofton-Day C.E., Haldeman B.A., Ching A., Whitmore T.E.,
RA Lok S., Jaspers S.;
RT "Identification of INSL5, a new member of the insulin superfamily.";
RL Genomics 60:50-56(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=FVB/NJ; TISSUE=Ovary;
RX PubMed=10598589; DOI=10.1210/mend.13.12.0388;
RA Hsu S.Y.;
RT "Cloning of two novel mammalian paralogs of relaxin/insulin family proteins
RT and their expression in testis and kidney.";
RL Mol. Endocrinol. 13:2163-2174(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May have a role in gut contractility or in thymic development
CC and regulation. Activates RXFP4 with high potency and appears to be the
CC endogenous ligand for this receptor (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highest expression in colon with lower levels in
CC thymus. Minimal levels in testis. {ECO:0000269|PubMed:10458910}.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF09093.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF133817; AAD29687.1; -; mRNA.
DR EMBL; AF076971; AAD48089.1; -; mRNA.
DR EMBL; AF054842; AAF09093.1; ALT_INIT; mRNA.
DR EMBL; AF054843; AAF09094.1; -; mRNA.
DR EMBL; BC010968; AAH10968.1; -; mRNA.
DR CCDS; CCDS18406.1; -.
DR RefSeq; NP_001277577.1; NM_001290648.1.
DR RefSeq; NP_035961.1; NM_011831.2.
DR AlphaFoldDB; Q9WUG6; -.
DR SMR; Q9WUG6; -.
DR STRING; 10090.ENSMUSP00000081416; -.
DR PaxDb; Q9WUG6; -.
DR PRIDE; Q9WUG6; -.
DR ProteomicsDB; 269320; -.
DR Antibodypedia; 33391; 69 antibodies from 20 providers.
DR DNASU; 23919; -.
DR Ensembl; ENSMUST00000084382; ENSMUSP00000081416; ENSMUSG00000066090.
DR GeneID; 23919; -.
DR KEGG; mmu:23919; -.
DR UCSC; uc008txa.1; mouse.
DR CTD; 10022; -.
DR MGI; MGI:1346085; Insl5.
DR VEuPathDB; HostDB:ENSMUSG00000066090; -.
DR eggNOG; ENOG502S9UT; Eukaryota.
DR GeneTree; ENSGT00940000154396; -.
DR InParanoid; Q9WUG6; -.
DR OMA; YVRTVVY; -.
DR OrthoDB; 1578838at2759; -.
DR TreeFam; TF333404; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-444821; Relaxin receptors.
DR BioGRID-ORCS; 23919; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Insl5; mouse.
DR PRO; PR:Q9WUG6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9WUG6; protein.
DR Bgee; ENSMUSG00000066090; Expressed in left colon and 45 other tissues.
DR ExpressionAtlas; Q9WUG6; baseline and differential.
DR Genevisible; Q9WUG6; MM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:2000253; P:positive regulation of feeding behavior; IDA:MGI.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR Pfam; PF00049; Insulin; 1.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..46
FT /note="Insulin-like peptide INSL5 B chain"
FT /id="PRO_0000016166"
FT PROPEP 49..114
FT /note="Connecting peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016167"
FT PEPTIDE 115..135
FT /note="Insulin-like peptide INSL5 A chain"
FT /id="PRO_0000016168"
FT DISULFID 29..122
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 41..135
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 121..126
FT /evidence="ECO:0000250"
SQ SEQUENCE 135 AA; 15524 MW; 05FF9A0F613DBF92 CRC64;
MKGPTLALFL LLVLLAVVEV RSRQTVKLCG LDYVRTVIYI CASSRWRRHL EGHFHSQQAE
TRNYLQLLDR HEPSKKTLEH SLPKTDLSGQ ELVRDPQAPK EGLWELKKHS VVSRRDLQAL
CCREGCSMKE LSTLC
