INSL6_HUMAN
ID INSL6_HUMAN Reviewed; 213 AA.
AC Q9Y581; A0AVS0; Q9NS16;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2002, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Insulin-like peptide INSL6;
DE Short=Insulin-like peptide 6;
DE AltName: Full=Relaxin/insulin-like factor 1;
DE Contains:
DE RecName: Full=Insulin-like peptide INSL6 B chain;
DE Contains:
DE RecName: Full=Insulin-like peptide INSL6 A chain;
DE Flags: Precursor;
GN Name=INSL6; Synonyms=RIF1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=10598589; DOI=10.1210/mend.13.12.0388;
RA Hsu S.Y.;
RT "Cloning of two novel mammalian paralogs of relaxin/insulin family proteins
RT and their expression in testis and kidney.";
RL Mol. Endocrinol. 13:2163-2174(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=10819760; DOI=10.1095/biolreprod62.6.1593;
RA Lok S., Johnston D.S., Conklin D., Lofton-Day C.E., Adams R.L.,
RA Jelmberg A.C., Whitmore T.E., Schrader S., Griswold M.D., Jaspers S.R.;
RT "Identification of INSL6, a new member of the insulin family that is
RT expressed in the testis of the human and Rat.";
RL Biol. Reprod. 62:1593-1599(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May have a role in sperm development and fertilization.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Testis specific.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR EMBL; AF135824; AAF29604.1; -; mRNA.
DR EMBL; AF156094; AAD39003.1; -; mRNA.
DR EMBL; AL161450; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC126473; AAI26474.1; -; mRNA.
DR EMBL; BC126475; AAI26476.1; -; mRNA.
DR CCDS; CCDS6458.1; -.
DR RefSeq; NP_009110.2; NM_007179.2.
DR AlphaFoldDB; Q9Y581; -.
DR SMR; Q9Y581; -.
DR BioGRID; 116343; 27.
DR IntAct; Q9Y581; 18.
DR STRING; 9606.ENSP00000371054; -.
DR PhosphoSitePlus; Q9Y581; -.
DR BioMuta; INSL6; -.
DR DMDM; 20178309; -.
DR MassIVE; Q9Y581; -.
DR PaxDb; Q9Y581; -.
DR PeptideAtlas; Q9Y581; -.
DR PRIDE; Q9Y581; -.
DR ProteomicsDB; 86312; -.
DR Antibodypedia; 9538; 45 antibodies from 15 providers.
DR DNASU; 11172; -.
DR Ensembl; ENST00000381641.4; ENSP00000371054.3; ENSG00000120210.8.
DR GeneID; 11172; -.
DR KEGG; hsa:11172; -.
DR MANE-Select; ENST00000381641.4; ENSP00000371054.3; NM_007179.3; NP_009110.2.
DR UCSC; uc003zix.4; human.
DR CTD; 11172; -.
DR DisGeNET; 11172; -.
DR GeneCards; INSL6; -.
DR HGNC; HGNC:6089; INSL6.
DR HPA; ENSG00000120210; Group enriched (retina, testis).
DR MalaCards; INSL6; -.
DR MIM; 606414; gene.
DR neXtProt; NX_Q9Y581; -.
DR OpenTargets; ENSG00000120210; -.
DR PharmGKB; PA29896; -.
DR VEuPathDB; HostDB:ENSG00000120210; -.
DR eggNOG; ENOG502RWPT; Eukaryota.
DR GeneTree; ENSGT00940000154434; -.
DR HOGENOM; CLU_1299356_0_0_1; -.
DR InParanoid; Q9Y581; -.
DR OMA; SIACFPY; -.
DR PhylomeDB; Q9Y581; -.
DR TreeFam; TF343788; -.
DR PathwayCommons; Q9Y581; -.
DR SignaLink; Q9Y581; -.
DR BioGRID-ORCS; 11172; 12 hits in 1057 CRISPR screens.
DR ChiTaRS; INSL6; human.
DR GenomeRNAi; 11172; -.
DR Pharos; Q9Y581; Tdark.
DR PRO; PR:Q9Y581; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q9Y581; protein.
DR Bgee; ENSG00000120210; Expressed in left testis and 93 other tissues.
DR ExpressionAtlas; Q9Y581; baseline and differential.
DR Genevisible; Q9Y581; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005179; F:hormone activity; NAS:UniProtKB.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR017100; Insulin-like_pep_6.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR PANTHER; PTHR12004:SF1; PTHR12004:SF1; 1.
DR Pfam; PF00049; Insulin; 1.
DR PIRSF; PIRSF037062; Insulin-like_peptide_6; 1.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..213
FT /note="Insulin-like peptide INSL6"
FT /id="PRO_0000016172"
FT PEPTIDE 21..53
FT /note="Insulin-like peptide INSL6 B chain"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016173"
FT PROPEP 55..168
FT /note="Connecting peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016174"
FT PEPTIDE 173..198
FT /note="Insulin-like peptide INSL6 A chain"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016175"
FT PROPEP 201..213
FT /evidence="ECO:0000255"
FT /id="PRO_0000016176"
FT DISULFID 33..179
FT /evidence="ECO:0000250"
FT DISULFID 45..192
FT /evidence="ECO:0000250"
FT DISULFID 178..183
FT /evidence="ECO:0000250"
FT VARIANT 80
FT /note="F -> L (in dbSNP:rs2149554)"
FT /id="VAR_024329"
FT CONFLICT 146
FT /note="K -> F (in Ref. 2; AAD39003)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 213 AA; 24860 MW; 12A097659CFAC471 CRC64;
MPRLLRLSLL WLGLLLVRFS RELSDISSAR KLCGRYLVKE IEKLCGHANW SQFRFEEETP
FSRLIAQASE KVEAYSPYQF ESPQTASPAR GRGTNPVSTS WEEAVNSWEM QSLPEYKDKK
GYSPLGKTRE FSSSHNINVY IHENAKFQKK RRNKIKTLSN LFWGHHPQRK RRGYSEKCCL
TGCTKEELSI ACLPYIDFKR LKEKRSSLVT KIY
