INSQ_ECOLI
ID INSQ_ECOLI Reviewed; 402 AA.
AC P76102; P77144;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 3.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative RNA-guided DNA endonuclease InsQ {ECO:0000250|UniProtKB:Q7DF80};
DE EC=3.1.21.- {ECO:0000250|UniProtKB:Q7DF80};
GN Name=insQ; Synonyms=tnpB, ydcM; OrderedLocusNames=b1432, JW5228;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: An RNA-guided dsDNA endonuclease. When guided by an RNA
CC derived from the right-end element of its insertion sequence element
CC (IS), cleaves DNA downstream of the transposon-associated motif (TAM).
CC Cleaves supercoiled and linear DNA in a staggered manner 15-21 bases
CC from the TAM yielding 5'-overhangs. Binds reRNA, an approximately 150
CC nucleotide base sRNA derived from the 3' end of its own gene, the right
CC end (RE) of the insertion sequence (IS) plus sequence downstream of the
CC IS. {ECO:0000250|UniProtKB:Q7DF80}.
CC -!- FUNCTION: Not required for transposition of the insertion element. The
CC corresponding transposase in strains MG1655 and W3110 is a truncated
CC pseudogene (yncK). {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-21 is the initiator; the
CC sequence between Met-1 and Met-21 is conserved compared to orthologs
CC but does not have a good ribosome-binding site (RBS) whereas Met-21 has
CC a better RBS. Perhaps both are used, with translation initiating more
CC often at the second Met. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC74514.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00096; AAC74514.2; ALT_INIT; Genomic_DNA.
DR EMBL; AP009048; BAA15060.2; -; Genomic_DNA.
DR PIR; C64895; C64895.
DR RefSeq; NP_415949.2; NC_000913.3.
DR AlphaFoldDB; P76102; -.
DR BioGRID; 4260186; 183.
DR BioGRID; 849339; 1.
DR DIP; DIP-48268N; -.
DR IntAct; P76102; 1.
DR STRING; 511145.b1432; -.
DR PaxDb; P76102; -.
DR PRIDE; P76102; -.
DR EnsemblBacteria; AAC74514; AAC74514; b1432.
DR EnsemblBacteria; BAA15060; BAA15060; BAA15060.
DR GeneID; 944942; -.
DR KEGG; ecj:JW5228; -.
DR KEGG; eco:b1432; -.
DR PATRIC; fig|1411691.4.peg.837; -.
DR EchoBASE; EB3519; -.
DR eggNOG; COG0675; Bacteria.
DR HOGENOM; CLU_032903_0_4_6; -.
DR InParanoid; P76102; -.
DR PhylomeDB; P76102; -.
DR BioCyc; EcoCyc:G6743-MON; -.
DR PRO; PR:P76102; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR001959; Transposase_2.
DR InterPro; IPR010095; Transposase_IS605_OrfB_C.
DR InterPro; IPR021027; Transposase_put_HTH.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
DR TIGRFAMs; TIGR01766; tspaseT_teng_C; 1.
PE 3: Inferred from homology;
KW DNA recombination; DNA-binding; Endonuclease; Hydrolase; Metal-binding;
KW Nuclease; Reference proteome; Transposable element; Transposition; Zinc.
FT CHAIN 1..402
FT /note="Putative RNA-guided DNA endonuclease InsQ"
FT /id="PRO_0000201319"
FT ACT_SITE 183
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT ACT_SITE 267
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT ACT_SITE 363
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT BINDING 334
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT BINDING 337
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT BINDING 353
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT BINDING 356
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q7DF80"
SQ SEQUENCE 402 AA; 45741 MW; CDE74032DF5E61B5 CRC64;
MKRLQAFKFQ LRPGGQQECE MRRFAGACRF VFNRALARQN ENHEAGNKYI PYGKMASWLV
EWKNATETQW LKDSPSQPLQ QSLKDLERAY KNFFRKRAAF PRFKKRGQND AFRYPQGVKL
DQENSRIFLP KLGWMRYRNS RQVTGVVKNV TVSQSCGKWY ISIQTESEVS TPVHPSASMV
GLDAGVAKLA TLSDGTVFEP VNSFQKNQKK LARLQRQLSR KVKFSNNWQK QKRKIQRLHS
CIANIRRDYL HKVTTAVSKN HAMIVIEDLK VSNMSKSAAG TVSQPGRNVR AKSGLNRSIL
DQGWYEMRRQ LAYKQLWRGG QVLAVPPAYT SQRCAYCGHT AKENRLSQSK FRCQVCGYTA
NADVNGARNI LAAGHAVLAC GEMVQSGRPL KQEPTEMIQA TA
